after 1 week

Question 1

why we can denature dna in vitrono covalent bond

Question 2

monitoring the denaturation

Answer 2

uv spectroscopy

Question 3

hyperchromic effect

Answer 3

1 satrand abdorb more uv than double

Question 4

melting t

Answer 4

half of the dna is denatureed

Question 5

dna unwinding is balanced by….

Answer 5

negative supercoil

Question 6

codons specificy the same amino acids

Answer 6

synonymous

Question 7

number of introns and size on intrones

Answer 7

related to size genomme but the size of introns just the length of introns

Question 8

elements of replication

Answer 8

replicon, replicator(origin), inititator pr (interacti with replicator)

Question 9

INTERFERENCE AND CIEFFICIENT COOINCIDENCE

Answer 9

INTERFERENCE= 1- COFFICIENT COINCIDENECE
COFFICIENT = OBSERVED DOUBLE CROSS OVER/ EXPECTED ( FREQ1 . 2 . WHOLE )

Question 10

Lineralize the branching introns after splicing

Answer 10

Debranching enzyme

Question 11

Assembly of the splicesome

Question 12

HAMMERHEAD RNA

Answer 12

RIBOZYME - HYDROLIZE THE PHOSPHODIESTER , 2-3 PRIME CYCLIC PHOPAHATE AT 3 PRRIME END AND HYDROXYL AT 5 PRIME END

Question 13

TEGULSTION OF ALTERNATIVE SPLICING

Answer 13

CIS ( SOECIFIC ELEMENTS )
TRASN (INTERACTI ITH THE CIS ELEMENTS )

Question 14

RRM

Answer 14

RNA RECOGNATION DOMAIN

Question 15

WITH WHAT DOES THE RS/HNRNP FAMILY BIND RNA

Answer 15

RRM
RRMS

Question 16

rRNA pro

Answer 16

16 , 23 , 5

Question 17

rRNA euk

Answer 17

18, 5.8 , 28

Question 18

most rna in the cell

Answer 18

rRNA

Question 19

FIRST RNA PURIFIED

Answer 19

rRNA

Question 20

clover structure

Answer 20

trna - secondary strnded

Question 21

whicg arm bind aa in trna

Answer 21

acceptor 3 prime

Question 22

TPSIC ARM

Answer 22

contain tymine and pseudouracil

Question 23

endoribonucleases in pro

Answer 23

rnase 3 for proceessing of both trna and rrna- rnase p for trna and rnase e for rrna

Question 24

nucleotidyl transferase enzyme

Answer 24

add the cca to 3 prime of the trna in euk

Question 25

introns only mrna

Answer 25

nooo trna as well !

Question 26

all splicing recuirs transesterification

Answer 26

no splicing in trna !

Question 27

start codon

Answer 27

AUG MET

Question 28

MCM2-7

Answer 28

HETEROHEXAMER RING IS FORMED
ALSI ELONGATION

Question 29

MARK TRANSITION FROM INITITATION OF THE REPLICATION TO ELONGATION IN REPLICATION OF BACTERIA

Answer 29

DISASSOCIATION DNAC FROM DNAB

Question 30

WHAT BINDS TO ARS AND IDENTIFY THE REPLICON OF ORGIN ?

Answer 30

ORC

Question 31

NUMBER OF REPLICON BETWEEN EU AND PRO

Answer 31

EU MANY AT SAME TIME ONLY FEW WILL BE CHOSSEN PROKARYOTES ONLY 1

Question 32

CTCF+COHESION EXAMPLE

Answer 32

IBIND TO INSULATOR EXMPALE OF BOUNDRY MARK BETWEEN THE EUCHROMATIN AND HETEROCHROMATIN

Question 33

ACTION OF EUK REPRESSORS

Answer 33

1- INHIBIT BINDING ACTIVATORS
2- REPRESSION ACTIVE DOMAIN HINHIBIT THE TRANSCRIPTION FACTORS
3-MODIFY THE CHROMATIN

Question 34

TERMINATION IN RNAPOL 3

Answer 34

SIMILARITY TO PRO: POLY U REGIONS

NO SECONDARY STRUCTURE !!

GC RIH

Question 34

GAL4

Answer 34

SITE OF LAC Z

Question 34

MECHANISM OF THE ACTIVATIOR

Answer 34

1- PIC FORMATION HIG DUE THE BASAL FACTOR RECUIRMENT
2-CAHNGE CHROMATIN FORMATION MAKES THE PROMOTER MORE ACCESS
3- MODIFICATION OF TRANSCRIPTION FACTOR

Question 34

GAL 1

Answer 34

ACCTIVATOR OF GAL 4

Question 35

SPECIFICIT OF TRANSCRIPTION DEPENDS ON

Answer 35

PROMOTER AND ACTIVATOR

Question 36

PROXIMAL CONTROL ELEMENTS RESPECTIVELY

Answer 36

GC BOX=MRE=CAAT BOX

Question 37

GC BOX

Answer 37

CONSTITITUIVE BASALA EPXRESSION

Question 38

CAAAT BOX

Answer 38

CBT BINDS TO IT , EFFECIENCE OF THE PROMOTER , BIDIRECTIONAL

Question 39

HYPERINSULINEMIA

Answer 39

AGE AT MENARCHE AND OBESITY
INTAK A LOT - SHBG

Question 40

contraceptives,

Answer 40

داروهای ضد بارداری،

Question 41

fecundity

Answer 41

باروری