Gluconeogenesis

Question 1

RLE of gluconeogenesis

Answer 1

Fructose-1,6-Bisphosphatase

Question 2

enzyme: Pyruvate to oxaloacetate

Answer 2

pyruvate carboxylase

with biotin

Question 3

enzyme: oxaloacetate to PEP

Answer 3

PEP carboxykinase

Question 4

enzyme: F1,6BP to F6P

Answer 4

F1,6BPase

Question 5

G6P to glucose

Answer 5

glucose-6-phosphatase

Question 6

Where does gluconeogenesis take place?

Answer 6

Hepatocytes
Kidney
Intestinal epithelium

*Cells that are designed to share glucose when there is low glucose

Question 7

Hepatocytes do two main things in low glucose levels

Answer 7

Share glycogen stores

Create pyruvate –> glucose

Question 8

Source of pyruvate for gluconeogenesis

Answer 8

Odd chain FAs generating propionyl CoA
TCA molecules
Amino acids

Question 9

Gibbs free energy formula

Answer 9

ΔG = ΔG - TΔS

G = Gibbs free energy
H = energy/enthalpy (heat change in constant pressure rxns)
S = entropy 

ΔG is amount of energy released by exergonic reaction

Question 10

Irriversible enzymes in gluconeogenesis

Answer 10

pyruvate carboxylase
PEP carboxykinase
fructose-1,6-bisphosphatase
glucose-6-phosphatase