BB Ch27 - Genetic Monitoring Flashcards
- The first (oldest) inbred mouse strain was ___ and was
described in ____ ?
DBA, 1909
- What does “Genetically defined” mean?
Genetic homogeneity between individual animals of a breeding
group.
4. Which of the following fit the definition of “genetically-defined”? a. F1 hybrids (between inbred strains) b. Outbred c. Inbred and outbred d. Random-bred, inbred
a
Many inbred strains were initially developed to study the role
of genes in ?
cancer
What is a common chemical agent used to generate mice carrying
point mutations?
ENU
At F20 of brother-sister matings, the number of loci that remained unfixed is estimated
to be?
273
What is one of the cardinal indicators of genetic problems in
a colony?
Improved reproductive performance
Congenic strains .
a. Do not need to be genetically monitored at each back cross
generation.
b. Are created by complete back crossing to the recipient strain
usually 10-12 generations.
c. Can be made faster if the animal with the least residual
homozygosity is chosen for the breeder in the subsequent
generation.
d. Are rare because to take over 3 years to produce them.
b
The majority of mutations remain silent, mutations that occur
in ____ and ______ , may alter physiology of a given strain.
Coding and regulatory regions
What is the most common source of genetic variation within
rodent colonies?________
human error
“Speed congenics” are made using _____ markers.
PCR amplified SSLP or SNPs
Tail skin grafting should be observed for _____ before drawing conclusions.
100 days
Name three immunologic assays used to detect genetic
differences among strains of rodents.
microcytotoxic assay, Hemagglutination assays, Mixed
lymphocyte reaction.
In the pyramid breeding system the top of the pyramid is?
foundation stock
Tail skin grafting is an easy way to check strain purity.
True
