Generating Diversity I Flashcards
1
Q
What is a gene?
A
a region of DNA containing the code for a protein or one of its parts
2
Q
What does “degeneracy in the genetic code” mean?
A
synonyms in the codons mean one amino acid has multiple codons
3
Q
What is transcription?
A
info in DNA made into an RNA copy, then RNA polymerase uses one DNA strand as the template to make a polypeptide mRNA
4
Q
What is translation?
A
uses info from RNA to create polypeptides from amino acids using ribosomes (creates chain of amino acids)
5
Q
What are the 5 steps for DNA sequencing?
A
- DNA purification
- DNA fragmentation
- Amplification of fragments
- Sequencing each fragment
- Assembling fragment sequences into genome sequences
6
Q
What is the Sanger method?
A
it is based on deoxyribonucleotides
7
Q
What is the Illumina/Solexa method?
A
allows up to 1 billion DNA fragments to be sequenced simultaneously
