Lab 12 Flashcards
Non-nucleated biconcave discs
Approximately 7-8um in diameter
Contain the oxygen carrying pigment known as hemoglobin
Red blood cells (Eryhrocytes)
Occur in a wide variety of forms
White blood cells (Leukocytes)
Two major groups of leukocytes are:
Granulocytes
Agranulocytes
Characterized by a large number of visible cytoplasmic granules
Granulocytes
Have no apparent cytoplasmic granules
Agranulocytes
Types of Granulocytes:
Neutophil
Eosinophil
Basophil
Types of Agranulocytes:
Lymphocyte
Monocyte
Multilobed nucleus
Inconspicuous, pale purple granules
60-70% of total Leukocytes
Neutophil
Bilobed nucleus
Many, large red granules
2-4% of total Leukocytes
Eosinophil
S-shaped nucleus
Many, large purple granules
0.5-1% of total Leukocytes
Basophil
Large round nucleus
No visible granules, thin rim of blue cytoplasm
20-25% of total Leukocytes
Lymphocyte
Large, horse shoe shaped nucleus
No visible granules, abundant pale blue cytoplasm
3-8% of total Leukocytes
Monocyte
The most numerous cells
Function in transport of oxygen and carbon dioxide
Stain pink for eosin stain
Red blood cells
Are white blood cells that contain visible granules of different sizes and colors
Granulocytes
Phagocytic, functioning in the destruction of pathogenic micro-organisms and other foreign matter
Range in size from 10-12 um in diamter
Segmented nucleus
Minute pink to violet granules
Only weakly reacts with either acidic eosin or basic methylene blue dyes of Wright’s stain
Neutrophils
Range from 10-12 um in diameter
Play a role in the control of local responses associated with allergic reactions
Important defense against multicellular parasites
Reddish-orange globular granules
Eosinophils
The most difficult granulocytes to locate
May be involved in allergic reactions, releasing herparin and histamine that enhance immune response
8-10 um
Large, dark purple granules
Affinity for methylene dye
Basophil
White blood cells that lack visible cytoplasmic granules
Agranulocytes
Most numerous of the agranular leuocytes
20-25%
Produce antibodies and other agents involved in the immune process
Can occur in 3 sizes
Lymphocytes
Largest of white blood cells 12-20 um Highly phagocytic Engulf large particles Light staining, blue violet nucleus
Monocytes
Blood also contains cell fragments called _______.
150,000 to 400,4000 per cubic milimeter of blood
Disc-shaped
2-4 um
No nucleus
Stop blood loss from damaged vessels by forming a plug
Platelets
The percentage of total blood volume occupied by RBCs
Hematocrit
Uses a series of lithographed colors that illustrate the tints of blood ranging from 10% to 100% hemoglobin
Tallquist Scale
An instrument which compares a hemolyzed sample of blood with a color of standard by moving a slide on the side back and forth until the two halves of the green field match
Grams Hb/100 ml
Hemoglobinometer
The blood groups are based on the presence or absence of genetically determined glycoproteins and glycolipids on the cell membrane of RBCs
Antigens
Found in blood plasma
Antibodies
Agglutinate means
Clump
Presence of antigen D in the blood
Rh positive
Do not have antigen D present in their blood
Rh negative
When mismatched blood is given to a recipient a ________ _______ may occur in which the recipients plasma antibodies recognize the antigens of the donor’s RBC as foreign and mount an immune response
Transfusion reaction
Transfusion rule
The recipient’s antibodies MUST NOT MATCH the donor’s antigens
A decreased amount of hemoglobin in the blood and consequently a decreased amount of oxygen reaches the tissue
Could result from decreased production of erythrocytes
Iron deficiency or b12
Anemia
Malformation of RBC
Sickle cell anemia
The ________ and ________ evaluations are the most important tests in the diagnosis of anemia
Hematocrit; hemoglobin
A condition characterized by above normal RBC counts
May be due to living in high altitude or red marrow malignancy
Polycythemia
Visual method to count RBC and WBC
Instrument consists of special microscope slide that contains two counting chambers
Hemacytometer
A white blood cell count of more than 10,000
Leukocytosis
White blood cell count significantly less than 5,000
Leukopenia
Blood normally clots within _ to _ minutes
2; 6
Conduct blood away from the heart
Arteries
Conduct blood towards the heart
Veins
Interconnecting the two major blood vessel types are microscopic:
Capillaries
Only consist of a single layer of endothelial cells (simple squamous cells) and a basement membrane
Capillaries
The lumen of the ____ is larger than that of the _____ , the actual wall however, is thinner
Vein; Artery
The shape of an _______ in cross-section is usually, but not always round. ______ tend to be more irregular and may be collapsed on the side
Artery; veins
The innermost layer composed of a single layer of endothelial cells. In some of the large arteries this layer also includes an internal elastic membrane (known as the elastica interna) which appears as thing, bright, scalloped line
Tunica interna (Intima) - Artery
The middle layer, consisting mainly of smooth muscle and elastic fibers running in a circular direction around the lumen
Tunica media - Artery
The outer layer, composed of fibrous connective tissue and fat cells. In some of the larger arteries the tunica media and tunica externa are separated by an external elastic membrane the elastica externa
Tunica externa (adventitia) - Artery
Consists of a layer of endothelial cells. There is no elastic interna.
Tunica interna - Vein
Composed mainly of circular smooth muscle cells
Tunica media - Vein
Composed of loose fibrous connective tissue with fat cells
Tunica externa - Vein