Jax Handbook - Chap 9 Animal Husbandry

Question 1

T/F Some animal husbandry tasks are simple and repetitive but this does not imply that they are easy to do well.

Answer 1

True

Question 2

What is the objective of husbandry tasks?

Answer 2

To provide a calm and comfortable environment for the animals so sthey remain healthy and free from stress as much as possible

Question 3

Name Three tradeoffs that influence the choice of mice cage system

Answer 3

Cost, convenience and effectiveness

Question 4

Name four functions of cage systems

Answer 4

Housing, feeding, watering, room organization, disease protection

Question 5

What are the three types of mice cage systems?

Answer 5

Conventional cages

Microisolators

IVC

Question 6

Which cage system is…
Less expensive?
That gives more protection?
With less air circulation?
That needs more frequent cage change?

Answer 6

Conventional

IVC

Microisolator

Convetional

Question 7

What does HEPA stand for?

Answer 7

High Efficiency Particulate Air Filter

Question 8

In top covered filter cages, poor air circulation can affect intra cage…

a. temperature
b. ammonia level
c. humidity
d. all of above

Answer 8

d. all of above

Question 9

T/F Having mice housed in microisolators or IVCs means work with mice can be done on open table

Answer 9

False

Question 10

T/F In conventional cages, air circulation is determined exclusively by room air circulation

Answer 10

False

Air circulation in cages is determined
by room circulation and by air flow
caused by the thermal heat load of the
mice pg 202

Question 11

What are the two factors that influence air circulation in conventional cages?

Answer 11

Air circulation in conventional open top cages is determined by room circulation and by air flow
caused by the thermal heat load of the
mice pg 202

Question 12

What cage system protect mice from pathogens and also personnel from allergens?

Answer 12

IVC

Question 13

What cage system allows reduce change frequency? Why?

Answer 13

IVC

Cages remain dry, which may
reduce cage change frequency and
labor expense.

Question 14

T/F Cages made with polyphenyl-sulfone are highly recommended if they will be exposed to quaternary ammonium cleaners

Answer 14

True

Caging made of polyphenyl-sulfone or other chemically resistant material is highly recommended if there is any
possibility of exposure to quaternary ammonium cleaners or other high pH agents pg 202

Question 15

T/F Ventilation in individually ventilated cages can result in drafts inside it, which can induce stress in mice

Answer 15

True

Question 16

T/F When caging mice of different size, floor space assigned to all is related to the one assigned for the largest mouse.

Answer 16

True

Question 17

What are the two main systems to watering mice?

Answer 17

Automatic & bottle based

Question 18

Name three disadvantages of automatic watering system

Answer 18

• It requires a capital expense.
• System must be maintained (per
manufacturer’s instructions) to prevent
buildup of mold and bacteria.
• Some mice may need “training” to learn
how to use an automatic system.
• It is difficult to determine water usage
for specific cages.
• Leakage in some systems may cause
mice to drown if cages fill with water.
• Cages must be checked daily

Question 19

Name two advantages of bottle –based watering system from the automatic one

Answer 19

• Water usage per cage can
be easily monitored.
• Sterilization of bottles and
lids is very
straightforward; pathogen
protection is high.
• Treatments can be easily
provided in the water on a
cage-specific basis.

Question 20

T/F Plastic bags filled with water can be stored for longterm and are a viable option to include in an emergency plan

Answer 20

True

Question 21

T/F Light period recommended for both maintenance and breeding are 12:12 L:D

Answer 21

False

Normal:12:12 L:D
Breeding: 14:10 L:D

Question 22

Recommendation of room air changes per hour are

a. 5 - 10
b. 10 – 15
c. 15 – 20
d. 20 – 25

Answer 22

b. 10 – 15

Question 23

Recommendation for relative humidity in room environment is

a. 20-25%
b. 10-40%
c. 30-70%
d. 50-90%

Answer 23

c. 30-70%

Question 24

What are the recommended ranges (Guide) for the following environmental conditions for mice?

Temp

Relative humidity

Air flow

Light period

Light intensity

Noise

Answer 24

Temp = 20-26C (28-79F)

Relative humidity = 30-70%

Air flow = 10-15 fresh air changes per hour

Light period = timing should ensure a regular diurnal cycle

Light intensity = 130-325 lux at cage level

Noise = sounds > 85 dB are associated with auditory and non auditory effects

Question 25

T/F A light cycle of 12:12 L:D is recommended for breeding, whereas 14:10 L:D is recommended for maintenance

Answer 25

False 12: 12 L:D for maintenance 14: 10 L:D for breeding

Question 26

What level of noise can induce seizures in certain mice strains? a. 75 dBA b. 85 dBA c. 95 dBA d. 105 dBA

Answer 26

c. 95 dBA

Question 27

Light intensity standard for worker safety is... a. 10 foot candles at 1 meter from floor b. 20 foot candles at 1 meter from floor c. 30 foot candles at 1 meter from floor d. 40 foot candles at 1 meter from floor

Answer 27

a. 10 foot candles at 1 meter from floo

Question 28

T/F Mice prefer low light levels rather than higher ones

Answer 28

True

Question 29

Temperature recommendations for mice room are... a. 17-26ºC b. 19-28ºC c. 21-30ºC d. 23-32ºC

Answer 29

a. 17-26ºC

Question 30

What are the three functions of bedding?

Answer 30

Provides warmth, absorbing moisture, environmental enrichment

Question 31

T/F Bedding has no influence in bacterial microenvironment

Answer 31

False Bedding affects the bacterial microenvironment and thus affects the rate at which bacteria break down urea to produce ammonia in the cage.

