Lab Techniques

Question 1

PCR is used to

Answer 1

Make billions of copies of a DNA Sample

Question 2

PCR reads DNA that goes in _________ direction

Answer 2

5 to 3

Question 3

Diseases that can be diagnosed with PCR

Answer 3

HIV, tuberculosis, Herpes, Leukemias

Question 4

What components does PCR use?

Answer 4

1. Synthetic DNA Polymerase
2. DNA primers

Question 5

Southern Blot is used to

Answer 5

Detect specifique DNA sequence in a sample

Question 6

Northern Blot is used to

Answer 6

Detect specifique RNA sequence in a sample

Question 7

Western Blot is used to

Answer 7

Detects proteins in a sample.

Question 8

What does Western Blot use to detect specific proteins

Answer 8

ANTIBODIES

Question 9

What does Flow citometry identify in a sample

Answer 9

Chemical and physical characteristics of a cell

Question 10

One of the main function of Flow citometry is

Answer 10

Detect for the presence of Specific proteins on the SURFACE of the cell (Identifies Types of Lymphocytes)

Question 11

Flow citometry and HIV

Answer 11

Can be used to measure CD 4 levels

Question 12

ELISA is used for

Answer 12

Detect presence of specific PROTEINS, ANTIGENS or ANTIBODIES in a sample. Using ANTIBODIES specific to the protein

Question 13

What diseases can be diagnosed with ELISA

Answer 13

HIV, Dengue, Malaria, Hepatitis