Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

CLINICAL BACTERIOLOGY > IDENTIFICATION OF STAPHY > Flashcards

IDENTIFICATION OF STAPHY Flashcards

1
Q

demonstrate the presence of catalase
an enzyme that catalyzes the release of oxygen from
hydrogen peroxide (H2O2)

A

Catalase Test

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

To distinguish Staphylococci spp. And Micrococci
spp. (positive)

A

Catalase Test

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

percentage of hydrogen peroxide or H2O2 in catalase test used for the routine culture

A

3%

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

percentage of hydrogen peroxide in catalase test used for detection of catalase in an aerobe

A

15%

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

During tests bacteria protect themselves from the little effect of hydrogen peroxide which is
accumulated as an end product of aerobic
carbohydrate metabolism

A

Catalase Test

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

positive result of catalase test

A

bubble formation or effervescence

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Best single pathogenicity test for staphylococcus

A

Coagulase (Slide or Tube Method)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

is a biochemical test that is used
to differentiate staphylococcus aureus from other
staphylococci species like staphylococcus
epidermidis and staphylococcus saprophyticus

A

Coagulase (Slide or Tube Method)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

a test used to detect the ability to produce the
coagulase. coagulase is an enzyme-like protein
and causes plasma to clot by converting
fibrinogen to fibrine

A

Coagulase (Slide or Tube Method)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

2 methods of coagulase test

A

Slide Method (screening test)
Tube Method (confirmatory test)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

specimen used in coagulase test

A

plasma (plasma, 0.5 mL rabbit’s plasma for tube)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

among the 2 methods of coagulase test, which one detects BOUND COAGULASE (CLUMPING FACTOR)?

A

Slide Method (screening test)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

positive results of slide method (screening test)

A

agglutination

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

among the 2 methods of coagulase test, which one detects UNBOUND COAGULASE (staphylocoagulase)?

A

Tube Method (confirmatory test)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

incubation time of tube method coagulase test

A

2hrs, 4hrs, and another 24hrs

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

positive result of tube method coagulase test

A

coagulum/clot

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

medium used in O/F reactions

A

O/F glucose medium

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

O/F glucose medium is also called as

A

Hugh Leifson medium

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

a method for biochemical
differentiation of microbes it is used to detect the
oxidation or fermentation of carbohydrates by
bacteria.

A

Oxidation-Fermentation (O/F) Reactions

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

In O/F reactions, _____ferment glucose or
fermentative, whereas _____ fail to produce
acid under anaerobic conditions.

A

Staphylococcus , micrococci

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

sugars added in O/F reaction’s medium aside from sucrose, lactose, and glucose

A

cylos, mannitol, and maltose

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

In O/F reactions, ___
utilization of the carbohydrate will result in acid
production or color yellow in the open tube only

A

Oxidative

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

In O/F reactions, ____ utilization of the carbohydrate will
result in acid production or yellow in both open
and closed rooms

A

Fermentative

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

___ organism, meaning
absence okay it will not produce acid in either of
the tube

A

asaccharolytic

25
Q

what is added in hugh leifson medium to serve as a barrier for oxygen

A

mineral oil

26
Q

In O/F reactions, if the closed tube turns yellow it means?

A

it is fermentative

27
Q

In O/F reactions, if the open tube turns yellow it means?

A

oxidative

28
Q

In O/F reactions among the genera if both tube turns yellow, is it staph or micrococci?

A

staph

29
Q

In O/F reactions, if only the open tube turns yellow. Is it staph or micococci?

A

micro

30
Q

active chemical component of modified oxidase test

A

Tetramethyl-paraphenylene diamine dihydrochloride

31
Q

is a reagent impregnated disk
recommended for use in qualitative procedures to
aid in the differentiation of staphylococcus and
micrococcus by the detection of the oxidase
enzyme

A

Microdase disk

32
Q

a positive result in modified oxidase test

A

blue to purple to black complex (micro lang ang psoitive sa modified OXIDASE test)

33
Q

medium used for mannitol fermentation

A

Mannitol Salt Agar

34
Q

positive result of mannitol fermentation

A

yellow

35
Q

negative result of mannitol fermentation

A

pink

36
Q

a yellow positive result in mannitol fermentation indicated the presence of what

A

staphylococcus aureusq

37
Q

indicator used in mannitol fermentation

A

Phenol red

38
Q

principle behind the mannitol fermentation

A

If an organism can ferment mannitol, an acidic
byproduct is formed that will cause the phenol red
in the agar to turn yellow. Most pathogenic
staphylococci such as staphylococcus aureus will
ferment mannitol and most non-pathogenic
staphylococci will not ferment mannitol

