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CLINICAL CHEMISTRY 2 > [LAB] LDH > Flashcards

[LAB] LDH Flashcards

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1
Q

REFERENCE METHOD OF LDH

A

KINETIC METHOD BY BUHL AND JACKSON
MODIFIED BY WACKER

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2
Q

MAJOR ORGANS WHERE LDH IS DISTRIBUTED IN

A

HEART
LIVER
MUSCLE
KIDNEY

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3
Q

ELEVATED LEVELS OF SERUM LDH IS FOUND IN WHAT CLINICAL CONIDITONS

A

MI
LIVER DISEASE
RENAL DISEASE
ANEMIA
MALIGNANT DISEASES
PROGRESSIVE MUSCLE DYSTROPHY

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4
Q

IN WHAT DIRECTION CAN THE LD ENZYME BE MEASURED

A

FORWARD
REVERSE

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5
Q

[ADVANTAGES]
LACTATE TO PYRUVATE METHOD

A

BETTER REAGENT STABILITY
ELIMINATION OF PRE-INCUBATION
LINEAR RATE OF REACTION
LESS CONTAMINATION OF NAD

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6
Q

PRINCIPLE OF LDH

A

L-LACTATE + NAD
<—LDH (PH 8.3 TO 9.0) —>
PYRUVATE + NADH + H

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7
Q

COENZYME OF LDH

A

NAD

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8
Q

DOES LDH ACT ON D-LACTATE

A

NO

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9
Q

WHAT HAPPENS TO PYRUVATE AFTER FORMATION

A

PROCEED TO THE EMBDEN-MEYERHOFF PATHWAY
FOR THE GENERATION OF ENERGY

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10
Q

SOLUTIONS MIXED WITH NADH IN THE WACKER COLORIMETRIC METHOD

A

PHENAZINE METHOSULFATE
NITROBLUE TETRAZOLIUM

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11
Q

END PRODUCT OF THE WACKER COLORIMETRIC METHOD

A

BLUE COLORED COMPOUND

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12
Q

RELATIONSHIP OF THE ABS TO AMOUNT OF NADH AND LD ACTIVITY IN THE WACKER COLORIMETRIC METHOD

A

DIECTLY PROPORTIONAL

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13
Q

[DIRECTION OF REACTION]
COLORIMETRIC METHOD BASED ON WACKER

A

LACTATE TO PYRUVATE

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14
Q

[DIRECTION OF REACTION]
WROBLEWSKI AND LA DUE

A

PYRUVATE TO LACTATE

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15
Q

PH OF THE REACTION IN THE WROBLEWSKI AND LA DUE METHOD

A

PH 7.4 TO 7.8

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16
Q

RELATIONSHIP OF NADH BOLUME ANDNLD ACTIVITY IN THE WROBLEWSKI AND LA DUE METHOD

A

INDIRECTLY PROPORTIONAL

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17
Q

DIFFERENCE IN THE SPEED OF REACTION IN THE FORWARD AND REVERSE REACTION IN THE WROBLEWSKI AND LA DUE METHOD

A

REVERSE IS THREE TIMES FASTER

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18
Q

[ADVANTAGES]
WROBLEWSKI AND LA DUE METHOD

A

SMALLER SAMPLE VOLUMES
SHORTER REACTION TIME

19
Q

[DISADVANTAGES]
WROBLEWSKI AND LA DUE METHOD

A

SUSCEPTIBLE TO SUBSTRATE EXHAUSTION
SUSCEPTIBLE TO LOSS OF LINEARITY

20
Q

METHODS FOR SEPARATION AND QUANTITATION OF LDH ISOENZYMES

A

ELECTROPHORESIS
COLUMN CHROMATOGRAPHY
IMMUNO-INHIBITION
BABSON’S METHOD
KAPLAN AND BERER
USE OF ALPHA HYDROXYBUTYRATE AS SUBSTRATE

21
Q

ELECTROPHORETIC MIGRATION OF THE LD ISOENZYMES

A

FASTEST — LDH1
SLOWEST — LDH6
BETWEEN LDH3/4 — LDH BOUND TO IMMUNOGLOBULIN (IgA/G)

