L18 Flashcards
memory B cells are involved in
persistent long term immunity
are polyclonal antibodies reproducible
no
why use cancerous cells to make it immortal
cancer cells continuously divide
western blot determines
quantity and band identity on a gel
elisa determiens
how much protein is present in a mixture
agglutination test determines
the identity of a pathogen
fluorescent staining is used to
localize proteins in a cell
2 ways a gel is transferred to the membrane
capillary transfer
electrophoretic transfer
blotting
getting it onto membrane
blocking
block the other sites on the membrane from antibodies that do not have anything bound to it
what binds to the blot first
primary antibody
what does the secondary antibody do in blotting
senses the primary antibody to bring in a detectable signal
what is used in isotope labelling
iodine or sulfur to label it radioactively
enzyme labelling antibodies
gives a coloured substance to identify antibodies (horseradish peroxidase or alkaline phosphatase)
fluorescent labelling of antibodies
dye attached to antibodies to see where they bind
heavy element labelling
metals that can be dented through microscopes
4 types of antibody labelling
isotope labelling
enzyme labelling antibodies
fluorescent labelling of antibodies
heavy element labelling
horseradish peroxidase does what
breaks down/activates luminol to make it glow
what gene is invloed in recycling vitamine A
RPE65
you can progressively become blind because of what
mutation in the enzyme/gene RPE65
bad thing about Elisa
doesn’t give size, just tells us of its there or not
can you have false negatives with western blotting
no - only false positives
what does Elisa stand for
enzyme linked immunosorbent assay
can multiple secondary antibodies bind a primary antibody
yes
most common Elisa test
pregnancy tests
whats the pregnancy hormone
human chorionic gonadotrophin (hCG)
SARS cov-2 antigen test tests for
the actual virus
what does Anti SARS cov-2 ing/igM test for
the presence of the antibody
