Introduction to Bone Marrow Flashcards
where is red marrow at age 5-7?
yellow marrow beginning to replace red
where is the location of red marrow at birth?
all is mostly red marrow
location of red marrow in adults?
red marrow limited to “warm” sites such as the axial skeleton and proximal portions of extremeties
Where are BM samples taken from?
Prefer Posterior iliac crest (Hips and long bones) in both children and adults
the bone marrow aspirate is the ______ portion of marrow
liquid
Which sample do you perform a diff with?
the liquid aspirate
What purpose does obtaining a BM biopsy serve?
Estimate cellularity, evaluation of architecture, and allows us to perform immunohistochemical staining
Normal Myeloid:Erythroid ratio in adults
1.5:1 to 3.3:1
Normocellular formula % of cellularity
% = (100 - age) +/- 10
Comprehensive bone marrow examination includes what? (4)
- Peripheral blood smear
- bone marrow aspirates
- bone marrow core biopsy/aspirate colt selection
- ancillary studies
Ancillary studies include but not limited to… (6)
- immunohistochemistry
- special stains
- flow cytometry
- cytogenetics
- molecular
- microbiology
What does the presence of spicules on a bone marrow aspirate indicate?
indicates that the specimen was correctly collected (aka “adequate”)
Be able to identify spicules on a bone marrow
?
When performing a diff on a bone marrow aspirate, you perform counts and cellular morphology by looking where?
look around the edges of spicule, not against the spicule. Cells may be distorted!
Function of osteoblasts
- synthesize bone
