Glycolysis

Question 1

What regulates glucose entering the cell?

Answer 1

Insulin

Question 2

First step in glycolysis

Answer 2

Glucose to glucose-6-Pi (irreversable)

Question 3

Where is hexokinase found?

Answer 3

All tissue types

Question 4

Where is glucokinase found?

Answer 4

Liver and pancreatic beta cells

Question 5

What is inhibited by G-6-P?

Answer 5

Hexokinase

Question 6

What is the function of glucokinase?

Answer 6

to remove glucose from the blood

Question 7

What is the committed step of glycolysis?

Answer 7

F-6-P to F-1,6-BP. Uses one ATP. Is irreversible. Phosophofructokinase 1 (PFK1)

Question 8

What inhibits enolase?

Answer 8

Fluoride, used to clinically determine BS

Question 9

Aerobic glycolysis

Answer 9

Pyruvate converted to acetyl-CoA

Question 10

What is the third irreversable reaction of glycolysis?

Answer 10

PEP to pyruvate (pyruvate kinase). ATP formed

Question 11

Anaerobic glycolysis

Answer 11

Lactate dehydrogenase forms Lactate

Question 12

What is the primary purpose of anaerobic glycolysis?

Answer 12

Regeneration of NAD+

Question 13

Why must NAD+ be regenerated for glycolysis?

Answer 13

NADH cannot cross the mitochondrial membrane

Question 14

ATP production in glycolysis?

Answer 14

net gain of 2 ATP per a glucose molecule

Question 15

What activates PFK-1?

Answer 15

F-2,6-BP, AMP

Question 16

What inhibits PFK-1?

Answer 16

Citrates, ATP

Question 17

What activates Pyruvate kinase?

Answer 17

F-2,6-BP, AMP

Question 18

What inhibits pyruvate kinase?

Answer 18

Acetyl-CoA, ATP

Question 19

What is 2-3BPG’s affect on erythrocytes?

Answer 19

Decreased affinity for O2, so O2 is released

Question 20

What type of carb metabloism is not affected by insulin?

Answer 20

Fructose metabolism

Question 21

Glactosemia

Answer 21

lack of G-1-P uridyltransferase