Exam #3 (Transcription- Genetic tools)

Question 1

A mutation in the start codon would likely affect the activity of which of the following proteins?

A. Ribosome
B. Sigma 70 (Sigma A)
C. RNA polymerase
D. DNA polymerase III

Answer 1

A.

Question 2

Rho-independent termination depends on the stop codon to terminate transcription.
T/F

Answer 2

False

Question 3

Where does DNA replication begin on the bacterial chromosome?
A. oriV
B. oriT
C. terC
D. oriC

Answer 3

D.

Question 4

What site do riboswitches and sRNA interact with to stop translation?
operator site
A. operator site
B. ATG (the start codon)
C. Shine-Dalgarno sequence
D. Promoter region

Answer 4

C.

Question 5

What would occur if the dif site on a bacterial chromosome was deleted?
A. Prevent decatenation by topoisomerase.
B. Prevent replication from initiating.
C. The chromosome cannot be conjugated.
D. The chromosome cannot be repaired by the MMR system

Answer 5

A.

Question 6

Amino acids are activated for translation when bound to what?
A. rRNA
B. tRNA
C. ATP
D. mRNA

Answer 6

B.

Question 7

In general, alternative sigma factors would be a form of what type of regulation?
A. Translational-level regulation
B. Catabolite repression
C. Transcriptional-level regulation
D. Quorum sensing

Answer 7

C.

Question 8

What is the main machinery for each type of genetic transfer?
1. Transformation
2. Conjugation
3. Transduction

Answer 8

1. Com
2. Tra
3. Bacteriophage

Question 9

If DNA polymerase III encounters a single-stranded nick, then replication will stall. What repair system will likely be used if an extra copy of the chromosome is available?
A. DNA polymerase IV
B. RecBCD pathway
C. RecFOR pathway
D. Proofreading

Answer 9

C.

Question 10

The machinery for conjugation requires machinery typically carried on the F factor, which requires what cis-acting factor for transfer?
A. oriV
B. oriT
C. oriC
D. sex pilus

Answer 10

B.

Question 11

If you wanted to know the genotype of a specific phenotype for a bacterium, then you would use 1. ________
. If you wanted to know the phenotype for a specific genotype, then you would use 2. _________

Answer 11

1. Forward genetics
2. Reverse Genetics

Question 12

For a transcriptional fusion with 2 genes, you would expect A._____ Shine-Dalgarno (SD) sequence(s), but if it was a translational fusion you would expect B._____ Shine-Dalgarno (SD) sequence(s).

Answer 12

A. 2
B. 1

Question 13

One difference between the SYBR Green and TacMan method of qPCR is:
A. TacMan can be be multiplexed
B. SYBR Green can uses taq polymerase
C. TacMan can measure initial DNA sample concentration
D. SYBR Green uses fluorescence

Answer 13

A.

Question 14

When would each repair system be activated?

1. DNA polymerase III Proofreading
2. UvrABC
3. DNA Polymerase IV
4. MutSLH + DNA polymerase I

Answer 14

1. During Replication
2. After SOS response is Activated
3. After SOS response is Activated
4. Immediatly after replication and befoe methylation

Question 15

For the process of molecular cloning with a plasmid we discussed in class, the process of 1. ________
can be considered a mechanism to copy DNA, while 2. _____
can be used to cut plasmids open to receive DNA inserts. In the end 3. _______ is used to anneal two DNA fragments together.

Answer 15

1. PCR
2. Restriction Enzymes
3. DNA ligase

Question 16

How many of the following would you expect in 1 operon with 5 genes?
1. # of Start Codons
2. # of Stop Codons
3. # of Promoters
4. # of Transcriptional Terminators
5. # of Shine-Dalgarno sequences

Answer 16

1. 5
2. 5
3. 1
4. 1
5. 5