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Cell Biology > Lab Exam 1 > Flashcards

Lab Exam 1 Flashcards

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0
Q

What is the ocular lens?

A

Lenses you look through

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1
Q

What is the formula for image magnification ?

A

Objective power x ocular power

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2
Q

What is the objective lens?

A

The lens that is closest to the object

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3
Q

What is the revolving nose piece?

A

It holds the objectives

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4
Q

What is the coarse focus knob?

A

Raises and lowers the stage allowing you to focus the image

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5
Q

What is the fine focus knob?

A

Raises the stage by tiny amounts and brings the image into sharp focus

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6
Q

What is the stage?

A

Holds the glass microscope slide containing the specimen

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7
Q

What is the light source

A

Built into the microscope

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8
Q

What is the arm?

A

Let’s you carry the microscope with one hand, while the other holds the microscope underneath

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9
Q

What is the iris diaphragm?

A

Controls the amount of light entering the lens system

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10
Q

What is the substage condenser?

A

Focuses light on the subject

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11
Q

What objective is the nosepiece supposed to be on when you remove a slide?

A

4x

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12
Q

What is the objective power of the scanning objective?

A

4x

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13
Q

What is the objective power of a medium power objective?

A

10x

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14
Q

What is the objective power for a high power objective?

A

40x

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15
Q

What is the objective power of an oil immersion objective?

A

100x

16
Q

What microscope allows for the largest image magnification?

A

Compound

17
Q

What happens to the field of view as you change it from low to high magnification!

A

The higher magnification the smaller the field of view

18
Q

What is the body tube

A

The body tube is the tube part of the oculars

19
Q

What is the shape of cocci?

A

Small spheres

20
Q

What is the bacilli shaped as?

A

Rod shaped

21
Q

What is spirilla shaped like?

A

Corkscrew shaped

22
Q

What color is gram positive bacteria?

A

Violet

23
Q

What color is gram negative bacteria?

A

Pink/red

24
Q

What is the formula for Magnification of a drawing?

A

Drawing size (um)
________________
Measured cell size (um)

25
Q

How do you convert from mm to um?

A

Multiply by 1000.

Ex: 2.7mm x 1000 = 2700um

26
Q

How do you measure the length of something?

A

Put it on leftmost side and see how much space is takes up and multiply it by how big the field diameter is.

27
Q

How do you calculate field diameter?

A

4(x) x 5mm = 10(next objective) x fd10x

Fd10x = 4 x 5mm /10x = 2mm

28
Q

How do you calculate grams of solute?

A

Molecular weight (grams/mole) x volume (litre) x molarity (moles/litre)

29
Q

What is the formula for preparing dilutions?

A

M1v1 = m2v2
Final m solution x final liter
Divided by stock solution

Cell Biology (13 decks)

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