LAB2 - MANUAL COUNT FOR PLTS

Question 1

the indirect method of counting platelets

Answer 1

well-prepared slides and counted using microscope

Question 2

the direct method of counting platelets

Answer 2

the use of rbc pipette and hemocytometer

Question 3

__ are the smallest formed elements in the
blood,

Answer 3

Platelets

Question 4

size of platelets

Answer 4

2-4 um

Question 5

due to Small size platelets are often hard to differentiate from___ and ___

Answer 5

bacteria and debris

Question 6

Platelets are difficult to count due to:

Answer 6

Small size
Adhesiveness- adhere to rbc pipette
Aggregation

Question 7

___ → diluting fluid; only platelets
will be seen

Answer 7

Rees-Ecker

Question 8

Principle of platelet count

Answer 8

A well-made smear is stained with Wright’s
or Wright’s-Giemsa to visualize cellular elements of

Question 9

___ → use this to
rinse to prevent adherence

Answer 9

Rees and Ecker

Question 10

3 units found in rbc/wbc pipette

Answer 10

0.5, 1.0. 101/11 mark

Question 11

1. The capillary bore of the RBC pipette is rinsed with ____fluid. All excess fluid should be
   removed from the pipette before blood is drawn into the pipette.

Answer 11

Rees-Ecker diluting

Question 12

This step will ensure platelets will not adhere on the wall of the capillary bore of the pipette.

Answer 12

rinsing of rees-ecker diluting fluid before aspirating blood

Question 13

2. Draw the blood into the pipette up to 0.5 mark. If excess blood was drawn, adjust the blood level to the exact 0.5 mark with ____. Clean the stem of the pipette first before any adjustment will
   be made

Answer 13

NSS moistened cotton

Question 14

3. The blood should be diluted immediately with the Rees-Ecker solution. Make a ____dilution by filling the pipette with the solution up to 101 mark. Two pipettes should be used simultaneously, and each should be used to charge one side of the counting
   chamber.

Answer 14

1:200

Question 14

mixing the blood and the rees ecker immediately will prevent the __ of platelets

Answer 14

clumping

Question 14

4. After the dilution, the pipettes should be shaken immediately for at least how many seconds. This step will prevent platelet clumping and will ensure accurate counting.

Answer 14

60 seconds.

Question 14

lying the hemocytometer after charging on wet gauze or cotton pads will prevent what?

Answer 14

evaporation

Question 14

If 25 RBC squares (central large square) are counted area
should be __

Answer 14

1 mm².

Question 14

5. After mixing, discard the first how many drops from each pipette

Answer 14

5 drops

Question 15

6. After charging the chambers, the hemocytometer should be kept in a covered container for about ___ minutes with wet gauze or cotton pads beneath the
   hemocytometer to prevent evaporation, this step will allow the platelets to settle aid in accurate counting later on.

Answer 15

10 to15

Question 16

formula for platelet concentration

Answer 16

𝑜𝑓 𝑐𝑒𝑙𝑙𝑠 𝑐𝑜𝑢𝑛𝑡𝑒𝑑 × 𝑑𝑖𝑙𝑢𝑡𝑖𝑜𝑛 𝑓𝑎𝑐𝑡𝑜𝑟 (200)

divide them all by

𝑎𝑟𝑒𝑎 (1𝑚𝑚^2) × 𝑑𝑒𝑝𝑡ℎ 𝑜𝑓 𝑐𝑜𝑢𝑛𝑡𝑖𝑛𝑔 𝑐ℎ𝑎𝑚𝑏𝑒𝑟 (0.10)

= 𝑝𝑙𝑎𝑡𝑒𝑙𝑒𝑡 × 10^9/L

Question 16

what can we check in indirect method of counting platelet

Answer 16

morphology of the platelet using smear

Question 16

If 5 RBC squares are counted area should be

Answer 16

0.20 mm²

Question 17

___consecutive fields are examined for platelets
and reported as cells per microliter of blood.

Answer 17

10 OIO

Question 17

Principle of indirect method of counting platelet

Answer 17

A well-made smear is stained with Wright’s
or Wright’s-Giemsa to visualize cellular elements blood including the platelet is done in the portion of the smear where the red cells start to overlap

Question 18

conditions where giant platelets are predominant

Answer 18

gene mutation of MYH9 gene
Epstein syndrome

Question 19

please define thrombocythemia

Answer 19

high number of platelet but non functional

Question 20

neutrophil surrounded by platelets caused by EDTA or the sample itself

Answer 20

Platelet satellitism (PS)

Question 21

Tiny platelets (microthrombocytes) → in a
thrombocytopenic patient with what syndrome

Answer 21

Wiskott-Aldrich syndrome

Question 22

Wiskott-Aldrich syndrome

WAS-TER

characteristic triad

Answer 22

T - thrombocytopenia
E - eczema
R - RECURRENT infection

Question 23

Giant bizarre platelet with cytoplasmic
vacuolization in a patient with ___

Answer 23

myelodysplastic syndrome

Question 24

Large platelet in a patient with __

Answer 24

Bernard-Soulier syndrome

Question 25

aside from large platelet in Bernard-Soulier syndrome, what seems to be the other problem?

Answer 25

low number of platelets

Question 26

__ platelet exceeding the size of
background RBCs in a patient with May-Hegglin anomaly

Answer 26

Giant adendritic

Question 27

Usually ___ platelets per OIF represent a normal platelet count

Answer 27

7-20

Question 28

formula for indirect counting

Answer 28

�𝑚𝑏𝑒𝑟 𝑜𝑓 𝑝𝑙𝑎𝑡𝑒𝑙𝑒𝑡𝑠 𝑐𝑜𝑢𝑛𝑡𝑒𝑑 𝑖𝑛 10 𝑜𝑖𝑙 𝑖𝑚𝑚𝑒𝑟𝑠𝑖𝑜𝑛 𝑓𝑖𝑒𝑙𝑑 × 20,000

divide them all in 10

Question 29

PLATELET ESTIMATES REPORT

0-49,000/ul

Answer 29

markedly decrease

Question 30

PLATELET ESTIMATES REPORT

50, 000-99,000/ul

Answer 30

moderately decreased

Question 31

PLATELET ESTIMATES REPORT

100,000 - 149, 000/ul

Answer 31

slightly decrease

Question 32

PLATELET ESTIMATES REPORT

150,000 - 199,000/ul

Answer 32

low normal

Question 33

PLATELET ESTIMATES REPORT

200,000 - 400,000 /ul

Answer 33

normal

Question 34

PLATELET ESTIMATES REPORT

401,000 - 599,000/ul

Answer 34

slightly increased

Question 35

PLATELET ESTIMATES REPORT

600,000 - 800,00/ul

Answer 35

moderately increase

Question 36

PLATELET ESTIMATES REPORT

above 800,000/ul

Answer 36

markedly increased