HEMATOLOGY Flashcards
Scientific study of blood and its component
Help physicians diagnose abnormalities in the blood
Hematology
standard procedure of blood collection using lancets & needles of varying gauges
Phlebotomy
Type of puncture: capillaries (lancets)
Skin puncture
Type of puncture: veins (needles)
Venipuncture
Type of puncture: arteries (needles)
Arterial puncture
Not a common practice in the field of hematology. Usually reserved for patients that have difficult veins to extract.
Often performed by physicians.
Arterial puncture
The force of ________ will separate the different components of the blood.
centrifugation
Plasma
Extracellular Fluid
Buffy Coat & Erythrocytes
Formed Elements
Buffy Coat
Leukocytes & Platelets
55% of whole blood
plasma
least dense component
plasma
white blood cells
leukocytes
platelets
thrombocytes
<1% of whole blood
Buffy coat
red blood cells
erythrocytes
45% of whole blood
RBC/ Erythrocytes
most dense component
RBC/ Erythrocytes
WBC concentration
Neutrophil - 65%
Lymphocyte - 23%
Monocyte - 5%
Eosinophil - 4%
Basophil - 1%
Liquid portion of blood
Plasma
H2O in Plasma
91.5%
Under Plasma
Albumin - 54%
Globulin - 38%
Fibrinogen - 7%
Pale/ Clear yellow liquid- intravascular/ extracellular
plasma
Water, sugar, fat, protein and salt solution
55% of a normal human’s blood volume
plasma
transports RBC, WBC and platelets through the blood vessels
Remove waste products of metabolism
Plasma
Major component of the formed element
RBC/ Erythrocyte
anucleated cells produced from the bone marrow
RBC
gas transporting protein molecule; gives blood red color
Hemoglobin
When hemoglobin mixes with oxygen (color is bright red)
Oxyhemoglobin
form of hemoglobin without oxygen. Carries carbon dioxide (blood becomes darker)
Deoxyhemoglobin
Bright red
Arterial Blood
Dark red
Venous Blood
low number of RBCs
anemia
high number of RBCs
polycythemia
Nucleated cells that lacks haemoglobin
WBC (Leukocyte)
Acts as defense against infection
WBC (Leukocyte)
Most numerous
Multi-lobed nucleus
Pale lilac granules
NEUTROPHIL
WBC: Function: immune defense
protect the body from infection by killing and ingesting bacteria, fungi and foreign bodies
NEUTROPHIL
Spherical nucleus
“robin’s egg blue” cytoplasm - staining characteristic
LYMPHOCYTE
3 types of Lymphocyte
T-cell
B-cell
NK cells
Lymphocyte that acts as cellular immune response; recognizes foreign substances and processes them for removal
T-cell
kills cancer cells
NK cells (Natural Killer)
Usually with a bilobed nucleus - staining characteristic
Granules stain bright reddish-orange
EOSINOPHIL
defense against parasites
Activate allergic response - immune response
EOSINOPHIL
With purple-blue granules - obscure the nucleus
BASOPHIL
WBC functions as an Inflammatory response
Involved in allergic response
BASOPHIL
They will present antigens by digesting the pathogen and place a remnant of the antigen on their surface and present it to lymphocytes for recognition
Macrophage
Large leukocytes
Kidney-shaped or horse shoe-shaped nucleus
MONOCYTE
marks out cells that are antigens (foreign bodies) that should be destroyed by lymphocytes
Dendritic cell
act as antigen-presenting cells
Free wandering - roam around the body to hunt down foreign agents that might have entered our body.
Primary purpose is to engulf, destroy, and phagocytize foreign agents.
Macrophage
Cell fragments
Platelets
form clots during injury to prevent blood from leaking out
Platelets
form clots during injury to prevent blood from leaking out
Platelets
When you injured yourself, the _______ will aggregate to form a clot, thereby preventing the blood to leak out from the body.
Platelets
rbc indices
Mean Corpuscular Volume (MCV)
Mean Corpuscular Hemoglobin (MCH)
Mean Corpuscular Hemoglobin Concentration (MCHC)
iron-containing oxygen transport metalloprotein in the red blood cells
hemoglobin
reagent in Cyanmethemoglobin Method
Drabkin’s Reagent
instrument in Cyanmethemoglobin Method
Spectrophotometer (540 nm)
measures change in color
Spectrophotometer (540 nm)
oxidation of ferrous iron to ferric by potassium ferricyanide = methemoglobin
methemoglobin converted to cyanmethemoglobin with cyanide ions
Cyanmethemoglobin Method
Utilizes cyanmethemoglobin method with
modified Drabkin’s reagent
Automated Hemoglobinometry
HemoCue method
Modified azidemethemoglobin reaction
Point-of-care (POC) Hemoglobin Assay
Reagent in Point-of-care (POC) Hemoglobin Assay
sodium nitrite and sodium azide
Also known as packed cell volume (PCV) or erythrocyte volume fraction (EVF)
Hematocrit Determination
For evaluation or treatment of anemia and determine presence of nutritional deficiencies
Hematocrit Determination
Hemoglobin Determination
a) Cyanmethemoglobin Method
b) Automated Hemoglobinometry
c) Point-of-care (POC) Hemoglobin Assay
Hematocrit Determination
a) Spun microhematocrit
b) Automated
Manual procedure
Blood collection method: skin puncture
Spin a blood-filled capillary tube using a microhematocrit centrifuge
Spun for about 3-5 mins
Spun microhematocrit
Computed from the mean cell volume and the red cell count
Automated
A machine that measures the red area
microhematocrit tube reader.
Automated Blood Cell Count
Electrical Impedance
Optical Detection
- also known as Coulter Principle
- sizing and counting of particles is based on changes in electrical resistance creating voltage pulses
Electrical Impedance
- Hydrodynamic focusing method
- Uses laser light in cell counting and sizing
Optical detection
young RBCs without nucleus but still bears cytoplasmic RNA
Reticulocytes:
Determines how the bone marrow produce and release new RBCs to compensate lost/damaged RBCs
Reticulocyte count
Rate at which RBCs fall in a column
Erythrocyte sedimentation rate
Non-specific test for inflammation
Erythrocyte sedimentation rate
Erythrocyte sedimentation rate: Reference Method
Westergren Method
Erythrocyte sedimentation rate: Anticoagulant
sodium citrate
required for the blood not to clot, and allow the formed elements to settle at the bottom
sodium citrate
Erythrocyte sedimentation rate:
one hour
PLASMA COAGULATION ASSAYS
Prothrombin Time (PT) and Activated Partial Thromboplastin Time (APTT)
Coagulation testing
Detect abnormalities in hemostasis
PLASMA COAGULATION ASSAYS
Prothrombin Time (PT) and Activated Partial Thromboplastin Time (APTT)
Anticoagulant: PLASMA COAGULATION ASSAYS
sodium citrate
initiate blood clotting.
Prothrombin
Lymphocyte: antibody production. So that foreign agents can easily be killed, trapped, or phagocytized.
B-cell