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Hematology Module 2 > Into to Flow Cytometry > Flashcards

Into to Flow Cytometry Flashcards

1
Q

CD8

A

T-cell marker

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2
Q

CD117

A

Immature marker

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3
Q

CD 71

A

Erythroid precursor marker

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4
Q

CD 19

A

B-cell marker

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5
Q

CD4

A

T-cell marker

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6
Q

Kappa light chain

A

B-cell marker

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7
Q

TdT

A

Immature marker

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8
Q

CD 41

A

Megakaryocyts marker

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9
Q

CD 13

A

Granulocytic/monocytic marker

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10
Q

CD3

A

T-cell marker

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11
Q

Glycophorin A

A

Erythroid precursor marker

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12
Q

Lambda light chain

A

B-cell marker

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13
Q

CD 34

A

Immature AND granulocytic/monocytic marker

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14
Q

CD 42

A

Megakaryocyte marker

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15
Q

CD 14

A

Granulocytic/monocytic marker

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16
Q

CD61

A

Megakaryocyte marker

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17
Q

CD 33

A

Granulocytic/monocytic marker

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18
Q

CD 20

A

B-cell marker

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19
Q

CD5

A

T-cell marker

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20
Q

CD 15

A

Granulocytic/monocytic marker

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21
Q

CD7

A

T-cell marker

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22
Q

CLL important cell morphology

A

Smudge cells

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23
Q

Mantle Cell Lymphoma important Immunophenotype

A

Cyclin D1+

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24
Q

Mantle Cell Lymphoma genetics

A

t(11;14)

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25
Q

Follicular Lymphoma important Immunophenotype

26
Q

Follicular Lymphoma genetics

27
Q

Hairy Cell Leukemia important cell morphology

A

Hairy Cells

28
Q

Hairy Cell Leukemia important immunophenotype

A

CD11c, CD103+, CD123+, bright CD20+

29
Q

Hairy Cell Leukemia genetics

A

BRAF-V600E

30
Q

Hairy Cell Leukemia important additional feature

A

Trap stain positive

31
Q

Burkitt Lymphoma important architecture

A

Diffuse with star sky pattern

32
Q

Burkitt Lymphoma important cell morphology

A

Many vacuoles

33
Q

Burkitt Lymphoma important immunophenotype

34
Q

Burkitt Lymphoma genetics

35
Q

Burkitt Lymphoma important additional features

A

Mainly in children and young adults

36
Q

Cutaneous T-Cell Lymphoma/Mycosis fungoides important architecture

A

diffuse infiltrates into skin

37
Q

Cutaneous T-Cell Lymphoma/Mycosis fungoides important cell morphology

A

sezary cells

38
Q

Cutaneous T-Cell Lymphoma/Mycosis fungoides important immunophenotype

39
Q

Large Granular Lymphocyte Leukemia important immunophenotype

A

cd4-, cd8+,cd57+, cd16+, TIA1+, Gramzyme B+

40
Q

Large Granular Lymphocyte Leukemia important genetics

A

STAT3 gene mutation

41
Q

Classical Hodgkin Lymphoma important cell morphology

A 
Reed-Sternberg (binucleated) cells 
Hodgkin Cells (mononuclear)
42
Q

Classical Hodgkin Lymphoma important immunophenotype

43
Q

Classical Hodgkin Lymphoma important additional features

A

present with localized lymph node involvement

44
Q

Plasma Cell Myeloma important architecture

A

Plasma cell infiltrates in BM >30% of cellularity

45
Q

Plasma Cell Myeloma important additional features

A

Monoclonal IgG or IgA in serum or urine; rouleaux; bone lesions

46
Q

Morphologic and functional changes are caused by what?

A

genetic and epigenetic changes

47
Q

Prominent cell type found in follicles

48
Q

Prominent cell type found in paracortex

49
Q

Prominent cell type found in medulla

A

Plasma cells

50
Q

CLL presents where and SLL presents where?

A 
CLL = bone marrow 
SLL = Lymph Nodes
51
Q

Hodgkin Lymphoma is _____ in origin

52
Q

What are the important occurrences in Hairy Cell Leukemia

A

Pamcytopenia
splenomegaly
“fried egg appearance”

53
Q

Age of onset for Burkitt Lymphoma

A

children and young adults

54
Q

Age of onset for Hodgkin Lymphoma

A

median age is 30

55
Q

lineage of plasma cells

56
Q

Flame Cells

  • morphology
  • antibody production
A
  • plasma cell

- producing IgA

57
Q

Mott Cells

  • morphology
  • antibody production
A
  • globules of immunoglobulin that stain pink, colorless or blue, inclusions called Russell Body
  • produces IgG
58
Q

Plasma cell myeloma produces what antibody(s)

A

Usually IgG, but uncommonly IgA

59
Q

Waldenstrom’s Macroglobulinemia produces what antibody(s)

60
Q

cryoglobulinemic purpura is associated with what condition?

A

Waldenstrom’s macroglobulinemia

Hematology Module 2 (14 decks)

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