GRAM (-) AEROBIC COCCI Flashcards
All NEISSERIA are OXIDASE (+) except
N. elongata
Coffee/kidney-bean shaped with flattened sides;
NM, diplococcic, capnophilic, non-hemolytic
Catalase (+) except N. elongata
Growth inhibitor: fatty acids
GRAM NEGATIVE AEROBIC COCCI
Confirmatory test for gram (-) aerobic cocci
used to ferment various bacteria
CHO Utilization Test
Medium: CTA Medium
Indicator: Phenol Red
CHO Utilization Test
Transport Media
Amie’s with charcoal
Transgrow
JEMBEC
fatal bacterial meningitis; epidemic meningococcal meningitis (meningococcemia)
Neisseria meningitidis
Incubation period of Neisseria meningitidis
4 days
Waterhouse Friderichsen syndrome
Hemorrhage
Bean-shaped diplococcic, NS,NM
Extracellular/Intracellular
Virulence factor: capsule / liposaccharide
Ferments: Glucose and Maltose
Preferred Specimen: Nasal Pharyngeal swab
Carrier: nasophraynx
Enzyme substrate test: Gammaglutamylaminopeptidase
Neufeld-Quellung (+)
Neisseria meningitidis
never considered as normal flora
Always In Transport Medium
Man is always known host
Incubation : 2 - 7 days
Neisseria gonorrhoeae
Intracellular diplococci, kidney/coffee bean
NM, NS, NE
Sensitive to drying and temperature extremes
Neisseria gonorrhoeae
Ferments Glucose
Principal virulence factor: Pili / Fimbriae
DNASE (-)
Primary Isolation Sites:
Male: Urethra
Female: Endocervix
Neisseria gonorrhoeae
Fitz Hugh Curtis Syndrome
Superoxol test - uses 30% hydrogen peroxide
Enzyme Substrate test: PPNG
Presence of prolyl-hydroxyprolylaminopeptidase
Superoxol: presumptive fast
Can be mistaken for N. meningitidis
Ferments glu, lac, mal,
Enzyme substrate test: beta-galactosidase
N. Lactamica
Can be mistaken for N. gonorrhoeae
Colistin Susceptibility
N. cinerea
