Anaerobes Flashcards
What does an iridescent sheen on EYA growth indicate?
Lipase POS
Can cause myonecrosis and severe food poisoning
C. perfringens
One of the enzymes produced by microorganisms to neutralize toxic oxygen radicals (in this case with the addition of H+ to H2O2)
Superoxide dismutase
B. fragilis is resistant to this substance. The test is employed both as a disc test with high potency discs K V Ct and as part of the selective media BBE.
Bile
Why should possible anaerobic specimens be kept at room temperature?
Refrigeration can oxygenate them
C. perfringens EYA result
Lecithinase POS
Lipase NEG
This disc test is included if anaerobic gpc are isolated. With the exception of Peptostreptococcus anaerobius, the peptostreptococci are resistant.
SPS
Filamentous and fungi-like
Actinomyces
3 steps (treatment guidelines) for anaerobic infections
- Create unfavourable (not anaerobic) environment => remove necrotic tissue, hyperbaric chamber
- Antimicrobials to stop spread to healthy tissue
- Neutralize toxin(s)
C. perfringens rCAMP result
rCAMP POS
This test is used to prove that an isolate is an anaerobe.
Aerotolerance
4 most common antimicrobials for anaerobic infections
- Penicillin
- Vancomycin
- Clindamycin
- Metronidazole
Acronym for media that is purchased reduced and anaerobically sterilized.
PRAS
This filamentous gpb is associated with sulfur granules which are actually micro-colonies of the organism in vivo.
Actinomyces
These anaerobes will not be recovered unless an anaerobic “glove box” (chamber) is used.
Strict anaerobes
What is more important in the incubation environment for recovering anaerobes - purging O2 or maintaining a low Rh level?
Purging O2
Botulism causes this type of paralysis - seen in floppy baby syndrome.
Flaccid
Anaerobic gnc/gndc
Veillonella
Rarest form of botulism
Wound botulism
C. tetanus toxin produces this type of paralysis as seen in lock jaw.
Spastic
This plate demonstrates lecithinase, lipase and proteolysis
EYA
These pinpoint to small AH s/ gpb (slender, may be curly) may be facultative or obligate anaerobes. They are catalase _____.
Negative
In this non-definitive test for C. perfringens, the alpha-toxin of the suspected C. perfringens isolate acts synergistically with CAMP factor from S. agalactiae to produce an arrowhead of enhanced hemolysis.
rCAMP
Antimicrobial unsuitable for anaerobic infections
Gentamicin
This test should be performed on anaerobic gnb to determine susceptibility to penicillins.
Cefinase
1/3 of anaerobic infections are due to this group within the Bacteroides genus
Fragilis
Describes the appearance of Fusobacterium nucleatum in a Gram-stain.
Fusiform
A zone of <10 mm to K 1000, V 5 and Ct 10 is considered _____.
Resistant
Anaerobic gnc genus
Veillonella
2 redox indicators and what colour are they in anaerobic conditions?
- Methylene blue
- Resazurin
Colourless
Blood that is frozen and thawed is described as ____ blood. It makes nutrients more readily availalbe to anaerobes and is used in LKV media
Laked
This arrangement of gpb may be seen with Clostridia and Bacillus
Boxcars
C. perfringens is notable for this type of gangrene.
Gas gangrene
This surgical treatment removes necrotic tissue
Debridement
This reducing agent is also used as the name of a broth media containing it
Thioglycollate
