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Immunology And Serology > Febrile Antigens > Flashcards

Febrile Antigens Flashcards

1
Q

stained bacterial antigen suspensions used to identify and measure antibodies following infection

A

febrile antigens

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2
Q

the bacteria cause the body to produce the antibody in response to what

A

pathogens created

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3
Q

what is the positive reaction when serum sample is mixed with a febrile antigen?

A

agglutination

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4
Q

serologic tests performed when dealing with febrile antigen

A

widal test, weil-felix test

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5
Q

specifically for the detection of antibodies from typhoid

A

widal test

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6
Q

what is the causative agent of typhoid?

A

salmonella enterica subsp. enterica serovar typhi

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7
Q

test specifically for the detection of antibodies for Rickettsial infection

A

Weil-Felix test

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8
Q

what is used in Weil-Felix test as bacterial suspension

A

Proteus

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9
Q

for the widal test, what are the 4 antigen suspensions used?

A

s. typhi O
s. typhi H
s. typhi BH
s. typhi AH

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10
Q

what is the negative control for the widal test?

A

normal saline solution

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11
Q

in weil-felix test, what are the 3 antigen suspension used?

A

Proteus OX2
Proteus OX19
Proteus OXK

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12
Q

Proteus associated with P. vulgaris

A

Proteus OX2

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13
Q

Proteus associated with P. mirabilis

A

Proteus OX19

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14
Q

Proteus associated with Kingsbury strain

A

Proteus OXK

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15
Q

what is utilized as an antigen in Widal test?

A

killed salmonella typhi

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16
Q

where does the salmonella typhi grow?

A

intestines, blood

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17
Q

mode of transmission of typhoid

A

eating or drinking food or water contaminated with feces of an infected person

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18
Q

what are the dominant antigens that we deal with in widal test?

A

antigen O, antigen H

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19
Q

somatic antigen
heat stable

A

antigen O

20
Q

flagellar antigen
heat labile

A

antigen H

21
Q

what is the sample of choice in widal test?

A

serum

22
Q

what vacutainers are used for the specimen of widal test?

A

non-additive vacutainer (red or yellow top)

23
Q

what drug is taken by the patient while waiting for the result of the Widal test and cultures of blood and stool

A

chloramphenicol

24
Q

Vi antigen (capsule/ virulence)
thermolabile, unstable, delicate

A

K antigen

25
Q

among all the antigens of S. typhi, what is used for reference laboratories?

A

K antigen

26
Q

is the positive result of rapid slide screening method an indication of S. typhi infection?

A

no

27
Q

enumerate the degree of agglutination for widal rapid slide titration method

A

4+ 100% (solid clumps)
3+ 75%
2+ 50%
1+ 25%
Trace less than 25%
Negative no agglutination

28
Q

(WIDAL’S TEST rapid slide titration) serum volume and approximation tube dilution

A

0.08ml 1:20
0.04ml 1:40
0.02ml 1:80
0.01ml 1:160
0.005ml 1:320

29
Q

all positive results obtained through the slide test should be confirmed using the following technique

A

Widal’s tube agglutination test

30
Q

incubation time and temperature in water baths for salmonella “O” antigens and proteus

A

50C for 4 hours

31
Q

incubation time and temperature in water baths for salmonella “H” antigens and proteus

A

50C for 2 hours

32
Q

antibody excess

A

prozone

33
Q

antigen excess

A

postzone which causes negative test result

34
Q

characterisitc of positive result in widal’s tube agglutination test

A

obvious floccular agglutination throughout the tube

35
Q

considered significant titer in the widal’s test

A

1:80

36
Q

considered significant titer for weil-felix test

A

1:160

37
Q

what causes a stronger agglutination reaction which is not easily dispersed between the types of agglutination test in widal’s

A

somatic agglutination

38
Q

fluffy and floccular aggregates that are easily dispersed

A

flagellar agglutination

39
Q

significant titer for flagellar agglutination

A

greater than 80 = indicates infection

40
Q

indicates acute/recent typhoid fever

A

INC titer on OD Ag

41
Q

indicates acute/recent paratyphoid infection

A

INC titer on OA, OB, OC

42
Q

indicates previous/ past infection with typhoid fever
convalescent/vaccination against typhoid fever

A

INC titer on HD Ag

43
Q

indicates past infection on S. paratyphi

A

INC titer on HA, HB, HC

44
Q

indicates mixed infection

A

INC titer on O and H Ag

45
Q

what causes typhus fever?

A

R. prowazekii

46
Q

what is the mechanism used in weil-felix test

A

cross reaction mechanism

47
Q

why does weil-felix test use proteus?

A

because of the cross-reaction mechanism; rickettsia and proteus possess the same antigenic determinants

Immunology And Serology (11 decks)

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