22
Q

LDH ISOENZYME ISOLATED IN THE COLUMN CHROMATOGRAPHY METHOD

A

LDH1
LDH2

23
Q

METHOD USED FOR THE SCREENING OF AMI

A

COLUMN CHROMATOGRAPHY

24
Q

LDH ISOENZYME ISOLATED IN THE IMMUNO-INHIBITION METHOD

A

LDH1

25
Q

[PRINCIPLE]
IMMUNO-INHIBITION METHOD

A

AB TO THE M SUB-UNIT OF LDH IS ADDED TO THE SERUM SAMPLE
ANY ISOENZYME CONTAINING AN M-SUBUNIT WILL BE RENDERED INACTIVE
ONLY LDH1 DOES NOT HAVE THE M-SUBUNIT

26
Q

LDH SUBUNIT THAT DOES NOT CONTAIN THE M-SUBUNIT

A

LDH1

27
Q

LDH ISOENZYMES MEASURED IN BABSON’S METHOD

A

LDH1 TO LDH5 RATIO

28
Q

SOLUTION USED TO INHIBIT LDH ISOENZYMES IN BABSON’S METHOD

A

M-LACTATE — LDH1
M-LACTATE-1M UREA — LDH5

29
Q

LDH ISOENZYME MEASURED IN THE KAPLAN AND BERGER METHOD

A

LDH1

30
Q

[PRINCIPLE]
KAPLAN AND BERGER

A

DETERMINATION OF LDH1 BY USING THE RATE OF HEAT PRODUCTION IN A BATCH TYPE OF COLORIMETER

31
Q

LDH SUBUNIT WITH THE GREATER AFFINITY FOR ALPHA HYDROXYBUTYRATE

A

H SUBUNIT HAS A GREATER AFFINITY THAN THE M SUBUNIT

32
Q

LD ISOENZYME MEASURED BY THE ALPHA HYDROXYBUTYRATE METHOD

A

LD1

33
Q

[DISADVANTAGE]
APLHA HYDROXYBUTYRATE METHOD

A

NOT SPECIFIC TO LD1
LD2,3,4 ALSO HAVE VARYING AMOUNTS OF THE H SUBUNIT

34
Q

EFFECT OF HEMOLYZED SPECIMENS ON LDH ACTIVITY

A

FALSE INCREASE
RBCs contain LDH activity 100-150x more than serum

35
Q

DIFFERENCE OF SERUM AND RBC LDH ACTIVITY

A

RBCS HAVE LDH ACTIVITY 100-150X MORE THAN SERUM

36
Q

EFFECT OF NOT ANALYZING THE SPECIMEN WITHIN 2 HOURS OF COLLECTION ON LDH ACTIVITY

A

FALSE DECREASE
LDH is stable for 2-3 days at RT

37
Q

WHEN DOES THE LOSS OF LDH ACTIVITY INTENSIFY IN SAMPLES

A

4C > 25C

38
Q

STABILITY OF LDH ACTIVITY IN SERUM

A

2-3 DAYS AT RT

39
Q

TIME LIMIT FOR THE ANALYZING OF LDH SAMPLES UPON COLLECTION

A

WITHIN 2 HOURS OF COLLECTION

40
Q

COLD LABILE LDH ISOENZYME

A

LIVER LDH

41
Q

EFFECT OF FROZEN AND THAWED SPECIMENS ON LDH ACTIVITY

A

FALSE DECREASE
Liver LDH is cold labile and will be destroyed when frozen or thawed

42
Q

EFFECT OF EDTA ON LDH ACTIVITY

A

FALSE DECREASE
EDTA chelates zinc (activator)

43
Q

LDH ACTIVATOR ION

A

ZINC

44
Q

EFFECT OF PLASMA SPECIMENS ON LDH ACTIVITY

A

FALSE INCREASE
Contamination with platelets which have LDH activity (LD3)

CLINICAL CHEMISTRY 2 (10 decks)

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