Question 32

T/F Bacteria produce ammonia from urea in the age at same rate independently of the type of bedding

Answer 32

False Bedding affects the bacterial microenvironment and thus affects the rate at which bacteria break down urea to produce ammonia in the cage.

Question 33

T/F Pellets of corn cob and hardwood shavings are physiologically inert for mice.

Answer 33

True No changes produced in p450 enzymes

Question 34

What type of bedding is not good for nesting? a. Recycled paper b. cedar softwood c. pellets of corn cob d. cotton material

Answer 34

c. pellets of corn cob additional nesting material may be required.

Question 35

Which one of the next bedding material is least absorbent? a. softwood shaving b. pellets of corn cob c. cotton material d. cellulose chips

Answer 35

b. pellets of corn cob

Question 36

Which one of the next bedding material is most absorbent? a. recycled paper b. cellulose chips c. cotton material d. hardwood shavings

Answer 36

a. recycled paper: provides superior absorbency and ammonia control c. cotton material: is highly absorbent and is good for ammonia control when shredded

Question 37

T/F Recycled paper as bedding may be irritating for nude mice because they no have eyelashes

Answer 37

True

Question 38

Which bedding type is not considered physiologically inert because they induce cytochrome p450 enzymes?

Answer 38

Cedar shavings elevate expression of hepatic p450 enzyme systems

Question 39

Day to day care for mice involves: a. protect them from pathogens b. keep them feed and watered c. maintain their genetic integrity d. all of above

Answer 39

d. all of above

Question 40

Name four ways of pathogen entry in mice colonies

Answer 40

new mice from other colonies humans supplies air

Question 41

T/F Procedures to protect personnel from mice allergens are different from those to protect mice from pathogen

Answer 41

False Procedures that protect animals from human-borne pathogens also protect technicians from animalborne allergens.

Question 42

What is the general recommendation for personnel who have to work in different mouse colonies during the same day?

Answer 42

If technicians or researchers must visit multiple mouse rooms in a single day, they must start in the room with the highest level of pathogen protection and work their way down to the rooms with lower levels.

Question 43

T/F Signs in mice that might indicate illness include non-responsiveness to routine cage disturbance and unusual fighting

Answer 43

True

Question 44

T/F The level protection provided when investigators are working with their mice must be at least equal to that of the cages in which they are housed. This same standard need not be applied during cage change.

Answer 44

False Procedures for changing cages involve distribution of clean cages, food and water, removal of used and dirty cages, cage contents, and equipment, all performed under requirements that meet or exceed the level of pathogen protection for any single room.

Question 45

What is the frequency recommended for changing out conventional cages? a. weekly b. biweekly c. twice a week d. as needed

Answer 45

a. weekly

Question 46

Name the two procedures that can be used to transfer mice between cages.

Answer 46

Gloved hand Dressing forceps

Question 47

What is the recommended minimum age of mice to be transfer by forceps? a. 10 days b. 15 days c. 20 days d. 30 days

Answer 47

b. 15 days

Question 48

T/F To prevent pathogen spread from cage to cage, it is recommended to sanitize forceps or gloves between them

Answer 48

True

Question 49

To prevent fights while changing male mice... a. randomize them from different cages b. change cage monthly c. place a small lump of dirty shavings in the new cage d. all of above

Answer 49

c. place a small lump of dirty shavings in the new cage

Question 50

What is the best way to avoid mistakes when perform cage changing?

Answer 50

Work with a detailed standard operating procedure and train personnel adequately

Question 51

T/F To avoid escapes during cage changing, never have two cages open at the same time

Answer 51

True

Question 52

To ensure that food and water are not entry points for pathogens into the mouse room, food should be: a. pasteurized b. sterilized c. irradiated d. all of above

Answer 52

d. all of above

Question 53

How much do “normal” mice eat and drink each day?

Answer 53

Per 10 g of body weight: mice fed ad libitum consume approximately 1.5 g of dry pelleted food per day and drink 1.5 ml of water per day.

Question 54

Which of the following may be used to minimize genetic contamination... a. house different strains in different rooms b. if strains sharing same room it is better they have different coat colors c. keep meticulous records d. all of above

Answer 54

d. all of above

Question 55

Which of the following is the most sensitive indicator of stress in mice? a. stereotypic behavior b. dominance beahviour c. reproductive performance d. barbering

Answer 55

c. reproductive performance

Question 56

Many strains of laboratory mice are blind and/or deaf by what age? a. 4 month old b. 8 month old c. 12 months old d. 16 month old

Answer 56

b. 8 month old

Question 57

Probably the greatest single source of enrichment to a mouse is…

Answer 57

another mouse

Question 58

T/F: Aggression appears to be more common among males of specific strains (for example, SJL/J and most BALB/c strains) and is much more likely to occur among unrelated males who are first caged together after they are sexually mature.

Answer 58

True

Question 59

T/F: When group-housing males who have not previously lived together, use a clean cage, and try not to transfer any scents from any of the used cages to the new one.

Answer 59

True

Question 60

T/T: Wild-derived inbred mice, which were trapped in the wild and inbred without domestication, generally are less sensitive than common inbred strains to stresses associated with shipping, new surroundings, and new handlers.

Answer 60

False These mice are typically MORE sensitive to stresses

Question 61

T/F: Aggression is particularly prevalent among mice of the CZECHII/Ei strain and in progeny of any crosses involving Mus musculus castaneus.

Answer 61

True