Lab content Flashcards
Wuchereria bancrofti - disease
Lymphatic filiariasis - elephantiasis, lymphoedema caused by adult worms in the lymphatic vessels
Wuchereria bancrofti - vector
Culcine and anopheles mosquitoes
Wuchereria bancrofti - giemsa staining features
- Pink sheath
- No nucleus in the tail (BANned)
Brugia malayi - disease
Lymphatic filariasis due to adult worms in the lymphatics
Brugia malayi - vectors
Mansonia, Anopheles mosquitoes
Brugia malayi - giemsa staining features
- Pink sheath
- 2 nuclei in the tail (two for bru)
Loa loa - disease
Eye migration
Calabar swellings (hypersensivitiy reactions)
Loa loa - vector
Deerfly (chrysops)
Loa loa - features on giemsa staining
- Ghost sheath (does not stain)
- Flattened nuclei go all the way to the end (lower and lower)
Onchocerca volvulus - giemsa staining
- No sheath
- No nucleus in the tail
- Bigger than mansonella
Mansonella species - giemsa staining
Smaller and narrower
Small nuclei at the end (shepherd’s hook)
Mansonella vector
Midges/blackflies
Sandfly features
Sandy coloured, very hairy body and wings
Blackfly features
Hunchback, very clear wings
Biting midge features
Wings with dark and paler patchiness
Anopheles vs culcine heads
- Palps. Short —> Female culcine. If long…
- If the antennae are fluffy it’s a male
- Then to distinguish if it’s anopheles or culcine –> has a more spade like antennae in anopheles
Tse tse fly
Meat cleaver feature in the wing
Deerfly - key feature and disease
Hexagon feature in the wing with dark bands features
Loa loa
Culex mosquito key feature
Boring and brown
Haemagogus mosquito key feature
Bright metallic sheen
Mansonia mosquito key feature
Salt and pepper leaves on wings
Aedes mosquito key feature
Stripey legs, white markings like a violin on its back
P falciparum - ring stage key features
Small delicate rings with chromatin dots
Few spots (Maurer’s clefts)
Accoles on the outside of the cell
RBC NOT enlarged
P vivax - ring stage key features
Not such a neat ring form
Lots more dots (although not too dark) - schuffner’s
RBC enlarged
P ovale ring stage
Compact but thick rings
Darkers dots
May have fimbriation
Tend not to be as enlarged as vivax
P malariae - ring stage key features
Small compact rings
No dots
Triatomine head has antennae inserted at the end of the head
Rhodnius
Triatomine head has antennae inserted mid way along the head
Triatoma
Triatomine head has antennae inserted just in front of the eyes and has a short chubby head
Panstrongylus
Mosquito egg has little floats on the side
Anopheles
Mosquito eggs in a collection like a raft
Culex (think cullection)
Mosquito eggs are in a group stuck to some vegetation
Mansonia
Mosquito eggs are boring cigar shaped with no floads
Aedes
Mosquito larva which has no siphon
Anopheles
Mosquito larva which has a siphon
Culex/aedes
Mosquito larva which has a cerrated point for a siphon
Mandonia (think it likes to stick to vegetation)
Larva - looks like a white space hopper
Tse tse fly larva
Larva - looks like a black space hopper
Tse tse fly pupa
Upright pupa
Sandfly
Pupa sits in a slipper shaped cocoon
Blackfly
How to distinguish between clonorchis and opisthorcis adults
Opisthorcis have lobulated testes, rather than branched
Treatment for Trypanosoma Brucei
Pentamidine
Deep cavity and pointed tail in filariform L3 larvae
Hookworm
Deep buccal cavity at the head of this rhabditiform L1 larvae
Hookworm
Periorbital odoema and GI symptoms after eating undercooked sausages
Trichinella
Onchocerciasis vector
Black fly (river blindness — see black)
Wuchereria Bancrofti mosquito
Culex aedes anopheles
Brugia malayi mosquito
Mansoni