39
Q

medium used in DNAse test

A

DNAse Medium

40
Q

indicator used in DNAse test

A

methyl green

41
Q

what is added in DNAse test

A

1 N hydrochloric acid

42
Q

positive result of DNAse test

A

zone of inhibition

43
Q

a positive zone of inhibition in DNAse test identifies the presence of

A

staphylococcus aureus

44
Q

a negative result in DNAse test identifies the presence of

A

Staphylococcus epidermidis

45
Q

what percent of —- is used in gelatin medium for GELATIN LIQUEFACTION/HYDROLYSIS TEST

A

12%

46
Q

principle behind dnase test

A

If the organism that
grows in the medium produces
deoxyribonuclease, it breaks down dna into
smaller fragments. When the dna is broken down
it no longer binds to the metal grid and the green
color fades and the colony is surrounded by a
colorless zone.

47
Q

Gelatin is a protein derived from the connective
tissues of vertebrates that is ___

A

collagen

48
Q

positive result in Gelatin Liquefaction/Hydrolysis Test

A

liquefaction

49
Q

Presumptive identification of ______ is accomplished by testing for
novobiocin susceptibility

A

staphylococcus saprophyticus

50
Q

Staphylococcus saprophyticus is
__to novobiocin whereas most other coNs
are susceptible

A

resistant

51
Q

___ microgram of novobiocin disk is used in novobiocin disk

A

5

52
Q

STEPS IN COLLECTION OF BACTERIAL SAMPLE

A
  1. Use sterile cotton swab to collect bacterial sample in the skin/nasal cavity
  2. Inoculate the sample in MSA
  3. Incubate at 37C for 18-24 hours
  4. Observe for growth and color development
53
Q

STEPS IN OXIDASE TEST

A
  1. Using a sterile loop/applicator stick, rub a colony into the oxidase strip
  2. Observe for color change
    (+) Purplish to black
    (-) No color change
54
Q

STEPS IN CATALASE TEST

A
  1. Place 1 drop of 3% hydrogen peroxide reagent in a glass slide
  2. Emulsify a colony using applicator stick/inoculating loop
  3. Observe
    (+) bubble formation
    (-) no gas bubbles
55
Q

STEPS IN DNASE TEST

A
  1. Using bacteria from a plate, inoculate the DNAse medium in a straight line
  2. Incubate the DNAse medium for 18-24 hours
  3. After incubation, add a small amount of 1N HCl to the plate, flooding the colonies.
  4. Discard the excess acid and observe the media surrounding the colonies.
    (+) Clearing surrounding the colonies
56
Q

STEPS IN DNASE TEST

A

. Using bacteria from a plate, inoculate the DNAse medium in a straight line
2. Incubate the DNAse medium for 18-24 hours
3. After incubation, add a small amount of 1N HCl to the plate, flooding the colonies.
4. Discard the excess acid and observe the media surrounding the colonies.
(+) Clearing surrounding the colonies

57
Q

STEPS IN COAGULASE TEST - SLIDE

A
  1. Divide the slide into two sides using a marking pen. Label the left portion “T” (for test)
    and the right portion “C” (for control)
  2. Place a drop of plasma in the T portion of the slide
  3. Place a drop of saline in the C potion of the slide
  4. Emulsify few colonies into the T portion
  5. Emulsify few colonies into the C portion
  6. Mix well for 5 seconds and observe for clumping within 10 seconds
    (+) clumping
    (-) no clumping
    *negative control should show no clumping
58
Q

STEPS IN COAGULASE TEST - TUBE

A
  1. Add 0.5 mL of plasma into a tube
  2. Inoculate a loopful of bacteria
  3. Incubate for 2 hours and observe for clot formation
  4. If no clot is observed, incubate for another 2 hours and observe for clot formation
  5. If no clot is observed after a total of 4 hours incubation, incubate further up to 18-24
    hours
    (+) Coagulum
    (-) No coagulum

CLINICAL BACTERIOLOGY (10 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions