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BACTE - BACTERIAL STRUCTURE > CULTURE MEDIA > Flashcards

CULTURE MEDIA Flashcards

1
Q

Commonly used base media

A

Agar

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2
Q

any SUBSTANCE that has the nutritional rqmts. for bacterial growth

A

culture media

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3
Q

only 1 organism is grown

A

pure culture

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4
Q

1 or 2 organisms are grown

A

mixed culture

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5
Q

made of pure culture in -20 / 70degC

A

Stock culture

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6
Q

Inverted position inside the ref

A

Prepared media

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7
Q

Stored in room temperature

A

Dry/Dehydrated media

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8
Q

PRAS

A

Pre-reduced Anaerobically Sterilized Media

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9
Q

Anaerobic Culture

A

PRAS
Gas Pak Jar
Candle Jar

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10
Q

Consistency of Liquid media

A

broth

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11
Q

Consistency of Semi-solid media

A

0.5-1% agar

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12
Q

Consistency of solid media

A

3-2% agar

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13
Q

How the medium is dispensed

A
  • Plated
  • Tubed
    - Slant
    - Butt
    - Butt/Slant
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14
Q

enhances the propagation of bacteria

A

Enrichment Media

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15
Q

Enrichment Media for Salmonella and Proteus

A

Tetrathionate broth

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16
Q

Enrichment Media for Vibrio spp.

A

Alkaline Peptone Water

17
Q

Medium that contains nutritive supplement ex. is blood

A

Enriched Media

18
Q

Warming of Thioglycollate medium

A

warm in water bath to remove oxygen used
in anaerobes; pink at the top

19
Q

distinguishes organisms growing together by their differences in cultural characteristics

A

Differential medium

20
Q

Example of Differential medium

A

Mackonkey, SSA, EMB

21
Q

promotes the growth of desirable organisms but at the same time inhibiting the growth of the other microorganism

A

Selective Media

22
Q

Example of Selective Media

A

BSA, SSA, MAC, XLD, MSA, CTA, LJ,HEKTOEN

23
Q

distinguishes organisms growing together by their differences in cultural characteristics

A

Selective & Differential

24
Q

Example of Selective & Differential

A

EMB, MacConkey, TCBS

25
Q

Specific Media for Leptospira

A

Fletcher media

26
Q

Specific Media for Listeria monocytogenes

A

Mcbride

27
Q

Specific Media for Mycobacterium tuberculosis (MTB)

A

Lowenstein-Jensen

28
Q

Specific Media for Neisseria

A

Thayer Martin

29
Q

Transport Media

A

CATS

Cary-Blair
Amie’s
Transgrow
Stuart’s

30
Q

Antibiotic Susceptibility Medium

A

MHA (Mueller Hinton Agar)

31
Q

Inhibts gram (+) organisms

A

Gentian/crystal violet
Sodium deoxycholate & other bile salts

32
Q

Inhibits gram (-) organisms

A

Potassium tellurite
Sodium azide

33
Q

Prevents swarming of Proteus

A

Alcohol
Chloral hydrate

BACTE - BACTERIAL STRUCTURE (15 decks)

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