(Lab 3) Cumulative & Organisms to Know Flashcards by Rusty Shackleford

What stain was used? What is the appearance of the result? Interpret the result.

Gram stain
Pink
Gram negative

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What stain was used? What is the appearance of the result? Interpret the result.

Gram stain
Purple
Gram Positive

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What test is being performed? Interpret the result indicated by the arrow.

Catalase test
positive

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What test is being performed? Interpret the result indicated by the arrow.

Catalase test
negative

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Use organism B for the following questions.
What stain was used? What is the appearance of the result? Interpret the result.

Acid-fast stain
Magenta
Acid-fast

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Use organism A for the following questions.
What stain was used? What is the appearance of the result? Interpret the result.

Acid-fast
Blue
Non-acid-fast

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What stain was used? What is the appearance of the result? Interpret the result.

Endospore stain
Green Spores
Positive

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What test is being performed? Interpret the result

Oxidase test
Positive

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What test is being performed? Interpret the result

Oxidase test
Negative

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What test is being performed? Interpret the result

Fluid thioglycolate broth

Facultative anaerobe

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What test is being performed? Interpret the result

Fluid thioglycolate broth

Obligate anaerobe

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What test is being performed? Interpret the result

Fluid thioglycolate broth

Microaerophile

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What test is being performed? Interpret the result

Fluid thioglycolate broth

Obligate Aerobe

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What test is being performed? Interpret the result

Carbohydrate Fermentation Test

Yellow: positive (+A)
- sugar fermentation without gas

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What test is being performed? Interpret the result

Carbohydrate Fermentation Test

Yellow: positive (+AG)
- sugar fermentation with gas

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What test is being performed? Interpret the result

Carbohydrate Fermentation Test

Orange/red: negative (-)
- no sugar fermentation

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What test is being performed? Interpret the result

Carbohydrate Fermentation Test

pink: negative (-alk)
- no sugar fermentation
- protein digestion

In a nitrate reduction test, you add Nitrate reagents A & B. The media turns red. What are your results?

Nitrite (NO2-) is present
Bacteria can use Nitrate (NO3-) via Nitrate reductase

In a nitrate reduction test, you add Nitrate reagents A & B + Zn. The media turns red. What are your results?

Nitrate (NO3-) was present. Reduced by Zinc.
Bacteria cannot use Nitrate (NO3-). Negative for Nitrate/Nitrite reductase

In a nitrate reduction test, you add Nitrate reagents A & B + Zn. The media turns yellow. What are your results?

Nitrogen gas (N2) or Ammonia (NH3) is present.
Bacteria can use Nitrate (NO3-) via Nitrate reductase and further reduce it via Nitrite reductase

ID the organism. What Classification does it belong to? What are its notable features?

Saccharomyces cerevisiae

Fungi: Yeast

Performs budding

ID the organism. What Classification does it belong to? What are its notable features?

Amoeba proteus

Free-living protozoan: Sarcodina (Pseudopods)

Cytoplasmic streaming

ID the organism. What Classification does it belong to? What are its notable features?

Entamoeba histolytica (trophozoite)

Parasitic protozoan: Sarcodina (Pseudopods)

Amoebic dysentery
RBCs in the cell
Oral-fecal transmission
cyst form is infectious stage
Trophozoite is parasitic stage. Lives in intestines

What are the possible results of an MSA plate?

No growth: non-halotolerant

Growth: halotolerant

yellow growth: ferment mannitol

pink growth: use of proteins instead of mannitol

ID the organism. What Classification does it belong to? What are its notable features?

Necator americanus (adult male) Nematoda - Hookworm -> anemia - Fomite transmisssion via soil - larvae form infectious. Looks similar to pinworm, but it's smaller under the microscope with no eggs

What is the etiology of Syphilis? Describe the organism and signs/symptoms

Treponema pallidum G- Spirochete s/s: - chancre at infection site - rash - Permanent damage ("gumma", neuro, joints)

ID the organism. What Classification does it belong to? What are its notable features?

Rhizopus stolonifer Mold - sporangium - nonseptate hyphae

ID the organism. What Classification does it belong to? What are its notable features?

Aspergillus niger Mold - septate hyphae - conidia

ID the organism. What Classification does it belong to? What are its notable features?

Penicillium notatum Mold -septate hyphae - conidia

ID the organism. What Classification does it belong to? What are its notable features?

Stentor Ciliates (cilia) - has cytosome

ID the organism. What Classification does it belong to? What are its notable features?

Paramecium caudatum Ciliates (cilia) - has contractile vacuoles and cytosome

ID the organism. What Classification does it belong to? What are its notable features?

Euglena Mastigophora/flagellate (flagella) "eye spots"

ID the organism. What Classification does it belong to? What are its notable features?

Volvox Mastigophora/flagellate (flagella) Gonidia for reproduction

ID the organism. What Classification does it belong to? What are its notable features?

Balantidium coli Ciliophora (cilia) Disease: Balantidiasis (rare form of dysentery) Oral-fecal transmission - cyst form can survive in water Trophozite lives in intestine Only ciliated human pathogen

ID the organism. What Classification does it belong to? What are its notable features?

Trichomonas vaginalis Mastigophora (flagella) Disease: Trichomoniasis (STD) - male: asymptomatic - female: itching and discharge Trophozite form found in vagina or penis. No egg form

ID the organism. What Classification does it belong to? What are its notable features?

Trypanosoma brucei (trypomastigote) Mastigophora (flagella) Disease: African Sleeping sickness Vector transmission: Tsetse fly Kinetoplast: specialized region of mitochondria with kDNA

ID the organism. What Classification does it belong to? What are its notable features?

Plasmodium vivax, falciparum, and malariae Apicomplexa (gliding motion) Disease: Malaria - falciparum:acute and fatal - vivax: mild, chronic, recurring Vector: female Anopheles mosquito Life cycle: Mosquito saliva infective stage delivers sporozite->enters liver, asexual reproduction-> released as merozites -> enters RBC -> ring stage-> asexual reproduction becomes schizont -> RBC ruptures and release gametes

ID the organism. What Classification does it belong to? What are its notable features?

Schistosoma mansoni (with cercaria larvae) trematode Disease: Schistosomiasis Waterborne Transmission Males have gynecophoric groove Lifecycle: eggs released in feces and urine-> hatch into miracidia-> infect snails (intermediate host) -> release cercaria larvae and burrow into human skin -> adults in Hepatic portal vein and lay eggs -> egg movement to gut and urine bladder

ID the organism. What Classification does it belong to? What are its notable features?

Enterobius vermicularis nematode Disease: pinworm Sx: itchy rectum Larger with eggs, transmitted by ingesting eggs

ID the organism. What Classification does it belong to? What are its notable features?

Taenia (Proglottids, Gravid Proglottids, and Scolex) cestoda Disease: tapeworm Transmission: - saginata: undercooked beef - solium: undercooked pork

ID the organism. What Classification does it belong to? What are its notable features?

Trichinella spiralis nematode Disease: Trichinosis Transmission: undercooked contaminated pork Larvae encyst in striated muscle