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Clinical Microbiology 1 > Anaerobes > Flashcards

Anaerobes Flashcards

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1
Q

What does an iridescent sheen on EYA growth indicate?

A

Lipase POS

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2
Q

Can cause myonecrosis and severe food poisoning

A

C. perfringens

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3
Q

One of the enzymes produced by microorganisms to neutralize toxic oxygen radicals (in this case with the addition of H+ to H2O2)

A

Superoxide dismutase

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4
Q

B. fragilis is resistant to this substance. The test is employed both as a disc test with high potency discs K V Ct and as part of the selective media BBE.

A

Bile

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5
Q

Why should possible anaerobic specimens be kept at room temperature?

A

Refrigeration can oxygenate them

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6
Q

C. perfringens EYA result

A

Lecithinase POS

Lipase NEG

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7
Q

This disc test is included if anaerobic gpc are isolated. With the exception of Peptostreptococcus anaerobius, the peptostreptococci are resistant.

A

SPS

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8
Q

Filamentous and fungi-like

A

Actinomyces

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9
Q

3 steps (treatment guidelines) for anaerobic infections

A
  1. Create unfavourable (not anaerobic) environment => remove necrotic tissue, hyperbaric chamber
  2. Antimicrobials to stop spread to healthy tissue
  3. Neutralize toxin(s)
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10
Q

C. perfringens rCAMP result

A

rCAMP POS

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11
Q

This test is used to prove that an isolate is an anaerobe.

A

Aerotolerance

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12
Q

4 most common antimicrobials for anaerobic infections

A
  • Penicillin
  • Vancomycin
  • Clindamycin
  • Metronidazole
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13
Q

Acronym for media that is purchased reduced and anaerobically sterilized.

A

PRAS

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14
Q

This filamentous gpb is associated with sulfur granules which are actually micro-colonies of the organism in vivo.

A

Actinomyces

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15
Q

These anaerobes will not be recovered unless an anaerobic “glove box” (chamber) is used.

A

Strict anaerobes

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16
Q

What is more important in the incubation environment for recovering anaerobes - purging O2 or maintaining a low Rh level?

A

Purging O2

17
Q

Botulism causes this type of paralysis - seen in floppy baby syndrome.

A

Flaccid

18
Q

Anaerobic gnc/gndc

A

Veillonella

19
Q

Rarest form of botulism

A

Wound botulism

20
Q

C. tetanus toxin produces this type of paralysis as seen in lock jaw.

A

Spastic

21
Q

This plate demonstrates lecithinase, lipase and proteolysis

A

EYA

22
Q

These pinpoint to small AH s/ gpb (slender, may be curly) may be facultative or obligate anaerobes. They are catalase _____.

A

Negative

23
Q

In this non-definitive test for C. perfringens, the alpha-toxin of the suspected C. perfringens isolate acts synergistically with CAMP factor from S. agalactiae to produce an arrowhead of enhanced hemolysis.

A

rCAMP

24
Q

Antimicrobial unsuitable for anaerobic infections

A

Gentamicin

25
Q

This test should be performed on anaerobic gnb to determine susceptibility to penicillins.

A

Cefinase

26
Q

1/3 of anaerobic infections are due to this group within the Bacteroides genus

A

Fragilis

27
Q

Describes the appearance of Fusobacterium nucleatum in a Gram-stain.

A

Fusiform

28
Q

A zone of <10 mm to K 1000, V 5 and Ct 10 is considered _____.

A

Resistant

29
Q

Anaerobic gnc genus

A

Veillonella

30
Q

2 redox indicators and what colour are they in anaerobic conditions?

A
  • Methylene blue
  • Resazurin

Colourless

31
Q

Blood that is frozen and thawed is described as ____ blood. It makes nutrients more readily availalbe to anaerobes and is used in LKV media

A

Laked

32
Q

This arrangement of gpb may be seen with Clostridia and Bacillus

A

Boxcars

33
Q

C. perfringens is notable for this type of gangrene.

A

Gas gangrene

34
Q

This surgical treatment removes necrotic tissue

A

Debridement

35
Q

This reducing agent is also used as the name of a broth media containing it

A

Thioglycollate

Clinical Microbiology 1 (12 decks)

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