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Clinical Microbiology 1 > GPB - Coryne, Lacto, Bacillus > Flashcards

GPB - Coryne, Lacto, Bacillus Flashcards

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Biology 101 flashcards
1
Q

s/ small curved gpb, test catalase negative - a cause of pharyngitis in young adults

A

Arcanobacterium haemolyticum

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2
Q

Which Bacillus is penicillin: S?

A

B. anthracis

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3
Q

Motility of B. anthracis vs Bacillus spp.?

A

B. anthracis = non-motile

Bacillus spp. = motile

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4
Q

Erysipelothrix TSI result?

A

A/A H2S+

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5
Q

TINS: black colony with brown halo

Urease: POS

A

Corynebacterium ulcerans

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6
Q

granulomatosis infantiseptica is one of the severe manifestations of ______ - ________ disease seen with in utero infection by Listeria

A

EARLY-ONSET

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7
Q

entry via penetrating eye injury leading to endophthalmitis

A

B. cereus

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8
Q

Elek plate test purpose

A

Confirmation that an isolate is toxigenic

Used in conjunction w/ PCR for toxigenicity testing

Toxin from organism + anti-toxin (from inoculated strip) diffuse towards each other, forming lines of precipitation

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9
Q

Organism that may be AH, catalase neg, TSI is A/A H2S (+)

A

Erysipelothrix rhusiopathiae

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10
Q

Listeria macroscopic and microscopic characteristics

A
  • BH narrow-band (may be delayed)
  • sm
  • wh-gr
  • trans

Small regular gpb

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11
Q

This Bacillus species is non-motile, NH and not resistant to penicillin

A

ANTHRACIS

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12
Q

dormant resting stage of Bacillus and Clostridium

A

ENDOSPORE

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13
Q

The 3 organisms that will grow as black colonies with a brown halo on TINS

A
  • C. diptheriae
  • C. ulcerans
  • C. pseudotuberculosis
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14
Q

Clinical significance of B. cereus (3 things)

A

Food borne - emetic
Food borne - diarrheal
Ocular - endophthalmitis

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15
Q

Purpose of Loeffler’s serum slant

A

Enhance growth and morphology of C. diptheriae

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16
Q

may be added to artificial culture for the recovery of lipophilic organisms

A

TWEEN 80

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17
Q

Corynebacterium that is associated w/ UTIs

A

C. urealyticum

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18
Q

Skin test that used to be administered to determine immune status against diptheria using an intradermal injection of diptheria toxin

A

Schick test

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19
Q

TINS: black colony, no halo; urease POS

A

Corynebacterium urealyticum

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20
Q

Media made as needed to screen for C. diphtheriae

A

TINS

Due to short shelf life

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21
Q

Can have tox+ virulence factor

A

C. diptheriae

C. ulcerans

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22
Q

Bacillus undergoes _______ in adverse conditions such as heat, drying and nutritional deprivation

A

SPORULATION

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23
Q

Corynebacterium atmospheric requirements?

A

Facultative ANO2, some obligate aerobes

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24
Q

Phosphate energy storage granules (Volutin granules, Babes-Ernst granules)

A

Metachromatic granules

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25
Q

Term for the use of 4 degree incubation to select for Listeria from a heavily mixed specimen

A

COLD ENRICHMENT

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26
Q

3 Corynebacteria biotypes

A

Gravis
Intermedius
Mitis

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27
Q

Abbrev. for a nutritionally-rich fermentation detection media that supports small pH changes (weak acid production)

A

CTA

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28
Q

Often isolated in combination w/ S. aureus and S. pyogenes

A

Cutaneous diphtheria

29
Q

Some species of Corynebacterium will grow poorly or not at all on CHOC b/c they are __________

A

Lipophilic (need Tween 80 as supplement)

30
Q

Corynebacterium catalase result?

A

Positive

31
Q

one of two forms of food borne disease caused by Bacillus cereus; short incubation, often associated with infant formula and fried rice, leading to vomiting and cramping

A

EMETIC

32
Q

toxin that retains its antigenicity but not its virulence (used in immunization)

A

TOXOID

33
Q

Media containing beef serum, egg, dextrose

A

Loeffler’s serum slant

34
Q

Hemolysis of B. anthracis vs Bacillus spp.?

