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BIO 024: Biochemistry > Basic Lab Techniques > Flashcards

Basic Lab Techniques Flashcards

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1
Q

List 12 Laboratory Techniques

A
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2
Q

A rugged, inexpensive weighing instrument used in measuring mass. It is named for the three beam which carry weights.

A

Triple Beam Balance

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3
Q

Used for preparative work where an error of ±0.02 g is acceptable.

A

Top Load Balance

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4
Q

highly sensitive lab instruments designed to accurately measure mass. Their readability has a range between 0.1mg - 0.01mg.

A

Analytical Balance

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5
Q

List all the steps in transferring liquids using pipettes.

A
  1. Using chemical suction
  2. Wipe off outside of pipette with gauze
  3. Adjusting the meniscus
  4. Drain into receiving vessel
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6
Q

process where liquid is converted to a gaseous state through wind and air temperature changes

A

Evaporation

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7
Q

technique used to separate solids from liquids, is the act of pouring a mixture onto a membrane (filter paper) that allows the passage of liquid (the filtrate) and results in the collection of the solid.

A

Filtration

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8
Q

process that can be used to separate a pure liquid from a mixture of liquids. It works when the liquids have different boiling point

A

Distillation

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9
Q

This refers to heating a solution with an attached condenser to prevent reagents from escaping. “ to increase the rate of chemical reaction”

A

Reflux Heating

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10
Q

used in the identification of certain metals. It is based on the observation that light emitted by any element gives a unique spectrum

A

Flame Test

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11
Q

Which of the following apparatus is not included in the distillation setup?

  • Hot plate
  • Condenser
  • Adapter
  • Thermometer
  • Test tube
A

Hot plate (not sure)

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12
Q

List all the basic laboratory technique

A
  1. Measuring mass
  2. Measuring volume
  3. Heating and evaporation
  4. Distillation
  5. Filtration
  6. Reflux heating
  7. Flame test
  8. Sublimation
  9. Decantation
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13
Q

solid to gas

A

Sublimation

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14
Q

the process of separation of liquid from solid and other immiscible (non-mixing) liquids, by removing the liquid layer at the top from the layer of solid or liquid below

A

Decantation

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14
Q

also known as a sample tube, is a common piece of laboratory glassware consisting of a finger-like length of glass or clear plastic tubing, open at the top and closed at the bottom

A

Test tube

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15
Q

laboratory equipment used to hold upright multiple test tubes at the same time

A

Test tube rack/stand

16
Q

is a brush used for cleaning test tubes and narrow mouth laboratory glassware, such as graduated cylinders, burettes, and Erlenmeyer flasks

A

Test tube brush/spout brush

17
Q

used to hold test tubes. It is used for holding a test tube in place when the tube is hot or should not be touched

A

Test tube holder

18
Q

Used as containers, is available in a variety of sizes. Although they possess volume markings, these are only rough estimates of the liquid volume. The markings are not necessarily accurate.

A

Beakers

19
Q

Often used as reaction vessels, particularly in titrations. As with beakers, the volume marking should not be considered accurate.

A

Erlenmeyer Flask

20
Q

Used to measure and store solutions with a high degree of accuracy.

A

Volumetric Flask

21
Q

Used to transfer liquids with a moderate degree of accuracy.

A

Graduated Cylinder

22
Q

Used for transferring liquids with a fixed volume and quantity of liquid must be known to a high degree of accuracy.

A

Pipette

23
Q

These are made of plastic and are useful for transferring liquids dropwise.

A

Disposable Pipette

24
Q

A tube or pipe that is wide at the top and narrow at the bottom, used for guiding or powder into a small opening.

A

Funnel

25
Q

It is a small dish shaped like a cylinder.

A

Petri Dish

26
Q

Used to mix chemicals and liquids for laboratory purposes. They are usually made of solid glass, about the thickness and slightly longer than a drinking straw, with rounded ends.

A

Glass/Stirring Rod

27
Q

It is a semi-permeable paper barrier placed perpendicular to a liquid or air flow. It is used to separate fine substances from liquids or air. It is used in science labs to remove solids from liquids.

A

Filter paper

28
Q

A circular concave piece of glass used in chemistry as a surface to evaporate a liquid, to hold solids while being weighed, for heating a small amount of substance and as a cover for a beaker.

A

Watch Glass

29
Q

Used to prepare ingredients or substances by crushing and grinding them into a fine paste or powder in the laboratory.

A

Mortar and Pestle

30
Q

It contains a chemical that changes color as it makes contact with an acid or base. The paper will turn red in acids and blue in bases.

A

Litmus and pH paper

31
Q

Always add acid to _______.

A

Water

32
Q

What is the proper measurement of the volume of a colorless liquid?

  • Above the meniscus
  • Below the meniscus
  • At the center of the meniscus
A

At the center of the meniscus

33
Q

What are the two physical changes involved in the distillation setup?

A

Evaporation and condensation

34
Q

What is the liquid you obtained after filtration setup

A

Residue

35
Q

At what part of the Bunsen burner where the amount of air that enters is controlled?

A

Collar

35
Q

What is important to remember while you are using heat?

  • Do not heat closed containers
  • Point the mouth of the container away from yourself and classmates.
  • Always use clamps to hold the container.
  • All of the above
A

All of the above

36
Q

True or False:

Observing proper and correct laboratory techniques is important in maintaining safety and attaining accurate results

A

True

BIO 024: Biochemistry (16 decks)

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