Lab Exam 1 Flashcards
What is the ocular lens?
Lenses you look through
What is the formula for image magnification ?
Objective power x ocular power
What is the objective lens?
The lens that is closest to the object
What is the revolving nose piece?
It holds the objectives
What is the coarse focus knob?
Raises and lowers the stage allowing you to focus the image
What is the fine focus knob?
Raises the stage by tiny amounts and brings the image into sharp focus
What is the stage?
Holds the glass microscope slide containing the specimen
What is the light source
Built into the microscope
What is the arm?
Let’s you carry the microscope with one hand, while the other holds the microscope underneath
What is the iris diaphragm?
Controls the amount of light entering the lens system
What is the substage condenser?
Focuses light on the subject
What objective is the nosepiece supposed to be on when you remove a slide?
4x
What is the objective power of the scanning objective?
4x
What is the objective power of a medium power objective?
10x
What is the objective power for a high power objective?
40x
What is the objective power of an oil immersion objective?
100x
What microscope allows for the largest image magnification?
Compound
What happens to the field of view as you change it from low to high magnification!
The higher magnification the smaller the field of view
What is the body tube
The body tube is the tube part of the oculars
What is the shape of cocci?
Small spheres
What is the bacilli shaped as?
Rod shaped
What is spirilla shaped like?
Corkscrew shaped
What color is gram positive bacteria?
Violet
What color is gram negative bacteria?
Pink/red
What is the formula for Magnification of a drawing?
Drawing size (um)
________________
Measured cell size (um)
How do you convert from mm to um?
Multiply by 1000.
Ex: 2.7mm x 1000 = 2700um
How do you measure the length of something?
Put it on leftmost side and see how much space is takes up and multiply it by how big the field diameter is.
How do you calculate field diameter?
4(x) x 5mm = 10(next objective) x fd10x
Fd10x = 4 x 5mm /10x = 2mm
How do you calculate grams of solute?
Molecular weight (grams/mole) x volume (litre) x molarity (moles/litre)
What is the formula for preparing dilutions?
M1v1 = m2v2
Final m solution x final liter
Divided by stock solution
