Laboratory: Proteins Flashcards

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1
Q

a naturally occurring, unbranched polymer in which the monomer units are amino acids

A

PROTEINS

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2
Q

a peptide in which at least 40 amino acids residues are present

A

PROTEINS

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2
Q

a peptide in which at least 40 amino acids residues are present

A

PROTEINS

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3
Q

Proteins is from the Greek word proteios, meaning ___________

A

preeminence

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4
Q

The most important of all biological substances

A

PROTEINS

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5
Q

List down the protein sources

A
  1. Milk
  2. Wheat flour
  3. Beans
  4. Minced beef
  5. Egg
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6
Q

the most nutritionally complete food found in nature

A

MILK

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7
Q

WHOLE MILK vs SKIM/SKIMMED MILK:

is an oil-in-water emulsion, containing its 3.9% fat dispersed as micron sized globules

A

WHOLE MILK

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8
Q

WHOLE MILK vs SKIM/SKIMMED MILK:

is made when all the cream (also called milkfat) is removed from whole milk.

A

SKIM/ SKIMMED MILK

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9
Q

WHOLE MILK vs SKIM/SKIMMED MILK:

It tends to contain around 0.1% fat

A

SKIM/ SKIMMED MILK

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10
Q

main protein in milk

A

Casein

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11
Q

is a phosphoprotein which has phosphate groups attached to the hydroxyl groups of some of the amino acids side-chain

A

Casein

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12
Q

a mixture of three similar proteins, alpha, beta and kappa caseins which form a micelle

A

Casein

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13
Q

__________ are both insoluble in water and are solubilized by the micelle surrounding them

A

Alpha- and beta-casein

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14
Q

has a hydrophilic portion is responsible for solubilizing the other two caseins by promoting the formation of and stabilizing the micelles.

A

kappa-casein

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15
Q

responsible for the white opaque appearance of milk

A

micelles

16
Q

has an isoelectric point of pH 4.6

A

Calcium caseinate

17
Q

What is the pH of milk?

A

6.6

18
Q

Is the pH at which an amino acid solution has no net charge because an equal number of positive and negative charges are present.

A

ISOELECTRIC POINT

19
Q

That pH value is known as the ________ of the protein and is generally the pH at which the protein is least soluble.

A

isoelectric point (IEP)

20
Q

At this point, almost all amino acid molecules in a solution are present at their zwitterion form.

A

ISOELECTRIC POINT

21
Q

is a mixture of two proteins, glutenin and gliadin

A

GLUTEN

22
Q

It is also the composite of a prolamin and glutelin, which exist, conjoined with starch, in the endosperm of various grass-related grains.

A

GLUTEN

23
Q

is a protein composite found in wheat and related grains, including barley and rye.

A

GLUTEN

24
Q

_____ gives elasticity to dough, helping it rise and keep its shape and often gives the final product a chewy texture

A

Gluten

25
Q

The principle involved in the isolation of gluten is difference in solubility. The starch is ________ in water while gluten is _____ in water.

A

partially soluble, insoluble

26
Q

What solution is used to test the complete removal of starch, which involves the formation of blue-iodo starch complex?

A

Iodine solution

27
Q

What keeps the protein from degrading?

A

Refrigeration

28
Q

Detection of Nitrogen:

What color indicates no presence of Nitrogen or ammonia?

A

Red-red

29
Q

Detection of Nitrogen:

What color indicates the presence of ammonia (NH3)gas & urine like odor (presence of Nitrogen)

A

Red-Blue

30
Q

What color precipitation is there in the Detection of Sulfur?

A

White ppt. (Barium Sulfate BaSO4)

31
Q

What color precipitation is there in the Detection of Phosphorus?

A

Yellow ppt. (NH4)3PO4.12MoO3 ammonium phosphomolybdate

32
Q

Most of these reactions are catalyzed by ______so that they can proceed at the rate necessary to sustain life.

A

enzymes

33
Q

Enzymes are ______ found in all living organisms.

A

proteins

34
Q

In an overall reaction, ______ are not consumed but are converted back into their natural state after a reaction

A

enzymes

35
Q

reactive site where molecular reaction takes place

A

Enzymes

36
Q

are molecules that bind in the active site of an enzyme

A

Substrates