Laboratory Mice Flashcards
What are the advantages of using mice in research?
Small Low cost Biology is well understood Can be genetically manipulated Short life span = studies end sooner
What is the common name of Mus musculus
Swiss Albino mouse or House mouse
What is the common name of Peromyscus?
Deer or cotton mouse
What is the common name of Mus musculus musculus?
Eastern Europe Mouse
What is the common name of Mus musculus domesticus?
Western Europe mouse
What is the common name of Mus musculus castaneus?
Southeast Asia mouse
What is the common name of the Mus musculus molossinus?
Japan mouse
What is the life span of a mouse?
12-36 months
What is the age of puberty?
5-8 weeks
What is the length of the estrus cycle?
4-5 days
What is the length of gestation of mice?
19-21 days
What is the average litter size of mice?
7-11 (4-12+)
What is the weaning age of mice?
18-21 days
What gland do mice have that rats also possess?
Harderian gland
Where do mice have a high venous blood supply?
Orbital sinus
Mice are Coprophagic, which means they need to eat their feces to recover what vitamin?
B vitamin complex
Can mice vomit?
No.
How many pairs of mammary glands do mice have?
5
How many digits do mice have on their forelimb?
4
How many digits do mice have on their hind limbs?
5
What effect describes Housing female mice in groups will result in synchronization of their estrus cycles. Prolonged absence of male pheromones results in a state of anestrus (lack of a normal estrus cycle).
Lee Boot Effect
What allows for timed mating of large group of females when females are housed together without a male and then begin estrus within 3 days when exposed to a male or the odor of a male?
Whitten Effect
What effect describes If a pregnant female mouse is exposed to a new male or its odor within 4 days of breeding, the existing pregnancy will usually be aborted and the female will return to estrus.
Bruce Effect
What term describes The developmental arrest induced by environmental conditions related to the survival of the dam and her ability to nourish developing embryos. * Females that are bred postpartum estrus have an extended gestation period. *
Facultative Diapause
What are the young called?
Pups
What are breeding females called?
Moms
What do the pups look like by 1 week of age?
Hair has grown in
What do pups look like at 2 weeks of age?
Eyes are open
Can see eyes open as early as 12 days
How old are the pups when moist food can be given so they can begin weaning
15 days
Hybrid
2 inbred strains bred
Outbred
Stock
Randomly bred
KO
Genetically engineered
One or more genes have been turned off through a targeted mutation
Inbred
Homozygous Strain
20 generations
Transgenic
Removing DNA sequences and inserting them into an ovum
Stocks and Strains Outbred and Inbred: Swiss Webster
Albino
Outbred
Stocks and Strains Outbred: CD-1
Albino
Outbred
Stocks and Strains Inbred: C57-BL
Black
Inbred
Stocks and Strains Inbred: DBA/2
Gray
Inbred
Stocks and Strains Inbred: Balb/C
Albino
Inbred
Stocks and Strains Inbred: SCID
Albino
Severe combined immunodeficient
Stocks and Strains Inbred: Nu/nu
Athymic nude mice
SCID mice
Severely compromised immuno deficient
Lack B & T cells
Nude Mice
Lacks B cells
Lack Thymus gland
What is the ideal temperature mice should be kept in?
65 to 85 degrees F
What is the ideal humidity mice should be kept in?
40% to 60%
How much water do mice need to consume per day?
15mL per 100g of body weight
How much food do mice need to consume per day?
12-18g of food per 100g of body weight
Why should you never pick a mouse up by grasping the middle or tip of the tail?
The scales can slough off result in a severe de-gloving injury
What percentage of blood volume should not be removed at one sampling?
10%
What is the blood volume of a mouse?
8% of the body weight
