BMSC 240 lecture 1-5

Question 1

Sensitivity

Answer 1

minimum amount of X needed to record a positive result

Question 2

Specificity

Answer 2

a positive result only comes from a truly positive sample

Question 3

random error

Answer 3

new error introduced with each

measurement

Question 4

systematic error

Answer 4

error that is consistently present

Question 5

accuracy

Answer 5

how close a recorded value is to the true value. one measurement can be accurate

Question 6

precision

Answer 6

how reliably you can measure a value. one measurement cannot be precise.

Question 7

acid

Answer 7

Proton donor–> increase concentration of H+

Question 8

base

Answer 8

Proton acceptor–> reduces concentration of H+

Question 9

pH equation

Answer 9

pH=-log[h+]

Question 10

PKA

Answer 10

At a specific pH there will be equal amounts of the acid and conjugate base in the solution

Question 11

Henderson Hasselbaf equation

Answer 11

pH = pKa + log [base]/[acid] pH = pKa + log [A-]/[HA]

Question 12

Triprotic amino acids

Answer 12

AsparticacidpKa=~4 – GlutamicacidpKa=~4 – HistidinepKa=~6
•CysteinepKa=~8
• Tyrosine pKa = ~10
• Lysine pKa = ~10
• Arginine pKa = ~12.5

Question 13

The effectiveness of killing is effected by what 3 things?

Answer 13

Temperature, time, conductance

Question 14

Quality of an image is dependant on

Answer 14

thickness of section, lighting, staining, quality/cleaniness of lens.

Question 15

Solution sterilization

Answer 15

Autoclave and filtration.

Question 16

resolution

Answer 16

the smallest resolvable distance between 2 objects

Question 17

resolution equation

Answer 17

R=0.61l/NA

Question 18

Spectrophotometry

Answer 18

Spectroscopy = the study of absorption and emission of radiation
– Photometry = the measurement of intensity of radiation

Question 19

higher energy

Answer 19

=shorter wavelength (blue)

Question 20

lower energy

Answer 20

=longer wavelength (red)