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MICR 262 > (Lab 3) Cumulative & Organisms to Know > Flashcards

(Lab 3) Cumulative & Organisms to Know Flashcards

1
Q

What stain was used? What is the appearance of the result? Interpret the result.

A

Gram stain
Pink
Gram negative

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2
Q

What stain was used? What is the appearance of the result? Interpret the result.

A

Gram stain
Purple
Gram Positive

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3
Q

What test is being performed? Interpret the result indicated by the arrow.

A

Catalase test
positive

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4
Q

What test is being performed? Interpret the result indicated by the arrow.

A

Catalase test
negative

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5
Q

Use organism B for the following questions.
What stain was used? What is the appearance of the result? Interpret the result.

A

Acid-fast stain
Magenta
Acid-fast

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6
Q

Use organism A for the following questions.
What stain was used? What is the appearance of the result? Interpret the result.

A

Acid-fast
Blue
Non-acid-fast

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7
Q

What stain was used? What is the appearance of the result? Interpret the result.

A

Endospore stain
Green Spores
Positive

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8
Q

What test is being performed? Interpret the result

A

Oxidase test
Positive

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9
Q

What test is being performed? Interpret the result

A

Oxidase test
Negative

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10
Q

What test is being performed? Interpret the result

A

Fluid thioglycolate broth

Facultative anaerobe

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11
Q

What test is being performed? Interpret the result

A

Fluid thioglycolate broth

Obligate anaerobe

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12
Q

What test is being performed? Interpret the result

A

Fluid thioglycolate broth

Microaerophile

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13
Q

What test is being performed? Interpret the result

A

Fluid thioglycolate broth

Obligate Aerobe

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14
Q

What test is being performed? Interpret the result

A

Carbohydrate Fermentation Test

Yellow: positive (+A)
- sugar fermentation without gas

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15
Q

What test is being performed? Interpret the result

A

Carbohydrate Fermentation Test

Yellow: positive (+AG)
- sugar fermentation with gas

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16
Q

What test is being performed? Interpret the result

A

Carbohydrate Fermentation Test

Orange/red: negative (-)
- no sugar fermentation

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17
Q

What test is being performed? Interpret the result

A

Carbohydrate Fermentation Test

pink: negative (-alk)
- no sugar fermentation
- protein digestion

18
Q

In a nitrate reduction test, you add Nitrate reagents A & B. The media turns red. What are your results?

A

Nitrite (NO2-) is present
Bacteria can use Nitrate (NO3-) via Nitrate reductase

19
Q

In a nitrate reduction test, you add Nitrate reagents A & B + Zn. The media turns red. What are your results?

A

Nitrate (NO3-) was present. Reduced by Zinc.
Bacteria cannot use Nitrate (NO3-). Negative for Nitrate/Nitrite reductase

20
Q

In a nitrate reduction test, you add Nitrate reagents A & B + Zn. The media turns yellow. What are your results?

A

Nitrogen gas (N2) or Ammonia (NH3) is present.
Bacteria can use Nitrate (NO3-) via Nitrate reductase and further reduce it via Nitrite reductase

21
Q

ID the organism. What Classification does it belong to? What are its notable features?

A

Saccharomyces cerevisiae

Fungi: Yeast

Performs budding

22
Q

ID the organism. What Classification does it belong to? What are its notable features?

A

Amoeba proteus

Free-living protozoan: Sarcodina (Pseudopods)

Cytoplasmic streaming

23
Q

ID the organism. What Classification does it belong to? What are its notable features?

A

Entamoeba histolytica (trophozoite)

Parasitic protozoan: Sarcodina (Pseudopods)

  • Amoebic dysentery
  • RBCs in the cell
  • Oral-fecal transmission
  • cyst form is infectious stage
  • Trophozoite is parasitic stage. Lives in intestines
24
Q

What are the possible results of an MSA plate?

A

No growth: non-halotolerant

Growth: halotolerant

yellow growth: ferment mannitol

pink growth: use of proteins instead of mannitol

25
Q

ID the organism. What Classification does it belong to? What are its notable features?

