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Mice > Jax Handbook - Chap 8 Genetic QC > Flashcards

Jax Handbook - Chap 8 Genetic QC Flashcards

1
Q

Regarding genetic contamination and genetic drift:

        a. Genetic contamination occurs slowly in time b. One strategy to prevent genetic contamination consists on housing adjacently strains which are phenotypically and genotypically different. c. When a escaped mouse is found the recommendation is to make a genetic analysis to know to which strain source it has to be returned. d. Genetic contamination occurs when a colony expansion exceeds 20 generations.
A

b. One strategy to prevent genetic contamination consists on housing adjacently strains which are phenotypically and genotypically different.

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2
Q

Find the FALSE sentence regarding Genetic Drif:

        a. It occurs slowly in time
        b. It is caused by spontaneous mutations that become fixed
        c. It is easy to detect with genetic tools
        d. Strict sister-brother mating can help to reduce Genetic Drift
A

c. It is easy to detect with genetic tools

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3
Q

T/F Strict sister-brother mating minimizes effects of Genetic Drift and maximizes heterozygosity.

A

False

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4
Q

T/F In order to minimize Genetic Drift it is recommended to replenish breeders from the supplier periodically.

A

True

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5
Q

Name the technique through which it is possible to apply a recently developed strategy which minimizes genetic drift and lengthens effective generation time by replenish breeders periodically in a very efficient way?

A

Cryopreservation

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6
Q

Choose the correct: “GENETIC CONTAMINATION//GENETIC DRIFT is extremely difficult to identify”.

A

GENETIC DRIFT

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7
Q

When managing to identify Genetic Drift and Genetic contamination:

a. To detect Genetic Contamination it is necessary to do a screening marking the 90% of chromosomes of a strain.
b. Discovery of just two or more aberrant chromosomes is sufficient evidence to indicate that there is Genetic Contamination.
c. The most recommended method to monitor and identify Genetic Contamination is by observation of the different phenotypes between strains.
d. All the above are false

A

b. Discovery of just two or more aberrant chromosomes is sufficient evidence to indicate that there is Genetic Contamination

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8
Q

T/F When Genetic Contamination is detected in a colony it needs to be culled.

A

True

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9
Q

Regarding Production Colonies of the Jackson Laboratory:

a. Foundation stocks are maintained by pedigreed sister-brother breeding pairs
b. Trio mating is used in foundation stocks for more rapid expansion.
c. Mice in the pedigreed expansion stock are never more than 5 generations removed from the founding pair.
d. a and c are true

A

d. a and c are true

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10
Q

Regarding Foundation Stocks at Jackson Laboratory:

a. Normally 4 to 5 families per strain is maintained.
b. Mice are maintained by crossing pooled breeders between families.
c. The animals are never crossed more than 5 generations away from a single sister-brother breeding pair.
d. Embryos from foundation stocks are cryopreserved in case it is needed to restart the stock

A

d. Embryos from foundation stocks are cryopreserved in case it is needed to restart the stock

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11
Q

T/F Sibling trio mating (two sisters, one brother) is used in Pedigreed expansion stocks in Jackson Laboratory.

A

True

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12
Q

Regarding Production Stocks at Jackson Laboratory:

a. the mice produce are distributed to researchers
b. Pooled breeders from sister-brother and trio mating from Pedigreed expansion stocks are crossed.
c. The crossing never exceeds 2 generations.
d. All the above are true

A

d. All the above are true

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13
Q

T/F The live repository of Jackson Laboratory is maintained as pedigreed foundation stocks.

A

True

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14
Q

Regarding cryopreserved stocks at Jackson Laboratory:

a. Only embrios are preserved
b. Only sperm is preserved
c. frozen material can be directly ship to researchers
d. The main purpose of cryopreserved stocks is to maintain strains with a very low demand

A

c. frozen material can be directly ship to researchers

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15
Q

T/F Repository colonies at Jackson Laboratory breed large quantities of strains

A

False

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16
Q

T/F Research Colonies are not subject to the institutional policies for genetic quality control of JAX® Mice.

A

True

17
Q

Regarding the Genetic Quality Control Program in Jackson Laboratory:

a. A single nucleotide polymorphisms (SNPs) genotyping is used to control the stability of the different strains.
b. In the pedigreed expansion stock each breeder pair is genotyped before setting them up.
c. For all Pedigreed expansion stocks 20 mice are genotyped annually
d. In production Stocks every month six mice of the most popular 100 strains is genotyped.

A

a. A single nucleotide polymorphisms (SNPs) genotyping is used to control the stability of the different strains.

18
Q

Find the FALSE sentence: regarding genetic monitoring of Repository Colonies at the Jackson Laboratory:

a. all new mutant or genetically engineered mice is characterized
b. Every sister-brother breeding pair in the foundation stock is evaluated to check the presence of the mutant allele.
c. Every two months each strain is evaluated through SNP genotyping for possible contamination
d. periodically, expected phenotypes of selected genetically engineered and mutant mice is verified

A

c. Every two months each strain is evaluated through SNP genotyping for possible contamination

19
Q

T/F In the Jackson Laboratory, upon suspicion of contamination through visual monitoring by technicians the animal and its offspring is immediately culled

A

False

If genetic contamination is CONFIRMED, it is our strict policy to notify anyone who purchased
the mice as quickly as possible and to cull the colony as appropriate. Then we re-establish the
colony, genotyping new mice to ensure their genetic integrity.

20
Q

T/F The Genetic Stability Program of the Jackson Laboratory allows to re-establish foundation stocks and it extends the length of a single generation to between 25 and 50 years.

A

False

For each foundation stock in the program Jax
freezes enough embryos from a single generation to last 10–25 years. Every five generations, they
re-establish foundation stocks from the frozen embryos.

Mice (20 decks)

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