LAB1 - diff count Flashcards
The ___ determines the
number of each type of white blood cell, present in
the blood.
white blood cell differential count
Materials needed for diff count
- Cell counter
- Glass slides
- Microscope
- Cedar wood oil
- Spreader slides
- Wright’s stain
staining order
stain1 - fixative
stain 2 - eosin stain
stain 3 - new methylene blue
Segmented neutrophil diameter
9-15 um
granules of Segmented
neutrophil
lilac pink granules
how many nucleus does Segmented
neutrophil have?
2-5 lobes
Eosinophil diameter
9-15 um
Eosinophil cytoplasm
reddish
orange granules
Eosinophil lobes
2 lobes
Basophil diameter
10-16 um
Basophil granules
bluish-black granules
Basophil lobes
3-4 lobes
Lymphocytes diamter
8-10 um
Lymphocytes cytoplasm
sky blue
Lymphocytes nucleus
round and compact
Monocytes diameter
15-20 um
Monocytes cytoplasm
blue-gray
Monocytes nucleus
nucleus may
be round, oval, or
kidney shaped, but
more frequently is
deeply indented
(horseshoe shaped) or
folded on itself
ref range of Neutrophil
37-77%
ref range of bad neutrophil
0-11%
ref range of lymphocytes
10-44%
ref range of monocyte
2-10%
ref range of eosinophil
0-7%
ref range of basophil
0-1%