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CLINICAL BACTERIOLOGY > capsular staining, bacterial challenge test (disinfectants), mcfarland, ast interpretation > Flashcards

capsular staining, bacterial challenge test (disinfectants), mcfarland, ast interpretation Flashcards

1
Q

steps in capsular staining

A
  1. Place a loopful of saline on a labelled slide.
  2. Emulsion a single colony.
  3. Place a loopful of serum and spread to make a thin smear.
  4. Air dry and heat fix gently.
  5. Cover smear with Crystal Violet. Hold over flame for a few seconds
  6. Wash excess stain with 20% Aqueous CuSO4 (Copper(II) sulfate)
  7. Blot dry and view under OIO
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2
Q

reagents used in capsular staining

A

crystal violet and 20% of aqueous copper II sulfate (CuSO4)

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3
Q

BACTERIAL CHALLENGE TEST
(Effectiveness of Disinfectants)

O means

A

no growth

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4
Q

BACTERIAL CHALLENGE TEST
(Effectiveness of Disinfectants)

+ means

A

poor growth

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5
Q

BACTERIAL CHALLENGE TEST
(Effectiveness of Disinfectants)

++ means

A

good growth

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6
Q

BACTERIAL CHALLENGE TEST
(Effectiveness of Disinfectants)

+++ means

A

excellent growthq

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7
Q

C

A

Chloramphenicol

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8
Q

CN

A

Gentamicin

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9
Q

KF

A

Cephalotin

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10
Q

AM

A

Ampicillin

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11
Q

CXM

A

Cefuroxime

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12
Q

CB

A

Carbenicillin

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13
Q

TIC

A

Ticarcillin

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14
Q

TE

A

Tetracycline

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15
Q

K

A

Kanamycin

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16
Q

Ax

A

Amoxicillin

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17
Q

CIP

A

Ciprofloxacin

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18
Q

NI/F

A

Nitrofurantoin

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19
Q

ETP

A

Ertapenem

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20
Q

SAM

A

Ampicillin-Sulbactam

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21
Q

GRAM POSITIVE ANTIBIOTICS ARE THE FOLLOWING

A

GRAM POSITIVE ANTIBIOTICS ARE THE FOLLOWING

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22
Q

E

A

Erythromycin

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23
Q

AmC

A

Amoxicillin/Clavulanic
Acid

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24
Q

P

A

Penicillin G

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25
Q

S

A

Streptomycin

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26
Q

NV

A

Novobiocin

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27
Q

OX

28
Q

KF

A

Cephalotin

29
Q

ATM

30
Q

NA

A

Nalidixic Acid

31
Q

VA

A

Vancomycin

32
Q

CIP

A

Ciprofloxacin

33
Q

DA

A

Clindamycin

34
Q

FOX

35
Q

B

A

Bacitracin

36
Q

steps in bacterial challenge test (effectiveness of disinfectants)

A

Procedure:
1. Place 5ml of the following disinfectants into 3 labelled tubes
- Merthiolate
- 75% Alcohol
- Hypochlorite
2. To each tube, add 0.5 ml of bacterial suspension
3. Mix gently by tapping and start the timer
4. At the end of 30 seconds, 1 minute, and 2 minutes. Inoculate the designated area on a nutrient agar plate
5. Incubate at 35°C for 24 hours
6. Evaluate using the following:
- O = no growth
- + = poor growth
- ++ = good growth
- +++ = excellent growth

37
Q

in the bacterial challenge test, how many Celsius is the bacteria inoculated and for how many hrs?

A

35 C for 24 hrs

38
Q

0.5 McFarland standard steps

A

Procedure:
1. Prepare 1% Sulfuric acid (H2SO4) and 1.175% Barium chloride (BaCl2)
2. Using separate pipettes, add the amounts below

39
Q

how many uL are there in 1 ml

40
Q

chlorampenicol C, RIS

A

12, 17, >18

41
Q

gentamicin CN

A

13, 17, >18

42
Q

cephalotin KF

A

14, 17, >18

43
Q

ampicillin AM

A

13, 16, >17

44
Q

cefuroxime cxm

A

14, 17, > 18

45
Q

carbenicillin cb

A

19, 22, >23

46
Q

ticarcillin TICS

A

14, 19, > 20

47
Q

tetracycline TE

A

14, 18, 19

48
Q

kanamycin K

A

13, 17, >18

49
Q

amoxycillin Ax

50
Q

ciprofloxacin CIP

A

13, 17, >18

51
Q

nitrofurantoin NI/F

A

14, 16, >17

52
Q

ertapenem ETP

A

12, 15, >16

53
Q

icillin-sulbactan SAM

A

11, 14, >15

54
Q

gram negative antibiotics

A

chloramphenicol, gentamicin, cephalotin, ampicillin, cefuroxime, ticarcillin , tetracycline, kanamycin, amoxicillin, ciprofloxacin, nitrofurantoin, ertapenem, ampicillin-sulbactan

55
Q

erythromycin

A

13, 16, >17

56
Q

amoxicillin/cluvanic acid

57
Q

penicillin G

58
Q

streptomycin

A

13, 17, >18

59
Q

novobiocin

A

14,16, >17

60
Q

oxacillin

61
Q

CEPHALOTIN

A

14, 17, >18

62
Q

aztreonam

A

15, 21, >22

63
Q

nalidixic acid

A

13, 18, >19

64
Q

vancomycin

65
Q

ciprofloxaci

A

13, 17, >18

66
Q

cefoxitin

A

13, 18, >19

67
Q

bacitracin

A

8, 12, >13

CLINICAL BACTERIOLOGY (10 decks)

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