Lecture 11-12

Question 1

Enzyme

Answer 1

Protein or mina that act as a catalyst for a chemical reaction.

Question 2

Reaction rate equation

Answer 2

v = - 􏰝[S] / 􏰝􏰎t

Question 3

What factors limit rate of reaction?

Answer 3

1. ) availability of substrate

2. ) production of product

Question 4

Michealis Menton equation

Answer 4

v0 = [S] vmax

[S] + Km

Question 5

Km

Answer 5

1/2 vmaxThe Km is often near the physiological concentration of the substrate in the target cell. Enzymes with lower km values work best.

Question 6

What can Km values be used to?

Answer 6

can be used to investigate the affinity of an enzyme for a substrate and compare reaction rates in more complicated (living) systems

Question 7

Competitive inhibition

Answer 7

a compound that binds the active site of the enzyme and excludes the reactant. Vmax constant, km increase

Question 8

Uncompetitive inhibition

Answer 8

a compound binds to the ES complex and slows the release to E+R. vmax and Km decrease.

Question 9

noncompetitive inhibition

Answer 9

a compound binds the enzyme and decrease the rate the enzyme can bind to or react with the substrate. vmax reduced and km unchanged

Question 10

Enzyme activity equation-

Answer 10

(delta(c) /min) (rxn volume) / enzyme volume

Question 11

Specific activity equation

Answer 11

Activity/protein