GNDC - Neisseria and Moraxella Flashcards
Most common N. meningitidis serogroups in US?
A, B, C, W-135
2 words. This technique is used to inoculate and streak selective media for optimum recovery of GC from a charcoal transport swab. Any growth on GC selective plates must be investigated.
Z STREAK
Military, air travel increase the risk of infection by this organism
N. meningitidis
This abbreviation refers to the disease seen when Neisseria gonorrhoeae invades the bloodstream causing dermatitis-arthritis syndrome
DGI (disseminated gonococcal infection)
Added to transport media, this detoxifying agent is particulalry suitable for the recovery of Neisseria gonorrhoeae.
CHARCOAL
A male urethral direct smear of Gram-negative diplococci intracellularly is ________ for gonorrhea with 95% specificity and sensitivity in young adult males.
DIAGNOSTIC
Ox: POS
Glucose: POS
Lactose, maltose, sucrose: NEG
N. gonorrhoeae
Stands for modified (reduced vancomycin) Thayer-Martin.
MTM
Antibiotic of choice for treating Moraxella infection
SxT
Preferred empiric treatment for N. gonorrhoeae
Single, high-dose ceftriaxone (IM) + azithromycin (oral)
The species name of this gndc in which the respiratory mucous membranes become inflamed, there s a thick build up of phlegm and and associated cough
CATARRHALIS
What would cause N. meningitidis to not be groupable?
Lack of capsule
Some strains of Neisseria gonorrhoeae are sensitive to this ABX therefore may not grow on selective media such as MML or MTM.
VANCOMYCIN
If Moraxella catarrhalis has this esterase, a blue-green colour will develop on the substrate disk.
BUTYRATE
Rifampin is given as ____ of close contacts in an outbreak of N. meningitidis invasive disease. If penicillin is used the organism can re-establish after treatment is stopped.
PROPHYLAXIS
Why is isolation of N. meningitidis from URT or LRT (throat swab) reported?
Carrier status
N. meningitidis infection characterized by fever, chills and possibly a petechial rash.
MENINGOCOCCEMIA
This CNS infection is characterized by fever, chills, possibly petechial rash, neck stiffness and cognitive disfuntction. Vomiting is common especially with children.
MENINGITIS
2 drugs of choice of N. meningitidis prophylaxis
- Rifampin
- Azithromycin
If Neisseria meningitidis is isolated from a throat or sputum culture it usually does not suggest infection but rather a ____ and should be reported to indicate this possibility.
CARRIER
This media is used for Kirby-Bauer AST of N. meningitidis. The test is standardized for the addition of blood to a routine MH, and extended incubation (20-24 hrs) in CO2.
BAMH
Primary reservoir of N. meningitidis
Nasopharyngeal carriers
A neonate may present with this infection if an untreated mom with gonorrhea gives birth vaginally to her infant. For this reason, erythromycin drops are applied to neonates at birth (replacing silver nitrate).
CONJUNCTIVITIS
hemmorhagic lesions seen in DIC
PURPURA
Temperature for transporting N. gonorrhoeae
Room temperature
Common specimens for M. catarrhalis
- Sputum
- Ear drainage
- Eye swab
Moraxella cataarhalis rapid ID?
BAP: NH, buff, opaque, friable/hockey puck
CHOC: gr-tan
s/ gndc
Oxidase, Butyrate: POS
N. meningitidis on ML/CO2: G; N. gonorrhoeae on ML/CO2: G; M. catarrhalis on ML/CO2: __
NG
Moraxella catarrhalis will be negative in the four common CHO-fermentation sugars (glu, mal, lac, suc) because it is ______.
ASACCHAROLYTIC
The larger, flatter and gummier morphotypes of GC (notably on selective agar) are a result of a decrease in or lack of _____ .
PILIATION
How to report if N. gonorrhoeae cefinase test POS? NEG?
POS: report penicillin: R
NEG: don’t report
N. meningitidis emerging dominant serotype
B
Nystatin, anisomycin or amphotericin B are added to media that selects for GC. These agents are inhibitory to ____.
FUNGUS
Yet another abbreviation, this one refers to the disease seen when Neisseria gonorrhoeae ascends into the pelvis causing damage to the uterus, fallopian tubes and/ or ovaries.
PID
Antibiotic of choice to “disinfect” meningococcal carriers b/c it reaches high concentration in saliva and tear
Minocycline
This ABX is added to ML and TM to inhibit Gram-negatives.
COLISITIN
Because 10-20% of females with gonorrhoeae are _____ they are untreated. This leads to PID.
ASYMPTOMATIC
This abbreviation refers to the often fatal disease which can be seen with a variety of bacterial infections, including the meningococcus. Widespread clotting is followed by hemorrhage (depletion of clotting factors), gangrene (occlusion of blood vessels by clots), and severe hypotension and shock.
DIC
The ABX of choice for treating N. meningitidis infections - however, broad spectrum cephalosporins (cefotaxime) are administered for meningitis.
PENICILLIN
N. meningitidis agglutination test is performed with this reagent
Monoclonal antisera
N. gonorrhoeae targets this type of epithelium
COLUMNAR
This is a closed incubation system which if used with a TM will provide optimal conditions (moisture, media & CO2) for GC viability if delays in transport are inevitable.
JEMBEC
Clinical significance of Moraxella catarrhalis
- Pneumonia
- Bronchitis
- COPD
This pathogenic Neisseria species is positive for fermentation of glucose and maltose.
MENINGITIDIS
What further biochemical testing + results to perform for Moraxella ID if butyrate is NEG?
DNase: POS
Asaccharolytic
For medico-legal reasons, ______________ tests (by another method = best practice) should be performed if an isolate is identified as Neisseria gonorrhoeae.
CONFIRMATORY
Where to swab adult female for N. gonorrhoeae specimen
Endocervix
Where to get N. gonorrhoeae swab if woman has had a hysterectomy
Urethra
Name of transport tube for N. gonorrhoeae
Amies or Stuart’s transport with charcoal
Temperature for charcoal transport tube
Room temeprature
2 tests not used for N. gonorrhoeae detection anymore
CTA, CPPA
Usual treatment for N. meningitidis infection
Penicillin
