Inhibition of Translation

Question 1

Streptomcyin

Answer 1

binds to 30S and inhibits binding of fMet-tRNA to imapair initiation (prok)

Question 2

Clindamycin and Erythromycin

Answer 2

binds to large 50S unit, blocks translocation of the ribosome (moving within the APE sites) (prok)

Question 3

Tetracyclin

Answer 3

binds small 30S subunit, blocks aminoacyl tRNA to impair elongation (prok)

Question 4

Chloramphenicol

Answer 4

inhibits peptidyl transferase to impair peptide bond formation (prok)

Question 5

Shiga Toxin and Ricin

Answer 5

binds to large 60S subunit and blocks aminoacyl tRNA from binding to ribosomal complex (euk.)

Question 6

Cycloheximide

Answer 6

inhibits peptidyl transferase to impair bond formation (euk.)

Question 7

Puromycin

Answer 7

used in lab to cause premature chain termination in both cell types

similar to the 3’ end of aminoacylated tRNA. Enters A site and adds to growing chain, causes premature stop