Lab content

Question 1

Wuchereria bancrofti - disease

Answer 1

Lymphatic filiariasis - elephantiasis, lymphoedema caused by adult worms in the lymphatic vessels

Question 2

Wuchereria bancrofti - vector

Answer 2

Culcine and anopheles mosquitoes

Question 3

Wuchereria bancrofti - giemsa staining features

Answer 3

1. Pink sheath
2. No nucleus in the tail (BANned)

Question 4

Brugia malayi - disease

Answer 4

Lymphatic filariasis due to adult worms in the lymphatics

Question 5

Brugia malayi - vectors

Answer 5

Mansonia, Anopheles mosquitoes

Question 6

Brugia malayi - giemsa staining features

Answer 6

1. Pink sheath
2. 2 nuclei in the tail (two for bru)

Question 7

Loa loa - disease

Answer 7

Eye migration
Calabar swellings (hypersensivitiy reactions)

Question 8

Loa loa - vector

Answer 8

Deerfly (chrysops)

Question 9

Loa loa - features on giemsa staining

Answer 9

1. Ghost sheath (does not stain)
2. Flattened nuclei go all the way to the end (lower and lower)

Question 10

Onchocerca volvulus - giemsa staining

Answer 10

1. No sheath
2. No nucleus in the tail
3. Bigger than mansonella

Question 11

Mansonella species - giemsa staining

Answer 11

Smaller and narrower
Small nuclei at the end (shepherd’s hook)

Question 12

Mansonella vector

Answer 12

Midges/blackflies

Question 13

Sandfly features

Answer 13

Sandy coloured, very hairy body and wings

Question 14

Blackfly features

Answer 14

Hunchback, very clear wings

Question 15

Biting midge features

Answer 15

Wings with dark and paler patchiness

Question 16

Anopheles vs culcine heads

Answer 16

1. Palps. Short —> Female culcine. If long…
2. If the antennae are fluffy it’s a male
3. Then to distinguish if it’s anopheles or culcine –> has a more spade like antennae in anopheles

Question 17

Tse tse fly

Answer 17

Meat cleaver feature in the wing

Question 18

Deerfly - key feature and disease

Answer 18

Hexagon feature in the wing with dark bands features

Loa loa

Question 19

Culex mosquito key feature

Answer 19

Boring and brown

Question 20

Haemagogus mosquito key feature

Answer 20

Bright metallic sheen

Question 21

Mansonia mosquito key feature

Answer 21

Salt and pepper leaves on wings

Question 22

Aedes mosquito key feature

Answer 22

Stripey legs, white markings like a violin on its back

Question 23

P falciparum - ring stage key features

Answer 23

Small delicate rings with chromatin dots
Few spots (Maurer’s clefts)
Accoles on the outside of the cell
RBC NOT enlarged

Question 24

P vivax - ring stage key features

Answer 24

Not such a neat ring form
Lots more dots (although not too dark) - schuffner’s
RBC enlarged

Question 25

P ovale ring stage

Answer 25

Compact but thick rings Darkers dots May have fimbriation Tend not to be as enlarged as vivax

Question 26

P malariae - ring stage key features

Answer 26

Small compact rings No dots

Question 27

Triatomine head has antennae inserted at the end of the head

Answer 27

Rhodnius

Question 28

Triatomine head has antennae inserted mid way along the head

Answer 28

Triatoma

Question 29

Triatomine head has antennae inserted just in front of the eyes and has a short chubby head

Answer 29

Panstrongylus

Question 30

Mosquito egg has little floats on the side

Answer 30

Anopheles

Question 31

Mosquito eggs in a collection like a raft

Answer 31

Culex (think cullection)

Question 32

Mosquito eggs are in a group stuck to some vegetation

Answer 32

Mansonia

Question 33

Mosquito eggs are boring cigar shaped with no floads

Answer 33

Aedes

Question 34

Mosquito larva which has no siphon

Answer 34

Anopheles

Question 35

Mosquito larva which has a siphon

Answer 35

Culex/aedes

Question 36

Mosquito larva which has a cerrated point for a siphon

Answer 36

Mandonia (think it likes to stick to vegetation)

Question 37

Larva - looks like a white space hopper

Answer 37

Tse tse fly larva

Question 38

Larva - looks like a black space hopper

Answer 38

Tse tse fly pupa

Question 39

Upright pupa

Answer 39

Sandfly

Question 40

Pupa sits in a slipper shaped cocoon

Answer 40

Blackfly

Question 41

How to distinguish between clonorchis and opisthorcis adults

Answer 41

Opisthorcis have lobulated testes, rather than branched

Question 42

Treatment for Trypanosoma Brucei

Answer 42

Pentamidine

Question 43

Deep cavity and pointed tail in filariform L3 larvae

Answer 43

Hookworm

Question 44

Deep buccal cavity at the head of this rhabditiform L1 larvae

Answer 44

Hookworm

Question 45

Periorbital odoema and GI symptoms after eating undercooked sausages

Answer 45

Trichinella

Question 46

Onchocerciasis vector

Answer 46

Black fly (river blindness — see black)

Question 47

Wuchereria Bancrofti mosquito

Answer 47

Culex aedes anopheles

Question 48

Brugia malayi mosquito

Answer 48

Mansoni