A

B. anthracis = NH

Bacillus spp. = BH

35
Q

Role that immunization status and low socioeconomic status plays in maintaining a reservoir of C. diptheriae

A

Humans are only reservoir

Lower socioeconomic status = overcrowding in slum area, poor access to health care = easily spread

36
Q

Biotype of C. diphtheriae causing the most severe disease

A

Gravis

37
Q

Can often be a lab contaminant

A

Bacillus

38
Q

What causes suffocation in diptheria infection?

A

Pseudomembrane (necrotic tissue, immune cells, C. dipth)

39
Q

Lactobacillus:

  • Atmospheric = ?
  • Catalase = ?
  • Normal flora sites = ?
A
  • Atmospheric = microaerobic
  • Catalase = NEG
  • Normal flora sites = vaginal flora, mouth
40
Q

2 words: one of the distinctive macroscopic morphologies often seen with B. anthracis

A

MEDUSA HEAD

41
Q

L. monocytogenes - definitive ID is confirmed with which test?

A

Hippurate test

42
Q

B. anthracis s/ ?

A

gpb large ch boxcars

May have central to subterminal spores that don’t swell cell

Most have capsule

43
Q

Describes motility in a room temp SIM for Listeria

A

Umbrella motility

44
Q

primarily an occupational hazard e.g. vets, the main pathogen in this genus, __________ , is E. rhusiopathiae, a NH ppt (24h) s/gpb (filaments) testing catalase neg

A

ERYSIPELOTHRIX

45
Q

Immediate treatment of presumptive diptheria infection?

A

Diptheria anti-toxin

46
Q

Why is Listeria a facultative intracellular parasite?

A
  • Can survive within monocytes

- Invades and multiplies in macrophages

47
Q

Resemble viridans streptococci on BAP, but s/gpb slender or spiral or Cs

Catalase NEG

A

Lactobacilli

48
Q

Presumptive ID of C. diphtheriae?

A
  • Halo on Tinsdale
  • Coryneform in colony smear
  • Urease NEG
49
Q

Corynebacterium that is very ABX resistant

A

C. jeikeium

50
Q

Least fatal disease form associated with B. anthracis, even if untreated

A

Cutaneous

51
Q

C. ulcerans on BAP (hemolysis)?

A

Weak BH, otherwise similar to C. diptheriae

52
Q

Active immunization against three pathogens including, C. diphtheriae

A

DTAP vaccine

53
Q

2 media useful in identification of Corynebacteria?

A
  • Tinsdale

- Cystine Tellurite Blood Agar (CTBA) = selective and differential for it

54
Q

on this plate test, lines of identity confirm a toxigenic strain of C. diphtheriae

A

ELEK

55
Q

Corynebacterium that is multi-resistant to most antibiotics, but vancomycin: S

A

C. jeikeium

56
Q

Term used for Corynebacterium species that are typically considered as commensals

A

DIPHTHEROIDS

57
Q

Process used to sterilize Loeffler’s serum slant?

A

Coagulation-sterilization or inspissation or fractional sterilization

58
Q

A positive in this test moves an isolate presumptively ID’d as Listeria monocytogenes by rapid ID from blood, CSF or prodcuts of conception to a definitive ID

A

Hippurate

59
Q

hippurate POS

A
  • Streptococcus agalactiae

- Listeria monocytogenes

60
Q

Media used to biotype Corynebacteria

A

Cystine-Tellurite Blood agar (CTBA)

61
Q

C. diptheriae
Cystinase = ?
Urease = ?

A 
Cystinase POS (halo on TINS)
Urease NEG
62
Q

AH, ppt, s/long slender gpb

Catalase NEG

A

Lactobacilli

63
Q

Describes the motility of Listeria at room temp in a HANGING DROP test

A

Tumbling motility

64
Q

Corynebacterium species that is never considered normal flora

A

C. diphtheriae

65
Q

Erysipelothrix disease

A

Localized cellulitis (occupational hazard due to contact w/ infected animals)

66
Q

Typical diptheroids BAP morphology

A

NH, wh, dry/waxy/crumbly (friable), very op

Looks like dry wax

67
Q

Cause of alkaline encrusted cystitis

A

C. urealyticum

68
Q

IMMEDIATE treatment for a patient presumptively diagnosed with diphtheria

A

Diphtheria anti-toxin

69
Q

s/ gpb small regular

Catalase WEAK POS

A

Listeria monocytogenes

Clinical Microbiology 1 (12 decks)

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