Cytochemistry

Question 0

enzyme found in primary granules of neutrophils; positive in myeloid and negative in lymphoid cells

Answer 0

Myeloperoxidase Stain (MPO)

Question 1

staining constituents of cells to help determine lineage; used when cells are so immature that you cannot identify them from morphology alone

Answer 1

cytochemistry

Question 2

stains lipids; positive in primary and secondary granules of neutrophils and the granules of monocytes; negative in lymphoid cells; slightly less sensitive than MPO

Answer 2

Sudan Black Stain (SBB)

Question 3

primarily used to identify early granulocytic cells where red = positive; SPECIFIC ESTERASE

Answer 3

Naphthol-AS-D-Chloroacetate Esterase (NASDA)

Question 4

primarily used to identify monocytic cells; NONSPECIFIC ESTERASE

Answer 4

Alpha Naphthyl Butyrate/Acetate Esterase- ANBE/ANAE

Question 5

______ is positive in megakaryocytes while ______ is not

Answer 5

ANAE; ANBE

Question 6

in ANBE red is positive in ____________ cells and the _______ cells are negative.

Answer 6

monocytic; myeloid

Question 7

stains glycogen

Answer 7

Periodic Acid Schiff (PAS)

Question 8

block positivity of PAS is present in what?

Answer 8

blasts of ALL

Question 9

PAS is negative in normal RBC precursors , but positive in blasts of what?

Answer 9

erythroid type of AML

Question 10

immunoperoxidase reaction for factor VIII is positive in what?

Answer 10

megakaryoblastic leukemia

Question 11

RECAP

-MPO and SBB are positive for what cells?

Answer 11

Myeloid

Question 12

RECAP

-NASDA is specific and stains for what?

Answer 12

granulocytes

Question 13

RECAP

-ANBE is non-specific and is postive in what?

Answer 13

monocytic

Question 14

RECAP

-PAS is positive in what?

Answer 14

in ALL and Leukemic or malignant RBCs