GPB - Coryne, Lacto, Bacillus Flashcards by E E

s/ small curved gpb, test catalase negative - a cause of pharyngitis in young adults

Arcanobacterium haemolyticum

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Which Bacillus is penicillin: S?

B. anthracis

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Motility of B. anthracis vs Bacillus spp.?

B. anthracis = non-motile

Bacillus spp. = motile

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Erysipelothrix TSI result?

A/A H2S+

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TINS: black colony with brown halo

Urease: POS

Corynebacterium ulcerans

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granulomatosis infantiseptica is one of the severe manifestations of ______ - ________ disease seen with in utero infection by Listeria

EARLY-ONSET

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entry via penetrating eye injury leading to endophthalmitis

B. cereus

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Elek plate test purpose

Confirmation that an isolate is toxigenic

Used in conjunction w/ PCR for toxigenicity testing

Toxin from organism + anti-toxin (from inoculated strip) diffuse towards each other, forming lines of precipitation

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Organism that may be AH, catalase neg, TSI is A/A H2S (+)

Erysipelothrix rhusiopathiae

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Listeria macroscopic and microscopic characteristics

BH narrow-band (may be delayed)
sm
wh-gr
trans

Small regular gpb

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This Bacillus species is non-motile, NH and not resistant to penicillin

ANTHRACIS

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dormant resting stage of Bacillus and Clostridium

ENDOSPORE

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The 3 organisms that will grow as black colonies with a brown halo on TINS

C. diptheriae
C. ulcerans
C. pseudotuberculosis

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Clinical significance of B. cereus (3 things)

Food borne - emetic
Food borne - diarrheal
Ocular - endophthalmitis

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Purpose of Loeffler’s serum slant

Enhance growth and morphology of C. diptheriae

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may be added to artificial culture for the recovery of lipophilic organisms

TWEEN 80

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Corynebacterium that is associated w/ UTIs

C. urealyticum

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Skin test that used to be administered to determine immune status against diptheria using an intradermal injection of diptheria toxin

Schick test

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TINS: black colony, no halo; urease POS

Corynebacterium urealyticum

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Media made as needed to screen for C. diphtheriae

TINS

Due to short shelf life

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Can have tox+ virulence factor

C. diptheriae

C. ulcerans

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Bacillus undergoes _______ in adverse conditions such as heat, drying and nutritional deprivation

SPORULATION

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Corynebacterium atmospheric requirements?

Facultative ANO2, some obligate aerobes

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Phosphate energy storage granules (Volutin granules, Babes-Ernst granules)

Metachromatic granules

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Term for the use of 4 degree incubation to select for Listeria from a heavily mixed specimen

COLD ENRICHMENT

3 Corynebacteria biotypes

Gravis Intermedius Mitis

Abbrev. for a nutritionally-rich fermentation detection media that supports small pH changes (weak acid production)

CTA

Often isolated in combination w/ S. aureus and S. pyogenes

Cutaneous diphtheria

Some species of Corynebacterium will grow poorly or not at all on CHOC b/c they are __________

Lipophilic (need Tween 80 as supplement)

Corynebacterium catalase result?

Positive

one of two forms of food borne disease caused by Bacillus cereus; short incubation, often associated with infant formula and fried rice, leading to vomiting and cramping

EMETIC

toxin that retains its antigenicity but not its virulence (used in immunization)

TOXOID

Media containing beef serum, egg, dextrose

Loeffler's serum slant

Hemolysis of B. anthracis vs Bacillus spp.?

B. anthracis = NH | Bacillus spp. = BH

Role that immunization status and low socioeconomic status plays in maintaining a reservoir of C. diptheriae

Humans are only reservoir Lower socioeconomic status = overcrowding in slum area, poor access to health care = easily spread

Biotype of C. diphtheriae causing the most severe disease

Gravis

Can often be a lab contaminant

Bacillus

What causes suffocation in diptheria infection?

Pseudomembrane (necrotic tissue, immune cells, C. dipth)

Lactobacillus: - Atmospheric = ? - Catalase = ? - Normal flora sites = ?

- Atmospheric = microaerobic - Catalase = NEG - Normal flora sites = vaginal flora, mouth

2 words: one of the distinctive macroscopic morphologies often seen with B. anthracis

MEDUSA HEAD

L. monocytogenes - definitive ID is confirmed with which test?

Hippurate test

B. anthracis s/ ?

gpb large ch boxcars May have central to subterminal spores that don't swell cell Most have capsule

Describes motility in a room temp SIM for Listeria

Umbrella motility

primarily an occupational hazard e.g. vets, the main pathogen in this genus, __________ , is E. rhusiopathiae, a NH ppt (24h) s/gpb (filaments) testing catalase neg

ERYSIPELOTHRIX

Immediate treatment of presumptive diptheria infection?

Diptheria anti-toxin

Why is Listeria a facultative intracellular parasite?

- Can survive within monocytes | - Invades and multiplies in macrophages

Resemble viridans streptococci on BAP, but s/gpb slender or spiral or Cs Catalase NEG

Lactobacilli

Presumptive ID of C. diphtheriae?

- Halo on Tinsdale - Coryneform in colony smear - Urease NEG

Corynebacterium that is very ABX resistant

C. jeikeium

Least fatal disease form associated with B. anthracis, even if untreated

Cutaneous

C. ulcerans on BAP (hemolysis)?

Weak BH, otherwise similar to C. diptheriae

Active immunization against three pathogens including, C. diphtheriae

DTAP vaccine

2 media useful in identification of Corynebacteria?

- Tinsdale | - Cystine Tellurite Blood Agar (CTBA) = selective and differential for it

on this plate test, lines of identity confirm a toxigenic strain of C. diphtheriae

ELEK

Corynebacterium that is multi-resistant to most antibiotics, but vancomycin: S

C. jeikeium

Term used for Corynebacterium species that are typically considered as commensals

DIPHTHEROIDS

Process used to sterilize Loeffler's serum slant?

Coagulation-sterilization or inspissation or fractional sterilization

A positive in this test moves an isolate presumptively ID'd as Listeria monocytogenes by rapid ID from blood, CSF or prodcuts of conception to a definitive ID

Hippurate

hippurate POS

- Streptococcus agalactiae | - Listeria monocytogenes

Media used to biotype Corynebacteria

Cystine-Tellurite Blood agar (CTBA)

C. diptheriae Cystinase = ? Urease = ?

``` Cystinase POS (halo on TINS) Urease NEG ```

AH, ppt, s/long slender gpb Catalase NEG

Lactobacilli

Describes the motility of Listeria at room temp in a HANGING DROP test

Tumbling motility

Corynebacterium species that is never considered normal flora

C. diphtheriae

Erysipelothrix disease

Localized cellulitis (occupational hazard due to contact w/ infected animals)

Typical diptheroids BAP morphology

NH, wh, dry/waxy/crumbly (friable), very op *Looks like dry wax*

Cause of alkaline encrusted cystitis

C. urealyticum

IMMEDIATE treatment for a patient presumptively diagnosed with diphtheria

Diphtheria anti-toxin

s/ gpb small regular Catalase WEAK POS

Listeria monocytogenes