L3 Flashcards
what will high temperatures do to proteins
denature proteins making them non functional
what is conductance
how fast heat gets into something
ie, oil vs oven
what is the effectiveness of heat killing altered by (3)
temperature
time
conductance
why doesn’t milk go bad sometimes at room temp?
it has been sterilized at high temperature for a short time
what does an autoclave do to a compound
increases the boiling point by increasing pressure
what is autoclave pressure? what does that make the steam temperature?
15 PSI
121 degree
what should the things in an autoclave never be?
airtight
can you drop the pressure of an autoclave quickly
no
would cause the liquid at hot degrees would flash boil and turn into steam that could break the machine
what is a heat liable compound
breaks down when exposed to heat
why would we filter a liquid
to make it sterile because bacteria can’t fit through the pore size
how to increase the amount of detail in a microscope
improve resolution
what is angular resolution
the smallest resolvable distance between 2 objects
_________ wavelength will give better resolution
smaller
NA (numeral aperture of the lens)
want NA _________ for better resolution
large
a ________ distance, is a better resolution
smaller
whats usually the oil immersion lens
100x
what does spectroscopy mean
the study of absorption and emission of radiation
what is photometry
the measurement of intensity of radiation
what is absorbance
the amount of light that the sample blocks
what is a standard curve used for
to compare your data to a series of known samples
what is transmittance
the proportion of light that the sample allows to pass
if absorbance goes up, transmittance goes _____
down
