Lecture 12 Flashcards
Diploid life cycle general
Starting with haploid stage
Gametes (1n)(sperm and egg)
Fertilization
Zygote 2n (many mitosis after this)
Mature 2n
Meiosis
Repeat
Alternation of generation life cycle simple
Starting with meiosis
Meiosis
Spores 1n
Many mitosis
Mature 1n
Mitosis
Gametes 1n (sperm/egg)
Fertilization
Zygote 2n
Many mitosis
Mature2n
Repeat
Spirit meiosis
Direct product of meiosis is 4 haploid spores not gametes
Germinate
Spores germinate and grow into a haploid plant
Sperm and eggs in alternation of generations is formed by
Mitosis
When mature haploid plant produces sperm/ egg which fuse to make a zygote
Fungi and algae life cycle simple
Starting with meiosis
Meiosis
Spore
Many mitosis
Mature 1n
Mitosis
Gametes 1n
Fertilization
Zygote 2n
No mitosis
Repeat
Zygotic meiosis
Because zygote immediately does meiosis. It doesn’t grow at all (which would be achieved by many mitosis)
Fungi and algae meiosis produce what
4 spores
Metabolism carries out largely by
Enzymes
Anabolism
Building something up
Ex photosynthesis. Lipid synthesis
Catabolism
Breaking down something
Ex. Cellular respiration. Glycolysis
2 major types of energy
Kinetic energy
Potential energy
Thermodynamics
Describes energy and its transformation
Looks at things as a system and its surroundings
What kind of systems are biological system
Open systems
Open systems definition
Systems that exchange matter and energy with surroundings
Ex. Nucleus
Cell
Goat
Ecosystem
First law of thermodynamics
Energy can’t be created or destroyed
Only transformed and transfered
Entropy
Energy and matter move from ordered to disordered. (They spread out over time)(eg. Diffusion)
Amount of disorder call entropy (s)
Entropy symbol
Definition
S
Amount of disorder call
Rank entropy in solid vs liquid vs gas
Solid least entropy
Liquid middle
Gas highest entropy
Second law of thermodynamics
Entropy of the universe is continuously increasing
Gibbs free energy (definition and symbol)
G
Amount is energy available in a system to do work
What happens to free energy when a boulder rolls down a hill
Loses free energy
Negative delta G
System loses free energy
Negative delta G
Exergonic
System gains free energy
+ delta G
Endergonic
Exergonic
Loss of free energy
Endergonic
Gaining free energy
Second degree active transport
Energy released from 1 ion flowing down concentration gradient
Use that energy to drive the other ion against its concentration gradient.
Spontaneous reaction
Chemical rxn that will proceed on its own without outside influence
Exergonic reaction
Energy releasing chemical rxn yielding products that contain less potential energy than their reactants
Endergonic reaction
Energy-requiring chemical rxn yielding products rich in potential energy than
Energy coupling reaction
Use of wnergy released from Exergonic reactions to drive essential Endergonic reaction
Energy of activiation
EA
amount of energy reactants must absorb before a chemical reaction will start
This represent the energy barrier that prevents molecules from breaking down spontaneously
Role of enzymes
Lowering activation energy of specific biochemical reactions
Enzyme
Protein that serves as a biological catalyst
Catalyst
Substance which can lower the energy of activation
Enzyme does what
Lower activation energy of the biochemical rxn
Allows equilibrium to be approached at a fast rate
Will an enzyme be turned into a product after rxn
No
Lock/key principle
Specificity between enzyme and substrate
Substrate
Where an enzyme acts
Active site
Region on the surface of an enzyme where the substrate binds and where catalysis occurs
Enzymes are usually built how
Single polypeptide or protein complex
Many enzymes also require presence of what
Other non protein molecules
Cofactor
Coenzyme
Prosthetic group
Cofactor
Inorganic ions such as iron. Copper. Zinc that bind to certain enzymes
Coenzyme
Small carbon containing molecules such as
Coenzyme A (CoA)
NAD
FAD
ATP
Which aren’t permanently bound to the enzyme and must collide with the enzyme and bind to its active site before the catalytic reaction occurs
Prosthetic group
These distinctive molecular group are permanently bound to their enzymes such as heme or flavin
Enzyme activity may be regulated how
Inhibitors
pH
Temperature
Etc
Bioenergy carriers
Example
ATP
ATP
Adenosine 5’- triphosphate
-Energy currency/shuttle of living cell
-living cell stores energy in chemical bonds of lipid starc glycogen (like a bank account)
-energy the cell requires for immediate use is temporarily stored in ATP (cash in pocket)
Cell continuously spends ATP which must be replaced immediately
Structure of ATP
Nucleotide consisting of 3 main parts
-adenine
- ribose
- three phosphate groups
Adenine
Nitrogen containing organic base
Ribose
5 carbon sugar
Phosphate groups in ATP
Identifiable as phosphorus atoms surrounded by oxygen atoms
Function of ATP
Hydrolysis of ATP- Exergonic reaction (delta G= -7.6kcal/mole)
Rxn of AGP hydrolysis also favors formation of products in living cells
Donate energy through the transfer of a phosphate group
Nearly all cellular worm depends of ATP energizing other molecules by phosphorylation (transfer of a phosphate group , usually from ATP to a molecule)
Delta G of hydrolysis of ATP
-7.6 kcal/mol
Hydrolysis of ATP what kind of rxn
Exergonic
When terminal phosphate is removed from ATP
what remains?
Adenosine diphosphate
ADP
If phosphate group from hydrolysis of ATP isn’t transferred to another molecule what happens
Released as inorganic phosphate
Exergonic reaction
Formula for hydrolysis of ATP
ATP + H2O -> ADP + Pi+ delta G
Sucrose synthesis formula and rxn type
Glucose+ Fructose+ ATP Sucrose + ADP +Pi + delta G
Generation of ATP
Substrate lvl phosphorylation
Chemiosmosis
Substrate level phosphorylation
ATP formed when a phosphate group is transferred to ADP from a phosphorylated intermediate
Chemiosmosis
Process by which phosphorylation of ADP to form ATP isn’t coupled to the transfer of electrons down an electron transport chain
-oxidative phosphorylation
- photophosporylation
Oxidative phosphorylation
ATP synthesis driven by electron transfer to oxygen
Photophosporylation
ATP synthesis driven by light