L29 Flashcards

1
Q

What drives microbiology?

A

Pathology

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2
Q

Proaryotes make how much of the Earth’s biomass?

A

20 - 50%
*9.2 - 31.7 x 10^29

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3
Q

How do we know that most organisms have not been cultured?

A

From what we have cultured using a unique fingerprint obtained from a 16S RNA mechanism, that is a protein synthesis process found in all organisms

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4
Q

What does sequencing the 16S gene help us to do?

A

Help us to find the genera of the microbe

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5
Q

How to microbes live on a grain of sand?

A

They have different metabolic processes, they oxidise the gases in the air

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6
Q

What is a population

A

When a single microbe proliferates to form many individuals

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7
Q

Community

A

Multiple populations interacting together

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8
Q

Microbial ecology

A

The study of how microbes interact with each other and their environment

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9
Q

What is enrichment culture

A

Enrichment culture - providing the temperature and
chemical conditions in the laboratory that encourage
the growth of specific groups of microbes.

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10
Q

Beggiatoa

A

chemoautotroph, fixed CO2 from the atmosphere like a plant by oxidizing an inorganic molecule

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11
Q

Mesocosm

A

an experimental system that simulates real-life conditions as closely as possible to study them in the lab

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12
Q

Two goals from 1 process

A

*Harvesting energy
*12 of the building blocks to make things

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13
Q

What do we all need?

A

An e- donor for energy, glucose –> oxidised to make CO2

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14
Q

Are bacteria versatile in their reaction pathways

A

Yes as they can have different donors and acceptors

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15
Q

What happens when H is oxidised

A

Releases protons and electrons

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16
Q

Why does O2 in our body accept the H from the NADPH

A

It gets reduced to form CO2 as it has the most redox potential

17
Q

Where does NADPH do

A

Recycled to NADP+

18
Q

What is the energy harvested from the envir turn into

A

The local currency of the body, in humans its ATP

19
Q

Molecule that serves as intermediates to transfer
energy inside of cell

A

NADH/NADPH

20
Q

Conc and ratio of NADH/NADPH

A

Conc. stays the same but ratio changes as NADH/NADPH is recycled

21
Q

Two main areas:

A

Carbon source and Energy used

22
Q

Autotrophs use _____ while heterotrophs use ____

A

CO2, Organic Carbon

23
Q

Autotrophs

A

AUTOtrophs = Primary Producers
*Fix CO2
*Self sufficient, do not require carbon

24
Q

Heterotrophs

A

HETEROtrophs = Decomposers
*Need fixed carbon,
*cannot use CO2 directly
*CO2 is oxidised

25
Q

Chemotrophs

A

use chemical energy from either e- donors:
* C compounds = organic
*Non C compounds = inorganic

26
Q

Autotrophs

A

Uses solar E at diff wavelengths to syn ATP

27
Q

Chemoautotrophs

A

Fixes an inorganic molecule with Energy from the sun
Makes own C source and then uses chem Energy to break bonds and make ATP

28
Q

Photoautotrophs

A

Fixes CO2 using E from the sun plants

29
Q

Chemoheterotrophs

A

Need Carbon molecules –> CO2 using chemical E obtained from organic/inorganic

Our Energy and Carbon source are organic carbons

30
Q

Photoheterotrophs

A

Uses light to make E but their Carbon source is not made by them

31
Q

How do microbes in the same area exploit the same resource light

A

Light = common resource
*Avoid competition by tuning their
antenna to different wavelength i.e absorb diff wavelengths