Anaerobes

Question 1

What does an iridescent sheen on EYA growth indicate?

Answer 1

Lipase POS

Question 2

Can cause myonecrosis and severe food poisoning

Answer 2

C. perfringens

Question 3

One of the enzymes produced by microorganisms to neutralize toxic oxygen radicals (in this case with the addition of H+ to H2O2)

Answer 3

Superoxide dismutase

Question 4

B. fragilis is resistant to this substance. The test is employed both as a disc test with high potency discs K V Ct and as part of the selective media BBE.

Answer 4

Bile

Question 5

Why should possible anaerobic specimens be kept at room temperature?

Answer 5

Refrigeration can oxygenate them

Question 6

C. perfringens EYA result

Answer 6

Lecithinase POS

Lipase NEG

Question 7

This disc test is included if anaerobic gpc are isolated. With the exception of Peptostreptococcus anaerobius, the peptostreptococci are resistant.

Answer 7

SPS

Question 8

Filamentous and fungi-like

Answer 8

Actinomyces

Question 9

3 steps (treatment guidelines) for anaerobic infections

Answer 9

1. Create unfavourable (not anaerobic) environment => remove necrotic tissue, hyperbaric chamber
2. Antimicrobials to stop spread to healthy tissue
3. Neutralize toxin(s)

Question 10

C. perfringens rCAMP result

Answer 10

rCAMP POS

Question 11

This test is used to prove that an isolate is an anaerobe.

Answer 11

Aerotolerance

Question 12

4 most common antimicrobials for anaerobic infections

Answer 12

• Penicillin
• Vancomycin
• Clindamycin
• Metronidazole

Question 13

Acronym for media that is purchased reduced and anaerobically sterilized.

Answer 13

PRAS

Question 14

This filamentous gpb is associated with sulfur granules which are actually micro-colonies of the organism in vivo.

Answer 14

Actinomyces

Question 15

These anaerobes will not be recovered unless an anaerobic “glove box” (chamber) is used.

Answer 15

Strict anaerobes

Question 16

What is more important in the incubation environment for recovering anaerobes - purging O2 or maintaining a low Rh level?

Answer 16

Purging O2

Question 17

Botulism causes this type of paralysis - seen in floppy baby syndrome.

Answer 17

Flaccid

Question 18

Anaerobic gnc/gndc

Answer 18

Veillonella

Question 19

Rarest form of botulism

Answer 19

Wound botulism

Question 20

C. tetanus toxin produces this type of paralysis as seen in lock jaw.

Answer 20

Spastic

Question 21

This plate demonstrates lecithinase, lipase and proteolysis

Answer 21

EYA

Question 22

These pinpoint to small AH s/ gpb (slender, may be curly) may be facultative or obligate anaerobes. They are catalase _____.

Answer 22

Negative

Question 23

In this non-definitive test for C. perfringens, the alpha-toxin of the suspected C. perfringens isolate acts synergistically with CAMP factor from S. agalactiae to produce an arrowhead of enhanced hemolysis.

Answer 23

rCAMP

Question 24

Antimicrobial unsuitable for anaerobic infections

Answer 24

Gentamicin

Question 25

This test should be performed on anaerobic gnb to determine susceptibility to penicillins.

Answer 25

Cefinase

Question 26

1/3 of anaerobic infections are due to this group within the Bacteroides genus

Answer 26

Fragilis

Question 27

Describes the appearance of Fusobacterium nucleatum in a Gram-stain.

Answer 27

Fusiform

Question 28

A zone of <10 mm to K 1000, V 5 and Ct 10 is considered _____.

Answer 28

Resistant

Question 29

Anaerobic gnc genus

Answer 29

Veillonella

Question 30

2 redox indicators and what colour are they in anaerobic conditions?

Answer 30

- Methylene blue - Resazurin Colourless

Question 31

Blood that is frozen and thawed is described as ____ blood. It makes nutrients more readily availalbe to anaerobes and is used in LKV media

Answer 31

Laked

Question 32

This arrangement of gpb may be seen with Clostridia and Bacillus

Answer 32

Boxcars

Question 33

C. perfringens is notable for this type of gangrene.

Answer 33

Gas gangrene

Question 34

This surgical treatment removes necrotic tissue

Answer 34

Debridement

Question 35

This reducing agent is also used as the name of a broth media containing it

Answer 35

Thioglycollate