ID Flashcards
Result of TSI if ONLY glucose fermented
K/A (pink/yellow)
Initially A/A, but once glucose depleted, oxidative deamination of proteins causes pH to be alkaline on slant
BAP: NH, med gr/tan
ML: growth
glucose POS, maltose POS
N. meningitidis
Purpose of LKV (Laked blood kanamycin vancomycin)
Enhances Bacteroides
Inhibits GPC (vanco) and facul. anaerobes (kanamycin)
Beta hemolytic, spreading, yellow, matte, motile
Bacillus cereus
E. coli QC TSI result
A/A gas
Concentrations of sugars in TSI
Glucose - 0.1%
Lactose and sucrose - 1%
Taxo A = POS
CAMP = NEG
PYR POS = Group A strep
Moraxella catarrhalis rapid ID and results
NH sm gr/buff “hockey puck”
Oxidase: POS
BUTYRATE: POS
Result of NFB organism inoculated to TSI
K/NC
Will only oxidatively deaminate peptones
“Medusa, egg white, non-motile”
Bacillus anthracis
Taxo A = NEG
CAMP = NEG
Latex ACG
Result of TSI if lactose and/or sucrose also fermented
A/A (yellow/yellow)
Due to large amount of acid produced from fermentation of sugars of higher concentration
Proteus QC TSI result
K/A H2S+ gas+
CAMP = POS
Group B strep
Oxidase result of all significant gndc
POS
3 genera of H2S+ organisms
Salmonella, Proteus, Citrobacter
BEA = POS
6.5% salt = POS
Enterococci
Do PYR = POS if salt NG
Purpose of Brucella w/ neomycin
Inhibits Gram NEG facultative anaerobes`
Pseudomonas aeruginosa QC TSI result
K/NC
Pseudomonas is an NFB
Butterscotch odour
Streptococcus anginosus group
Organism JEMBEC transport is used for
N. gonorrhoeae
Catalase result for lactobacilli
NEG
Hippurate POS, tumbling motility
Listeria monocytogenes