Lab

Question 1

Examples of gram positive cocci in chains

Answer 1

Group A B C Streptococcus

Enterococcus

Question 2

Gram positive cocci in pairs examples

Answer 2

Streptococcus pneumonia

Enterococcus (pairs or chain)

Question 3

Gram positive in clusters examples

Answer 3

Staphylococcus- MRSA, MSSA

Corynebacteria

Question 4

Gram positive rods

Answer 4

Clostridium
Bacillus
Listeria

Question 5

Gram negative cocci examples

Answer 5

Neisseria (pairs diplodocus)

Question 6

Gram negative rods example

Answer 6

Enterobacteriase (ecoli, klebsella)

Pseudomonas

Question 7

What sample can stay at room tem

Answer 7

Csf

Question 8

Teichoic acid and thick peptitoglycan layers are associated with

Answer 8

Gram positive bacteria

Question 9

What fluorescent stain is added that binds to chitin in cell wall of fungus

Answer 9

Calcofluor white

Question 10

What are the 3 ways bacteria transfer resistance

Answer 10

Transformation: dna from environment enter bacteria
Conjugation: direct contact between bacteria and plasmid exchange
Transduction: transferred to recipient cell from virus capable of infecting bacteria (bacteriophage)

Question 11

What kind of growth media supports the growth of a wide variety of bacteria?

Answer 11

Nutrient agar. Examples include trypticase soy agar with sheep blood

Question 12

Enrichment media

Answer 12

Contains special nutrients for the growth of fastidious(hard to grow) bacteria. Chocolate agar- growth of n.meningitis

Question 13

Selective media

Answer 13

Contains chemical or antibiotics designed to inhibit growth of normal commensal bacteria. Bismuth sulfate agar, macconkey’s agar

Question 14

Differential media

Answer 14

Stains colonies of specific organisms while inhibiting growth of others. Acetate agar used to differentiate between ecoli and shigella

Question 15

Obligate aerobes

Answer 15

Require oxygen to grow

Question 16

Faculatative anaerobes

Answer 16

Grows with or without Oxygen. Greater growth in the presence of oxygen

Question 17

Obligate anaerobes

Answer 17

Only grows without oxygen- anaerobic growth

Question 18

Test used to differentiate between streptococcus and staphylococcus

Answer 18

Catalase test. Staphylococcus positive

Question 19

Test used to differentiate between staphylococcus aureus and other staphylococcus

Answer 19

Coagulase test. S.aureus positive

Question 20

Kirby Bauer method

Answer 20

Disc diffusion- bacteria spread and paper discs with antibiotics placed. Zone of inhibition is measured to identify resistance

Question 21

Specimen and collection transport rules

Answer 21

Specimens collected aseptically and placed in sterile container
Some may be placed directly in culture media (blood, genital cultures)
Special handling techniques needed for some specimens (anaerobic cultures)
Prompt delivery is essential
If delayed- some can be refrigerated (urine, stool, sputum) but others must be maintained at room temp (genital, eye, Csf)

Question 22

Wet mounts must be

Answer 22

Fresh samples

Question 23

What enterobacteriase species can ferment lactose?

Answer 23

Klebsella, serratia, enterobactor, e coli