A

Necator americanus (adult male)

Nematoda

  • Hookworm -> anemia
  • Fomite transmisssion via soil
  • larvae form infectious. Looks similar to pinworm, but it’s smaller under the microscope with no eggs
26
Q

What is the etiology of Syphilis? Describe the organism and signs/symptoms

A

Treponema pallidum

G- Spirochete

s/s: - chancre at infection site
- rash
- Permanent damage (“gumma”, neuro, joints)

27
Q

ID the organism. What Classification does it belong to? What are its notable features?

A

Rhizopus stolonifer

Mold

  • sporangium
  • nonseptate hyphae
28
Q

ID the organism. What Classification does it belong to? What are its notable features?

A

Aspergillus niger

Mold

  • septate hyphae
  • conidia
29
Q

ID the organism. What Classification does it belong to? What are its notable features?

A

Penicillium notatum

Mold

-septate hyphae
- conidia

30
Q

ID the organism. What Classification does it belong to? What are its notable features?

A

Stentor

Ciliates (cilia)

  • has cytosome
31
Q

ID the organism. What Classification does it belong to? What are its notable features?

A

Paramecium caudatum

Ciliates (cilia)

  • has contractile vacuoles and cytosome
32
Q

ID the organism. What Classification does it belong to? What are its notable features?

A

Euglena

Mastigophora/flagellate (flagella)

“eye spots”

33
Q

ID the organism. What Classification does it belong to? What are its notable features?

A

Volvox

Mastigophora/flagellate (flagella)

Gonidia for reproduction

34
Q

ID the organism. What Classification does it belong to? What are its notable features?

A

Balantidium coli

Ciliophora (cilia)

Disease: Balantidiasis (rare form of dysentery)

Oral-fecal transmission
- cyst form can survive in water

Trophozite lives in intestine

Only ciliated human pathogen

35
Q

ID the organism. What Classification does it belong to? What are its notable features?

A

Trichomonas vaginalis

Mastigophora (flagella)

Disease: Trichomoniasis (STD)
- male: asymptomatic
- female: itching and discharge

Trophozite form found in vagina or penis. No egg form

36
Q

ID the organism. What Classification does it belong to? What are its notable features?

A

Trypanosoma brucei (trypomastigote)

Mastigophora (flagella)

Disease: African Sleeping sickness

Vector transmission: Tsetse fly

Kinetoplast: specialized region of mitochondria with kDNA

37
Q

ID the organism. What Classification does it belong to? What are its notable features?

A

Plasmodium vivax, falciparum, and malariae

Apicomplexa (gliding motion)

Disease: Malaria
- falciparum:acute and fatal
- vivax: mild, chronic, recurring
Vector: female Anopheles mosquito

Life cycle: Mosquito saliva infective stage delivers sporozite->enters liver, asexual reproduction-> released as merozites -> enters RBC -> ring stage-> asexual reproduction becomes schizont -> RBC ruptures and release gametes

38
Q

ID the organism. What Classification does it belong to? What are its notable features?

A

Schistosoma mansoni (with cercaria larvae)

trematode

Disease: Schistosomiasis

Waterborne Transmission

Males have gynecophoric groove

Lifecycle: eggs released in feces and urine-> hatch into miracidia-> infect snails (intermediate host) -> release cercaria larvae and burrow into human skin -> adults in Hepatic portal vein and lay eggs -> egg movement to gut and urine bladder

39
Q

ID the organism. What Classification does it belong to? What are its notable features?

A

Enterobius vermicularis

nematode

Disease: pinworm

Sx: itchy rectum

Larger with eggs, transmitted by ingesting eggs

40
Q

ID the organism. What Classification does it belong to? What are its notable features?

A

Taenia (Proglottids, Gravid Proglottids, and Scolex)

cestoda

Disease: tapeworm

Transmission:
- saginata: undercooked beef
- solium: undercooked pork

41
Q

ID the organism. What Classification does it belong to? What are its notable features?

A

Trichinella spiralis

nematode

Disease: Trichinosis

Transmission: undercooked contaminated pork

Larvae encyst in striated muscle

MICR 262 (30 decks)

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