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Clinical Microbiology 1 > GNCB/small GNB - Haemophilus > Flashcards

GNCB/small GNB - Haemophilus Flashcards

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1
Q

an additive rich in X and V factors which is typically included in BHIB (Brain Heart Infusion Broth)

A

FILDES

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2
Q

List the HACEK speccies

A
  • H. parainfluenzae
  • Aggregatibacter aphrophilus
  • Aggregatibacter actinomycetemcomitans
  • Cardiobacterium (C. hominis predominates)
  • Eikenella corrodens
  • Kingella spp.
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3
Q

Haemophilus from CSF - drugs of choice?

A
  • Amp
  • Chloramphenicol
  • 3rd gen cephalosporin
  • Meropenem
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4
Q

Chancroid (STI)

A

Haemophilus ducreyi

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5
Q

3 genuses.

Found in oral cavity flora of humans => dental manipulation leading to endocarditis

A
  • Actinobacillus
  • Kingella
  • Cardiobacterium
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6
Q

Haemophilus that causes pink eye

A

H. aegyptius

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7
Q

Acronym for slow growing small gnb/ gncb that are occasionally isolated in SBE sub-acture bacterial endocarditis

A

HACEK

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8
Q

Makes its own V factor but not its own X factor

A

H. ducreyi

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9
Q

Epiglottitis in un-vaccinated childre 2-7 years old and males 20-30 years old

A

Haemophilus influenzae type b

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10
Q

this species of Haemophilus does not satellite but requires increased incubation time and moisture to grow and when it finally does, may be weakly BH on BAP; s/ gncb in parallel clusters (“school of fish”) and is neg in porphyrin

A

DUCREYI

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11
Q

the abbreviation for the substrate delta-aminolevulinic acid; one of the end products detected is porphyrin (Wood’s lamp UV wavelength 360 nm); theother is porphobilinogen (detected using Kovac’s)

A

ALA

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12
Q

1 organism:
BAP/SS: NH, satellites
TSA/SS: satellites

A

H. parainfluenzae

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13
Q

Meningitis in Hib vaccinated 3 year old

A

Haemophilus influnzae type a or f

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14
Q

Pasteurella multocida oxidase and indole?

A

OX: POS
Indole: Strong POS

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15
Q

Clinical significance of type b (Hib)?

A
  • Meningitis
  • Epiglottis
  • Pneumonia/septicemia
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16
Q

2 words - an increase in invasive disease seen with this Haemophilus since the introduction of Hib immunization

A

TYPE F

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17
Q

Drug of choice for infections in all sites if strain is susceptible

A

AMPICILLIN

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18
Q

a sharp decline in this invasive disease in children since the introduction of Hib immunization

A

MENINGITIS

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19
Q

Organism associated with animal bites and scratches

A

Pasteurella multocida

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20
Q

Purpose of nystatin in TH media

A

Inhibit fungi

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21
Q

Organisms that produce ________ will fluoresce brick-red using the Wood’s lamp when performing the ALA test

A

X factor

22
Q

if positive in this test, the H. influenzae strain is resistant to ampcillin via a beta-lactamase

A

CEFINASE

23
Q

Haemophilus requires this specialized media for standardized Kirby-Bauer susceptibility testing

A

Haemophilus Test Media (HTM)

24
Q

abbreviation for the true identitiy of V factor

A

NAD

25
Q

Bronchitis in adult

A

non-typeable Haemophilus influenzae

26
Q

This small gnb grows well on BAP/CO2 as decent-sized colonies but not on MAC. Infections are usually associated with a cat or dog bite or scratch (wound infection) but can get into the lungs and cause pneumonia.

A

PASTEURELLA

27
Q

term used to describe H. influenzae that have the PRP (polyribosyl-ribitol-phosphate) capsule; includes the most invasive strains

A

TYPEABLE

28
Q

this layer is pink in a positive porphyrin test

A

AQUEOUS

29
Q

Small gnb/gncb causing sub-acute bacterial endocarditis

A

HACEK group

30
Q

true identity of one of the compounds found by lyzing red blood cells - known as X factor

A

HEMATIN

31
Q

What’s HTM composed of?

A

MH media with added:

  • yeast extract
  • hematin
  • NAD
32
Q

How are BLNAR strains detected?

A

AST by an MIC method

33
Q

This species of Haemophilus will be BH satelliting on BAP and POS in Pophyrin test

A

PARAHEMOLYTICUS

34
Q

2 organisms:

POS in porphyrin (ALA positive)

A
  • H. parainfluenzae

- H. parahaemolyticus

35
Q

abbreviation for the media used for standardized AST of Haemophilus; incubate in CO2 for 16-18 hrs

A

HTM

36
Q

BAP/SS/CO2: +4 NH sm-ppt, satellites s/gncb, small gnb

CHOC/CO2: +4 sm beige-tan dry s/ gncb, small gnb

ALA: POS

A

Haemophilus parainfluenzae

37
Q

This acronym is used to describe H. influenzae strains that lack the beta-lactamase but have chromosomally-mediated altered PBP; this mechanism of resistance would not be picked up by a cefinase test

A

BLNAR

38
Q

Haemophilus mechanism of ampicillin resistance?

A

By beta-lactamase via plasmid

39
Q

This large fusiform gnb is a strict capnophile. The canimorsus species exhibits colonies that spread gently away from the streak line because of gliding motility and is associated with life-threatening infections following dog-bites.

A

CAPNOCYTOPHAGA

40
Q

Difference between H. influenzae and a BLNAR strain

A

BLNAR has a chromosomally-mediated altered binding site for ampicillin

41
Q

This species of Aggregatibacter (once part of the haemophili) doesn’t require X and is variable for V.

A

APHROPHILUS

42
Q

if this specimen grew H. influenzae you would perform AST for ampicillin, chloramphenicol, a 3rd gen ceph and meropenem

A

CSF

43
Q

Paesteurella multocida growth on BAP, CHOC, MAC?

A

BAP: G
CHOC: G
MAC: NG

44
Q

Can cause pneumonia in an elderly patient

A

H. influenzae NON-TYPEABLE

45
Q

this upper layer is pink if the reaction in the ALA test is due to indole

A

SOLVENT

46
Q

What does BLNAR stand for?

A

Beta-lactamase negative ampicillin-resistant

47
Q

The conjugate vaccine introduced in 1987 is effective against this strain (abbreviation) FYI conjugated to protein to improve immunogenicity

A

HIB

48
Q

Clinical significance of nontypeaable virulence adhesins?

A
  • Otitis media
  • Sinusitis
  • Pneumonia/bronchitis
49
Q

NH small gncb/small gnb that requires V factor but not X factor may be reported from sputum as a potential cause of..?

A

Pneumonia (H. parainfluenzae)

50
Q

2 organisms:

Require both X and V

A
  • H. influenzae

- H. haemolyticus

51
Q

Why is it routine to do cefinase ASAP for Haemophilus?

A

Because drug of choice = ampicillin

Clinical Microbiology 1 (12 decks)

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