LAM3_35-53

Question 1

Name the order, family, subfamily, and genus of the lab mouse.

Answer 1

Order Rodentia, family Muridae, subfamily Murinae, genus Mus.

Question 2

Mice have ___ chromosomes.

Answer 2

40

Question 2

The ______loci control expression of cell surface molecules that modulate major immunological phenomena. This complex is one of the most studied genetic systems of the mouse.

Answer 2

Histocompatability

Question 3

Mating mice of two inbred strains produces what?

Answer 3

F1 hybrid mice

Question 4

Recombinant inbred strains a developed how?

Answer 4

By single-pair random matings of mice from an F2 generation created by crossing mice of two inbred strains.

Question 5

How are transgenic strains named?

Answer 5

Symbol for the strain followed by a symbol for the transgene- e.g. Tg(YYYYY)#Zzz, where Tg = transgene, (YYYYY) = description of the inserted DNA, # = assigned number in the series of events generated using a given construct, Zzz = lab code

Question 6

What are some criteria for rodent contact bedding?

Answer 6

Moisture absorbent, dust free, does not promote microbial growth, atraumatic, sterilizable, uniform batch to batch, nontoxic, inexpensive

Question 7

Adult mice drink ____ml of water per day.

Answer 7

6-7 ml

Question 8

Due to the large surface area per gram of body weight, mice have significant physiologic changes in response to change in ambient temperature. T or F

Answer 8

TRUE

Question 9

The neonatal mouse is ectothermic. T or F

Answer 9

TRUE

Question 10

The neonatal mouse develops temperature control about ____ days of age.

Answer 10

20 days of age

Question 11

The thermoneutral zone (29.6-30.5 C) is the optimum temperature for mice to live. T or F

Answer 11

False- 21-25 C is the idea temperature for mice, not the thermoneutral zone

Question 12

The right lung has ____lobes; the left lung has _____.

Answer 12

Right = 4 lobes; left = 1 lobe

Question 13

Describe the stomach of the mouse.

Answer 13

The proximal portion is keratinized, the distal portion is glandular.

Question 14

The altered Schaedler flora (ASF) are given to mice to make them ___________ (know microbiota).

Answer 14

Gnotobiotic

Question 15

What bacteria are in the “altered Schaedler flora” (ASF)?

Answer 15

Four members of the original Schaedler flora (two lactobacilli, Bacteroides distasonis, and the EOS fusiform bacterium), a spiral-shaped bacterium, and three new fusiform EOS bacteria.

Question 16

The thymus reaches maximum size at ________ and involutes at _________.

Answer 16

sexual maturity, 35-80 days of age.

Question 17

Mean systolic blood pressure is (range)__________ and heart rate is (range)_______.

Answer 17

84-105 mm Hg, 310-840/ min

Question 18

Normal dentition in a mouse is ____ incisor and ____ molars in each quadrant.

Answer 18

1, 3

Question 19

The female mouse has how many pair of mammary glands?

Answer 19

5

Question 20

Sexual maturity occurs in the female at _____ days and the male at ____.

Answer 20

24-28 days, up to 2 weeks later

Question 21

Describe the estrous cycle of the mouse.

Answer 21

Polyestrous, cycles every 4-5 days, spontaneous ovulators

Question 22

Ovulation does not accompany every estrus, and estrus may not coincide with every ovulation. T or F

Answer 22

TRUE

Question 23

What is the Whitten effect?

Answer 23

Estrus is suppressed in mice housed in large groups because of pseudopregnancy or diestrus

Question 24

What is the Bruce effect?

Answer 24

Pheromones from a strange male mouse may prevent implantation or pseudopregnancy in recently bred females.

Question 25

Gestation is ___ days.

Answer 25

19-21

Question 26

There are two categories of pheromones. Name them.

Answer 26

Primer and releaser pheromones

Question 27

There are ____ classes of immunoglobulins.. They are :

Answer 27

5 , IgM, IgD, IgG (subtypes IgG1, IgG2a, IgG2b, IgG3, IgA, IgE

Question 28

Mouse T lymphocytes can be differentiated into 2 phenotypes:

Answer 28

CD4+ T cells (MHC class II) and CD8+ T cells (MHC class I)

Question 30

Definition of Inbred

Answer 30

Strain established by a minimum of 20 consecutivegenerations of sister × brother matings (sib matings)

Question 31

Definition of Outbred

Answer 31

Stock/line established and maintained by strictavoidance of sib mating

Question 32

F1 hybrid

Answer 32

Created by mating mice of two inbred strains in adefined direction

Question 33

F2 hybrid

Answer 33

Created by mating two F1 hybrid mice

Question 34

Recombinantinbred

Answer 34

Lines established by inbreeding of the progeny ofindividual F2 hybrid sibling pairs for at least 20generations

Question 35

Congenic

Answer 35

Introduction of a mutation onto a defined inbredstrain background by 5–10 or more generations ofmatings back to the inbred strain with selection forthe mutation (backcrossing)

Question 36

Consomic

Answer 36

An inbred strain that carries one chromosome fromanother inbred strainTen generations of backcrossing and selection for thedonor chromosome is required for a fully consomicstrain

Question 37

Conplastic

Answer 37

An inbred strain that carries the nuclear genome ofone inbred strain and the cytoplasm and cytoplasmic(mitochondrial) genome of anotherSince the egg contributes the embryonic cytoplasm,10 generations of backcrossing inbred females to aninbred male recipient is required for a fully conplasticstrain

Question 38

RFLP

Answer 38

Restriction fragment length polymorphism - DNA is isolated, digested by bacterial restriction enzymes, then the lengths of the digested fragments compared for differences.

Question 39

SNPs

Answer 39

Single Nucleotide Polymorphisms - identified variations in single nucleotides used to monitor genotypes