Lab 13 Flashcards
refers to the information about the metabolism of bacteria
Biologic Characters
the chemical reactions that occur within all living organisms
metabolism
proteins that catalyze biological reactions
enzymes
the majority of enzyme which function inside a cell
endoenzymes
many bacteria make these enzymes, which are released from the cell to catalyze reactions outside the cell
exoenzymes
chemical reactions that release energy from the decomposition of complex organic molecules
catabolism
organic molecules that contain carbon, hydrogen, and oxygen in the ration (CH2O)n
carbohydrates
simple sugars containing 3-7 carbons
monosaccharides
composed of 2-20 monosaccharide molecules
oligosaccharides
the most common oligosaccharide
disaccharides
consist of 20 or more monosaccharide molecules
polysaccharides
Exoenzymes are mainly _________ that leave the cell and break down, by the addition of water, large substrates into smaller components, which can then be transported into the cell.
hydrolytic enzymes
hydrolyzes the polysaccharide starch into smaller carbohydrates
amylase
a monosaccharide that can be released by hydrolysis
glucose
chemical reaction that requires the presence of molecular oxygen
oxidative catabolism
The presence of an _____ is determined by looking for a change in the substrate outside a bacterial colony.
exoenzyme
chemical reaction that does not require oxygen but may occur in its presence
fermentative catabolism
nutrient semisolid agar deep containing a high concentration of carbohydrate and a low concentration of peptone
OF medium
In OF medium, the ____ will support the growth of bacteria that don’t use the carbohydrate.
peptone
OF medium contains the indicator ______, which turns _____ in the presence of acids, indicating _____ of the carbohydrate.
OF medium contains the indicator bromthymol blue, which turns yellow in the presence of acids, indicating catabolism of the carbohydrate.
________, caused by he use of the peptone and not of carbohydrate, are indicated by a dark blue color due to ________ production.
Alkaline conditions, caused by the used of peptone and not of carbohydrate, are indicated by a dark blue color due to ammonia production.
If the carbohydrate is metabolized in both tubes, _____ has occurred.
If the carbohydrate is metabolized in both tubes, fermentation has occurred.
