Lec5 Molecular

Question 1

Subunits of rnapol prokaryotic

Answer 1

2Alpa beta beta prime sigma and omega (W)

Question 2

C and N terminal of the rnapol pro

Answer 2

C== upstream elements
N== subunits of enzymes

Question 3

Beta subunit of rnapol pro

Answer 3

Catalytic/ ribonucleoside triphosphates
2 mg ion. One I’m active side
One in nucleotide

Question 4

Beta prime ?

Answer 4

Bind dna non specific manner

Question 5

W subunits

Answer 5

Stability chaperon like

Question 6

Domain of the Sigma and function

Answer 6

1.2.3.4. domain
In the core
One part expose outside bund dna promoter
One inside towards the clamp fbetweeb the b and b prime subunitb

Question 7

Wall

Answer 7

Bottom of the entrry grrove the rnapol pro - bend 90 degree the dna

Question 8

Bind subunits to promoter on the pro

Answer 8

Signa 4 .. 2
-35. -10

Question 9

Dna position between the —- and —- subunits

Answer 9

B and b prime

Question 10

RNA more than 10 nt ==

Answer 10

RNA polymerase transcribe

Question 11

Most frequent sigma

Answer 11

Sigma 70

Question 12

Pribnow box

Answer 12

Promoter, _10,

Question 13

Sigma 70 function

Answer 13

Recognising the -35 and -10 / pribnow box

Question 14

Pribnow box and -35 rich in

Answer 14

A and t

Question 15

rRna promoter

Answer 15

Element up …-35….-10