L25: Herpes Viruses

Question 1

Herpes simplex I causes?

Answer 1

cold sores on the face

Question 2

Herpes simplex II causes?

Answer 2

cold sores on the genitalia

Question 3

is herpes an enveloped virus

Answer 3

yes

Question 4

how is herpes diagnosed

Answer 4

immunofluoresnce, culture, serology (AB), PCR

Question 5

treatment of herpes

Answer 5

acyclovir

Question 6

Explain herpes virus replication

Answer 6

1. attachment by herpes envelope glycoproteins to cell surface membrane
2. entry into cytoplasm
3. release of nucleocapsid into host cell
4. transport of viral DNA to cell nucleus
5. sythesis of viral protein in cytoplasm
6. protein into host cell nucleus
7. assembly of nucleocapsid in cell nucleus and insertion of glycoproteins into nuclear membrane
8. egress of virus from infected cell with death of cell

Question 7

infection of a cell by a herpes virus can be followed by?

Answer 7

1. prompt replication of virus of cell death
2. latent infection, without viral replication
3. reactivation of latent infection with replication and cell death

Question 8

varicella zoster virus causes

Answer 8

chickenpox and shingles

Question 9

Epstein-Barr virus causes

Answer 9

glandular fever

Question 10

how is herpes simplex t1 infection acquired

Answer 10

• contaminated saliva
• replication of virus in skin and oral membranes

Question 11

latent infection of herpes simplex t1 - where, causes?

Answer 11

trigeminal nerve ganglion, cold sores

Question 12

what are some severe complications of herpes simplex

Answer 12

meningitis, encephalitis

Question 13

aquired infection of varicella zoster virus, how?

Answer 13

contaminated saliva, skin

Question 14

Replication of Varicella zoster virus in?

Answer 14

cells of skin and mucous membrane

Question 15

Varicella zoster virus latent infection?

Answer 15

dorsal root ganglia - shingles

Question 16

Epstein-Barr virus acquisition

Answer 16

infected saliva

Question 17

EBV causes infection of?

Answer 17

B cells in tonsils

Question 18

IS response to EBV

Answer 18

Cytotoxic T lymphocytes attack infected B lymphocytes – killing most of these cells.

illness with
- swollen lymph nodes and spleen
- fever
- unwellness

Question 19

Glandular fever onset

Answer 19

30 days

Question 20

symptoms of glandular fever

Answer 20

sore troat, swollen tonsils, enlarged lymph nodes, lethargy

Question 21

EBV immortalises B cells, explain

Answer 21

B cell enter and remain in growth cycle
B cells that escaped cytotoxic killing and are the reservoir for latent infection

Question 22

diagnosis of EBV

Answer 22

1. AB
   - heterophile AB that agglutinate sheep RBC
   - specific AB to EBV antigen
2. atypical number of CD8
3. DNA in sample

Question 23

vaccinations for herpes viruses

Answer 23

HSV - no
Varicella - yes
EBV - no

Question 24

varicella vaccination

Answer 24

zostavax - live attenuated vaccine
shingirx - surface protein vaccine