L12

Question 1

for small protein, what is Ve equal to

Answer 1

Vt

Question 2

for large protein, what is Ve equal to

Answer 2

Vo

Question 3

small protein, Kav will be closer to ___

Answer 3

1

Question 4

for large protein, Kav will be closer to ___

Answer 4

0

Question 5

what is the size of the protein proportional to in gel chromotagraphy

Answer 5

where its coming out of the column

Question 6

if Kav is >0.8 or <0.2, you will get ________ separation of proteins

Answer 6

poor

Question 7

ion exchange chromatography

Answer 7

a column of positively or negatively charged beads forms the stationary phase

Question 8

cation exchange

Answer 8

column -, targets +

Question 9

_____ charged or ____ molecules will elute quickly

Answer 9

like
neutral

Question 10

affinity chromatography

Answer 10

specific trap for protein of interest attached to stationary phase

Question 11

example of affinity chromatography

Answer 11

enzyme to substrate

Question 12

what chromatography gives us the highest purity

Answer 12

affinity chromatography

Question 13

whats the easiest chromatography to do

Answer 13

size exclusion