L10 Flashcards
whats the equation for Vo
Vo = - delta [S] / delta t
what are the 2 things the enzyme reaction rate can be limited by
availability of substrate or enzyme
time it takes for production of product
what does Km value determine
how fast it is going to get to Vmax
if an enzyme has a low Km, what do they do?
they work fast even though there is a low amount of substrate
if an enzyme has a high Km, what do they do?
they work slow until there is a lot of substrate there
what are ALDH2 and ALDH1 speed affected by
their Km values
ALDH2 is way lower Km
why would an enzyme work faster (sooner) than another?
the fast one has a lower Km value
what is competitive inhibition
something else binds the active site and blocks substrate binding
can competitive inhibition be overcome
yes by hav ing very high [S]
can competitive inhibition still get to Vmax
yes
what happens to Km during competitive inhibition
it gets higher
what happens to Vmax during competitive inhibition
Vmax doesn’t change
methanol to formaldehyde causes what
blindess and death
which Km value is lower? methanol or ethanol
ethanol
where does an uncompetitive inhibitor bind
the ES complex at a secondary location than the active site
what happens to Vmax in an uncompetitive inhibitor
goes down
what happens to Km during uncompetitive inhibitor
goes down
what does an uncompetitive inhibitor look like on a LL plot
parallel to the normal one
and up
whats an example of a competitive inhibitor
methanol
what happens during non competitive inhibition
it binds to the enzyme and changes the shape of the active site
what inhibitor is considered an allosteric regulator
non competitive inhibitor
what happened to Km during non competitive inhibition
it doesn’t change
what happens to Vmax during non competitive inhibition
lower
what inhibition is used for drug making
non competitive inhibitor
can non competitive inhibitors be selective
yes
whats a non competitive inhibitor for leukaemia
scemblix
how are enzymes quantified
by comparing the amount needed to complete a set amount of reaction in a set period of time
what are enzymes measured in
Units
