Hydrolysis Of Starch Flashcards
What does amylase do?
Promotes the hydrolysis of starch to sugar
(Helps convert starch to sugar)
Do all bacteria produce Amylase?
Y/N
NO
What type of test is used in order to determine the presence of starch in the media after inoculation and incubation.
Simple starch test
What is added to the medium for testing presence of starch ?
What color will it turn if starch is present?
What color will it turn if starch has been hydrolyzed to sugar?
-Iodine is added (grams iodine)
-dark purple if starch is present
-not change color if starch hydrolyzes to sugar.
Is starch a polysaccharide?
Y/N?
Yes
What exoenzyme causes starch to turn to sugar?
Amylase
What organisms are used in hydrolysis of starch ?
-bacillus spizizenii
-Escherichia coli
At what tempurature do you incubate ?
37 degrees Celsius
What is the recommended incubation time?
48 hours
If incubation last more than ______ days what can occur?
4 days
False positive
How do you know if the organism is positive for amylase production ?
What does this mean ?
-no color change will occur in iodine
-amylase converted starch into sugar
How do you know organism is negative for amylase ?
-iodine will turn dark purple around streak
Is sugar a poly saccharide or disaccharide?
Disaccharide
What is the reagent used in hydrolysis of starch test ?
Iodine
Color for positive appearance for amylase?
Iodine color around streak
(No color change)
