G6PDH : PPP's rxn 1

Question 1

G6PDH structure

Answer 1

hG6PDH dimer and tetramer at eq. , and with both active.

In active site, NADP+ binds and G6P’s a-H adds to its si face (NADP+’s si face). This is an a-H because it is on the opposite site of the arm .

Question 2

Liver isozyme

Answer 2

∆G = -17.6 kj/mol so it is the regulatory step

Question 3

mech

Answer 3

something removes H+ from His side chain N which causes e- shift to pull a H+ off of C1 OH (tautomeric shift), produces 6-phosphogluconolactone.