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[LAB] LDH Flashcards

1
Q

REFERENCE METHOD OF LDH

A

KINETIC METHOD BY BUHL AND JACKSON
MODIFIED BY WACKER

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2
Q

MAJOR ORGANS WHERE LDH IS DISTRIBUTED IN

A

HEART
LIVER
MUSCLE
KIDNEY

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3
Q

ELEVATED LEVELS OF SERUM LDH IS FOUND IN WHAT CLINICAL CONIDITONS

A

MI
LIVER DISEASE
RENAL DISEASE
ANEMIA
MALIGNANT DISEASES
PROGRESSIVE MUSCLE DYSTROPHY

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4
Q

IN WHAT DIRECTION CAN THE LD ENZYME BE MEASURED

A

FORWARD
REVERSE

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5
Q

[ADVANTAGES]
LACTATE TO PYRUVATE METHOD

A

BETTER REAGENT STABILITY
ELIMINATION OF PRE-INCUBATION
LINEAR RATE OF REACTION
LESS CONTAMINATION OF NAD

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6
Q

PRINCIPLE OF LDH

A

L-LACTATE + NAD
<—LDH (PH 8.3 TO 9.0) —>
PYRUVATE + NADH + H

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7
Q

COENZYME OF LDH

A

NAD

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8
Q

DOES LDH ACT ON D-LACTATE

A

NO

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9
Q

WHAT HAPPENS TO PYRUVATE AFTER FORMATION

A

PROCEED TO THE EMBDEN-MEYERHOFF PATHWAY
FOR THE GENERATION OF ENERGY

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10
Q

SOLUTIONS MIXED WITH NADH IN THE WACKER COLORIMETRIC METHOD

A

PHENAZINE METHOSULFATE
NITROBLUE TETRAZOLIUM

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11
Q

END PRODUCT OF THE WACKER COLORIMETRIC METHOD

A

BLUE COLORED COMPOUND

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12
Q

RELATIONSHIP OF THE ABS TO AMOUNT OF NADH AND LD ACTIVITY IN THE WACKER COLORIMETRIC METHOD

A

DIECTLY PROPORTIONAL

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13
Q

[DIRECTION OF REACTION]
COLORIMETRIC METHOD BASED ON WACKER

A

LACTATE TO PYRUVATE

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14
Q

[DIRECTION OF REACTION]
WROBLEWSKI AND LA DUE

A

PYRUVATE TO LACTATE

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15
Q

PH OF THE REACTION IN THE WROBLEWSKI AND LA DUE METHOD

A

PH 7.4 TO 7.8

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16
Q

RELATIONSHIP OF NADH BOLUME ANDNLD ACTIVITY IN THE WROBLEWSKI AND LA DUE METHOD

A

INDIRECTLY PROPORTIONAL

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17
Q

DIFFERENCE IN THE SPEED OF REACTION IN THE FORWARD AND REVERSE REACTION IN THE WROBLEWSKI AND LA DUE METHOD

A

REVERSE IS THREE TIMES FASTER

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18
Q

[ADVANTAGES]
WROBLEWSKI AND LA DUE METHOD

A

SMALLER SAMPLE VOLUMES
SHORTER REACTION TIME

19
Q

[DISADVANTAGES]
WROBLEWSKI AND LA DUE METHOD

A

SUSCEPTIBLE TO SUBSTRATE EXHAUSTION
SUSCEPTIBLE TO LOSS OF LINEARITY

20
Q

METHODS FOR SEPARATION AND QUANTITATION OF LDH ISOENZYMES

A

ELECTROPHORESIS
COLUMN CHROMATOGRAPHY
IMMUNO-INHIBITION
BABSON’S METHOD
KAPLAN AND BERER
USE OF ALPHA HYDROXYBUTYRATE AS SUBSTRATE

21
Q

ELECTROPHORETIC MIGRATION OF THE LD ISOENZYMES

A

FASTEST — LDH1
SLOWEST — LDH6
BETWEEN LDH3/4 — LDH BOUND TO IMMUNOGLOBULIN (IgA/G)

22
Q

LDH ISOENZYME ISOLATED IN THE COLUMN CHROMATOGRAPHY METHOD

23
Q

METHOD USED FOR THE SCREENING OF AMI

A

COLUMN CHROMATOGRAPHY

24
Q

LDH ISOENZYME ISOLATED IN THE IMMUNO-INHIBITION METHOD

25
[PRINCIPLE] IMMUNO-INHIBITION METHOD
AB TO THE M SUB-UNIT OF LDH IS ADDED TO THE SERUM SAMPLE ANY ISOENZYME CONTAINING AN M-SUBUNIT WILL BE RENDERED INACTIVE ONLY LDH1 DOES NOT HAVE THE M-SUBUNIT
26
LDH SUBUNIT THAT DOES NOT CONTAIN THE M-SUBUNIT
LDH1
27
LDH ISOENZYMES MEASURED IN BABSON’S METHOD
LDH1 TO LDH5 RATIO
28
SOLUTION USED TO INHIBIT LDH ISOENZYMES IN BABSON’S METHOD
M-LACTATE — LDH1 M-LACTATE-1M UREA — LDH5
29
LDH ISOENZYME MEASURED IN THE KAPLAN AND BERGER METHOD
LDH1
30
[PRINCIPLE] KAPLAN AND BERGER
DETERMINATION OF LDH1 BY USING THE RATE OF HEAT PRODUCTION IN A BATCH TYPE OF COLORIMETER
31
LDH SUBUNIT WITH THE GREATER AFFINITY FOR ALPHA HYDROXYBUTYRATE
H SUBUNIT HAS A GREATER AFFINITY THAN THE M SUBUNIT
32
LD ISOENZYME MEASURED BY THE ALPHA HYDROXYBUTYRATE METHOD
LD1
33
[DISADVANTAGE] APLHA HYDROXYBUTYRATE METHOD
NOT SPECIFIC TO LD1 LD2,3,4 ALSO HAVE VARYING AMOUNTS OF THE H SUBUNIT
34
EFFECT OF HEMOLYZED SPECIMENS ON LDH ACTIVITY
FALSE INCREASE RBCs contain LDH activity 100-150x more than serum
35
DIFFERENCE OF SERUM AND RBC LDH ACTIVITY
RBCS HAVE LDH ACTIVITY 100-150X MORE THAN SERUM
36
EFFECT OF NOT ANALYZING THE SPECIMEN WITHIN 2 HOURS OF COLLECTION ON LDH ACTIVITY
FALSE DECREASE LDH is stable for 2-3 days at RT
37
WHEN DOES THE LOSS OF LDH ACTIVITY INTENSIFY IN SAMPLES
4C > 25C
38
STABILITY OF LDH ACTIVITY IN SERUM
2-3 DAYS AT RT
39
TIME LIMIT FOR THE ANALYZING OF LDH SAMPLES UPON COLLECTION
WITHIN 2 HOURS OF COLLECTION
40
COLD LABILE LDH ISOENZYME
LIVER LDH
41
EFFECT OF FROZEN AND THAWED SPECIMENS ON LDH ACTIVITY
FALSE DECREASE Liver LDH is cold labile and will be destroyed when frozen or thawed
42
EFFECT OF EDTA ON LDH ACTIVITY
FALSE DECREASE EDTA chelates zinc (activator)
43
LDH ACTIVATOR ION
ZINC
44
EFFECT OF PLASMA SPECIMENS ON LDH ACTIVITY
FALSE INCREASE Contamination with platelets which have LDH activity (LD3)

CLINICAL CHEMISTRY 2 (10 decks)

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