L2 Flashcards
what micropipettes use the same head
P200
P20
whats the volume range for micropipettes
0.5-1000ul
_______/________ =moles
mass / molar mass
_______ / ________ = molarity
moles / volume
what are the units of molarity
M or mol/L
whats the triangle for calculations look like
n
c v
whats the ratio of ml to ul
1 ml = 1000ul
what are some acidic things
lemon
apple
vinegar
tomato
banana
what are some basic things
ammonia
drain cleaner
soap
baking soda
what is happening at the pKa
50/50
half has dissociated and half is still holding a proton
where’s the best place for buffering
+/- 1 pH unit from pKa
whats the H-H equation
pH = pKa + log [base]/[acid]
what is the H-H equation used for
determine the prof a given acid/base mixture to produce a desired pH
what is the equation for PI
pI = (pK1 + pK2) / 2
where is the PI in triprotic titrations
in between -1 and +1 charges of amino acids
if the PH is less than pKa then there is __________ acid
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