GRAM (-) AEROBIC COCCI Flashcards
All NEISSERIA are OXIDASE (+) except
N. elongata
Coffee/kidney-bean shaped with flattened sides;
NM, diplococcic, capnophilic, non-hemolytic
Catalase (+) except N. elongata
Growth inhibitor: fatty acids
GRAM NEGATIVE AEROBIC COCCI
Confirmatory test for gram (-) aerobic cocci
used to ferment various bacteria
CHO Utilization Test
Medium: CTA Medium
Indicator: Phenol Red
CHO Utilization Test
Transport Media
Amie’s with charcoal
Transgrow
JEMBEC
fatal bacterial meningitis; epidemic meningococcal meningitis (meningococcemia)
Neisseria meningitidis
Incubation period of Neisseria meningitidis
4 days
Waterhouse Friderichsen syndrome
Hemorrhage
Bean-shaped diplococcic, NS,NM
Extracellular/Intracellular
Virulence factor: capsule / liposaccharide
Ferments: Glucose and Maltose
Preferred Specimen: Nasal Pharyngeal swab
Carrier: nasophraynx
Enzyme substrate test: Gammaglutamylaminopeptidase
Neufeld-Quellung (+)
Neisseria meningitidis
never considered as normal flora
Always In Transport Medium
Man is always known host
Incubation : 2 - 7 days
Neisseria gonorrhoeae
Intracellular diplococci, kidney/coffee bean
NM, NS, NE
Sensitive to drying and temperature extremes
Neisseria gonorrhoeae
Ferments Glucose
Principal virulence factor: Pili / Fimbriae
DNASE (-)
Primary Isolation Sites:
Male: Urethra
Female: Endocervix
Neisseria gonorrhoeae
Fitz Hugh Curtis Syndrome
Superoxol test - uses 30% hydrogen peroxide
Enzyme Substrate test: PPNG
Presence of prolyl-hydroxyprolylaminopeptidase
Superoxol: presumptive fast
Can be mistaken for N. meningitidis
Ferments glu, lac, mal,
Enzyme substrate test: beta-galactosidase
N. Lactamica
Can be mistaken for N. gonorrhoeae
Colistin Susceptibility
N. cinerea
Yellow pigment
Asaccharolytic
Ability to grow on BAP or CAP at 22C
N. flavescens
Colony on CAP similar to N. gonorrhoeae
Colistin Susceptibility
N. cinerea
Ferments glu and mal
Growth on NA at 35 C & production of
polysaccharide
N. polysaccharea
mistaken as N. meningitidis
N. polysaccharea
wrinkled and bread crump colony
Ferments glucose, maltose, sucrose
N. sicca
Normal flora in dogs
Primary isolation site: wounds from dog bites
Catalase (+)
N. weaveri
Mostly non-pathogenic
Found in GIT of animals
Anaerobic
Veillonella
they are not able ferment sugars
Normal flora of oropharynx and nasopharynx
Moraxella catarrhalis
